The phase plane of moving discrete breathers

We study anharmonic localization in a periodic five atom chain with quadratic-quartic spring potential. We use discrete symmetries to eliminate the degeneracies of the harmonic chain and easily find periodic orbits. We apply linear stability analysis to measure the frequency of phonon-like disturbances in the presence of breathers and to analyze the instabilities of breathers. We visualize the phase plane of breather motion directly and develop a technique for exciting pinned and moving breathers. We observe long-lived breathers that move chaotically and a global transition to chaos that prevents forming moving breathers at high energies.Comment: 8 pages text, 4 figures, submitted to Physical Review Letters. See http://www.msc.cornell.edu/~houle/localization

Composite prepreg application device

A heated shoe and cooled pressure roller assembly for composite prepreg application is provided. The shoe assembly includes a heated forward contact surface having a curved pressure surface. The following cooled roller provides a continuous pressure to the thermoplastic while reducing the temperature to approximately 5.degree. C. below glass transition temperature. Electric heating coils inside the forward portion of the shoe heat a thermoplastic workpiece to approximately 100.degree. C. above the glass transition. Immediately following the heated contact surface, a cooled roller cools the work. The end sharpened shape of the heated shoe trailing edge tends to prevent slag buildup and maintain a uniform, relaxed stress fabrication

Pulse Propagation in Chains with Nonlinear Interactions

Pulse propagation in nonlinear arrays continues to be of interest because it provides a possible mechanism for energy transfer with little dispersion. Here we show that common measures of pulse dispersion might be misleading; in strongly anharmonic systems they tend to reflect a succession of extremely narrow pulses traveling at decreasing velocities rather than the actual width of a single pulse. We present analytic estimates for the fraction of the initial energy that travels in the leading pulses. We also provide analytic predictions for the leading pulse velocity in a Fermi-Pasta-Ulam beta-chain

Identification of the water quality factors which prevent fingernail clams from recolonizing the Illinois River—phase III

TECHNICAL COMPLETION REPORT Project No. B-124-ILL Agreement No. 14-34-0001-0217The purpose of this research was to determine why fingernail clams have been unable to recolonize a 100-mile reach of the Illinois River where they were abundant prior to a die-off in the 1950's. Fingernail clams are major links in food chains leading from detritus and algae to higher level consumers valued by man, such as fish and water fowl. Three suspected toxicants and sediments from the reach where the die-off occurred were tested on intact fingernail clams (Musculium transversum) and gill preparations isolated from the clams. Concentrations of fluoride, lead and cadmium which caused a 50% reduction in the rate of beating of cilia on isolated clam gills, after 10 minutes of exposure (10-minute EC50), were 0.75, 0.02 and 0.06 mg/l , respectively. Mixtures of cadmium and fluoride were slightly more toxic to clam gills than predicted from results of bioassays with single toxicants. A fluoride concentration of 2.82 mg/l killed intact fingernail clams after eight weeks of exposure, while mortality in lesser concentrations and one higher concentration did not differ significantly from controls maintained in well water to which no fluoride had been added. Hence, the sub-lethal response exhibited by the gills is at least four times more sensitive than the lethal response. Maximum fluoride concentrations reported by the U.S. Geological Survey at two stations in the Illinois River ranged from .6 to .8 mg/l between 1979 and 1981, considerably below the concentrations which affected growth and survival of intact clams during 8-week exposures in our laboratory, but slightly above the level which affected isolated clam gills. A lead bioassay using intact clams was completed, but the results were ambiguous because concentration ranges in separate test chambers overlapped. In addition, insoluble lead precipitates accumulated in the test chambers, and the relative toxicity to clams of the soluble versus the insoluble lead was not determined. Until additional bioassays are completed, it is impossible to determine whether the maximum total lead concentrations of 0.40 mg/l which occurred in the Illinois River between 1979 and 1981 could have contributed to the failure of fingernail clams to recolonize the river. Fingernail clams exposed to sediments from lakes along the Illinois River suffered greater mortality after six weeks of exposure than clams exposed to sediment from the Mississippi River, although the differences were not statistically significant. The same sediments tested on clam gills produced statistically significant changes in ciliary beating rate and particle transport rates on the gills. Sediments from the upstream lakes cause a greater depression in the particle transport rate and ciliary beating rates than sediments from downstream lakes. In addition, sediments from the lake furthest upstream caused a drastic change from the normal metachronal beating pattern to an atypical synchronous pattern. The results with the gill assay suggest that sediments in the Illinois River contain unidentified toxic factors and that sediments in the upper river, closer to the metropolitan areas of Joliet and Chicago, are more toxic than sediments further downstream. These results should be confirmed by additional tests with intact clams, including field tests with caged organisms. Parallel chemical analyses and bioassays of extracts from the sediments should be performed to identify the toxic components.U.S. Department of the InteriorU.S. Geological SurveyOpe

Non-rectangular towpreg architectures

A shaped towpreg ribbon having a cross-sectional geometry which promotes intimate lateral contact between adjacent composite tows was prepared. The cross-sectional geometry is non-rectangular and promotes intimate lateral contact between adjacent towpreg ribbons during normal processing

