1,153 research outputs found
Dynamical nonequilibrium molecular dynamics reveals the structural basis for allostery and signal propagation in biomolecular systems
A dynamical approach to nonequilibrium molecular dynamics (D-NEMD), proposed in the 1970s by Ciccotti et al., is undergoing a renaissance and is having increasing impact in the study of biological macromolecules. This D-NEMD approach, combining MD simulations in stationary (in particular, equilibrium) and nonequilibrium conditions, allows for the determination of the time-dependent structural response of a system using the Kubo–Onsager relation. Besides providing a detailed picture of the system’s dynamic structural response to an external perturbation, this approach also has the advantage that the statistical significance of the response can be assessed. The D-NEMD approach has been used recently to identify a general mechanism of inter-domain signal propagation in nicotinic acetylcholine receptors, and allosteric effects in \upbeta -lactamase enzymes, for example. It complements equilibrium MD and is a very promising approach to identifying and analysing allosteric effects. Here, we review the D-NEMD approach and its application to biomolecular systems, including transporters, receptors, and enzymes
Usefulness of Liver and Spleen Acoustic Radiation Force Impulse (ARFI) for the evaluation of cirrhotic patients
Objective: To evaluate the correlation between ARFI and Child-Pugh classification. Secondary
Aims: 1) To compare ARFI values (hepatic, splenic and spleno-hepatic index) from cirrhotic to
normal population; 2) To correlate biochemical parameters of liver function and ARFI. Materials
and Methods: 58 cirrhotic patients (referenced to US for surveillance or to clarify any hepatic decompensation)
were included in this prospective study, as well as 38 healthy subjects who underwent
ultrasonography for other reasons than hepatic evaluation. All had ARFI liver and spleen
evaluation on ACUSON S2000 ARFI equipment. The best cut-off liver and spleen values for the diagnosis
of cirrhosis in comparison to the normal subjects were determined using SPSS® v20. Results:
Mean liver ARFI values in controls and cirrhotic patients were respectively 1.18 ± 0.22 m/s
and 2.93 ± 0.50 m/s. The ROC curve demonstrated an AUC 0.998 and the best cut-off was 1.89.
Mean spleen ARFI values in controls and cirrhotic patients were respectively 2.60 ± 0.42 m/s and
3.03 ± 0.71. The ROC curve demonstrated an AUC 0.766 and the best cut-off was 2.73 m/s. The
splenohepatic index showed a worse AUC than ARFI liver. A weak correlation was found between
the ARFI liver and Child-Pugh. We found no statistically significant differences for spleen ARFI
values and Child-Pugh. We found a statistically significant correlation between liver ARFI and bilirubin,
ALKP, GGT, AST and AST/ALT ratio; and with spleen ARFI and ALKP and AST/ALT ratio.
Conclusion: We showed that there is a tendency of higher levels of liver ARFI values for higher
Child-Pugh classification suggesting a definite trend for higher values with more severe disease
Identification of the Initial Steps in Signal Transduction in the α4β2 Nicotinic Receptor:Insights from Equilibrium and Nonequilibrium Simulations
Formulações de hidrogel de dextrino para a regeneração de defeitos ósseos e não-uniões de fraturas
National Patent (INPI)A presente invenção consiste em formulações, à base de hidrogel de dextrino, que servem de matriz de suporte e veículo de substituto ósseos granulares para regeneração óssea. especificamente, esta invenção consiste em hidrogéis injetáveis e biorreabsorvíveis, de preparação in situ, os quais atuam como um suporte tridimensional (scaffold) para a colonização celular. a referida formulação contem granulados bioativos osteogénicos (cerâmicos bioativos) à base de fosfatos de cálcio, hidroxiapatite ou mistura de ambos, que atuam como agentes osteocondutores, permitindo assim o crescimento e regeneração tecidual óssea, funcionando a conjugação hidrogel-cerâmico como um substituto ósseo injetável.info:eu-repo/semantics/publishedVersio
Synchrotron X-ray studies on polyamide composites prepared by reactive injection molding
Semicrystalline polyamide 6 (PA6) and composites on its basis are among the most frequently used polymer materials for highly demanding applications. The performance of these composites depends on the crystalline structure of the PA6 matrix in which two crystalline forms most frequently coexist: α- and γ-polymorphs. This work reports on the crystalline structure of a variety of composite materials produced by in-mold reactive polymerization of caprolactam in specially designed semi-automatic equipment for reactive processing of nylons (NYRIM), carried out in the presence of particulate mineral reinforcements (natural or-ganically treated aluminum silicates and synthetic titanosilicates), PA6 oriented monofilaments and textile structures of glass fibers. The morphology and the crystalline structure of all composites were studied by syn-chrotron X-ray diffraction. Transcrystalline PA6 layer was observed in all fibrous PA6 laminates whose struc-ture fine crystalline structure was accessed.Fundação para a Ciência e Tecnologia; German Synchrotorn Radiation Source - DESY, Hambur
Potential association of HCF164, a chloroplast nuclear-encoded thioredoxin-like protein, with Coffea SH9 resistance factor against Hemileia vastatrix
29th Conference of Association for the Science and Information on Coffee, 11 Sept. - 14 Sept. 2023 Hanoi, Vietnaminfo:eu-repo/semantics/publishedVersio
An integrated in vitro approach unveils the biocompetence and glutathiolomic profile of a human hepatocyte-like cell 3d model
Funding: This work was supported by FCT (Portugal) through the research grant PTDC/MED-TOX/29183/2017. Acknowledgments: The authors thank ECBio S.A. for providing the hnMSCs and F.A. Beland (NCTR, Jefferson, AR, USA) for the kind donation of nevirapine. FCT (UID/DTP/04138/2019, UID/QUI/00100/2019, RECI/QEQ-MED/0330/2012, SFRH/BD/144130/2019 to J.S.R., SFRH/BD/110945/2015 to P.F.P. and CEECIND/02001/2017 to A.M.M.A) are also acknowledged.The need for competent in vitro liver models for toxicological assessment persists. The differentiation of stem cells into hepatocyte-like cells (HLC) has been adopted due to its human origin and availability. Our aim was to study the usefulness of an in vitro 3D model of mesenchymal stem cell-derived HLCs. 3D spheroids (3D-HLC) or monolayer (2D-HLC) cultures of HLCs were treated with the hepatotoxic drug nevirapine (NVP) for 3 and 10 days followed by analyses of Phase I and II metabolites, biotransformation enzymes and drug transporters involved in NVP disposition. To ascertain the toxic effects of NVP and its major metabolites, the changes in the glutathione net flux were also investigated. Phase I enzymes were induced in both systems yielding all known correspondent NVP metabolites. However, 3D-HLCs showed higher biocompetence in producing Phase II NVP metabolites and upregulating Phase II enzymes and MRP7. Accordingly, NVP-exposure led to decreased glutathione availability and alterations in the intracellular dynamics disfavoring free reduced glutathione and glutathionylated protein pools. Overall, these results demonstrate the adequacy of the 3D-HLC model for studying the bioactivation/metabolism of NVP representing a further step to unveil toxicity mechanisms associated with glutathione net flux changes.publishersversionpublishe
Resistance to the “Last Resort” Antibiotic Colistin:A Single-Zinc Mechanism for Phosphointermediate Formation in MCR Enzymes
A hyperpromiscuous antitoxin protein domain for the neutralization of diverse toxin domains
Toxin–antitoxin (TA) gene pairs are ubiquitous in microbial chromosomal genomes and plasmids as well as temperate bacteriophages. They act as regulatory switches, with the toxin limiting the growth of bacteria and archaea by compromising diverse essential cellular targets and the antitoxin counteracting the toxic effect. To uncover previously uncharted TA diversity across microbes and bacteriophages, we analyzed the conservation of genomic neighborhoods using our computational tool FlaGs (for flanking genes), which allows high-throughput detection of TA-like operons. Focusing on the widespread but poorly experimentally characterized antitoxin domain DUF4065, our in silico analyses indicated that DUF4065-containing proteins serve as broadly distributed antitoxin components in putative TA-like operons with dozens of different toxic domains with multiple different folds. Given the versatility of DUF4065, we have named the domain Panacea (and proteins containing the domain, PanA) after the Greek goddess of universal remedy. We have experimentally validated nine PanA-neutralized TA pairs. While the majority of validated PanA-neutralized toxins act as translation inhibitors or membrane disruptors, a putative nucleotide cyclase toxin from a Burkholderia prophage compromises transcription and translation as well as inducing RelA-dependent accumulation of the nucleotide alarmone (p)ppGpp. We find that Panacea-containing antitoxins form a complex with their diverse cognate toxins, characteristic of the direct neutralization mechanisms employed by Type II TA systems. Finally, through directed evolution, we have selected PanA variants that can neutralize noncognate TA toxins, thus experimentally demonstrating the evolutionary plasticity of this hyperpromiscuous antitoxin domain
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