QUALITY CONTROL FROM A SUBSET OF HUMAN SURGICAL TISSUE SPECIMENS FROM THE IU SIMON CANCER CENTER TISSUE PROCUREMENT AND DISTRIBUTION CORE COLLECTED IN 2009-2010: AN H&E AND RIN VALUE ASSESSMENT

poster abstractQuality control (QC) of human tissue specimens for research is critical for the development of new bio-markers and their ability to determine clinical trial outcomes. In this study, we evaluated sixty-nine samples for both RNA and histology quality control measures from the IU Simon Cancer Center Tissue Bank. The IU Simon Cancer Center Tissue Bank is a centralized tissue procurement resource established to collect high quality tissue for basic clinical and translational research, collecting approximately 550 clinical cases per year using an informed consent and HIPAA signed document. All tissues are collected and processed in liquid nitrogen within 30 minutes of removal. The tissue samples are sliced and diced into 100 to 150 mg sample size. Each sample is placed into individual 2ml cryovials. Two representative samples are placed in 10% neutral buffered formalin. Two investigators QC the slides by microscopy to evaluate the following: percent of tumor, percent of necrosis, percent of fibrosis/inflammation, and percent of normal adjacent tissue. RNA was extracted using the Purescript RNA isolation kit (Gentra). Fifty-four of sixty-nine cases passed both histology and RNA (RIN value) QC. Of the fifteen cases that did not pass our QC criteria, thirteen cases did not pass the histology QC due to lack of tumor content (below 50%) in the sample, while the remaining two cases failed the RNA QC. Seventy-eight percent of samples passed our QC measures. The results were consistent with the existing literature on tissue quality control in human surgical tissue specimens

Perfluorooctanoic acid exposure triggers oxidative stress in the mouse pancreas

• PFOA triggers focal ductal hyperplasia following 7 day exposure. • PFOA exposure increases 8-iso-PGF2α levels in the pancreas. • Antioxidant gene expression is upregulated in the pancreas following PFOA exposure. , Perfluorooctanoic acid (PFOA) is used in the manufacture of many industrial and commercial products. PFOA does not readily decompose in the environment, and is biologically persistent. Human epidemiologic and animal studies suggest that PFOA exposure elicits adverse effects on the pancreas. While multiple animal studies have examined PFOA-mediated toxicity in the liver, little is known about the potential adverse effects of PFOA on the pancreas. To address this, we treated C57Bl/6 mice with vehicle, or PFOA at doses of 0.5, 2.5 or 5.0 mg/kg BW/day for 7 days. Significant accumulation of PFOA was found in the serum, liver and pancreas of PFOA-treated animals. Histopathologic examination of the pancreas revealed focal ductal hyperplasia in mice treated with 2.5 and 5.0 mg/kg BW/day PFOA, while inflammation was observed only in the high dose group. Elevated serum levels of amylase and lipase were observed in the 2.5 mg/kg BW/day PFOA treatment group. In addition, PFOA exposure resulted in a dose-dependent increase in the level of the lipid peroxidation product 8-iso-PGF2α and induction of the antioxidant response genes Sod1, Sod2, Gpx2 and Nqo1. Our findings provide additional evidence that the pancreas is a target organ for PFOA-mediated toxicity and suggest that oxidative stress may be a mechanism through which PFOA induces histopathological changes in the pancreas

On modulational instability and energy localization in anharmonic lattices at finite energy density

The localization of vibrational energy, induced by the modulational instability of the Brillouin-zone-boundary mode in a chain of classical anharmonic oscillators with finite initial energy density, is studied within a continuum theory. We describe the initial localization stage as a gas of envelope solitons and explain their merging, eventually leading to a single localized object containing a macroscopic fraction of the total energy of the lattice. The initial-energy-density dependences of all characteristic time scales of the soliton formation and merging are described analytically. Spatial power spectra are computed and used for the quantitative explanation of the numerical results.Comment: 12 pages, 7 figure

Non-rectangular towpreg architectures

A shaped towpreg ribbon having a cross-sectional geometry which promotes intimate lateral contact between adjacent composite tows was prepared. The cross-sectional geometry is non-rectangular and promotes intimate lateral contact between adjacent towpreg ribbons during normal processing

ELEVATED LEVELS OF PLATELETS AND MDM2 EXPRESSION ARE CONTRIB-UTING FACTORS TO FACILITATING THE METASTASIS OF OSTEOSARCOMA

poster abstractOsteosarcoma (OS) is the most common form of primary bone cancer and the 6th leading cause of cancer in pediatric patients. A chart review of OS patients treated at this institution suggests that a high platelet count at di-agnosis is significantly (p=0.023) and inversely associated with the first year of survival. As the effects of platelet interaction with OS have been exten-sively researched and suggest that platelets may facilitate tumor metastasis, and the most important prognostic factor for OS patient survival is metasta-sis to the lungs, we hypothesized that platelets increase metastasis to the lungs and reduce survival. Therefore, we sought to determine whether in-creasing platelet numbers in a well characterized OS mouse model would de-crease survival and/or increase metastasis to the lungs. We found that thrombopoietin (TPO) treated mice, had increased platelet numbers, died earlier than placebo treated controls, and that lungs from TPO treated mice contained a small number of large tumor cells (most metastatic lesions were 2-4 cells), whereas lungs from placebo treated controls showed no signs of metastases. Next, an OS tissue microarray (TMA) was built from OS patients seen at our institution over the past 10 years. Mdm2, p53, TPO, and c-mpl expression were evaluated by immunohistochemical (IHC) staining followed by quantitation using the Aperio Imaging system and analysis software. C-mpl (TPO receptor) expression was higher in the metastatic than the primary tumors, suggesting that platelets may contribute to the metastasis of OS. Elevated levels of Mdm2 correlated with metastasis and lower levels of p53, as detected by IHC. In conclusion, both the mouse model and the human OS data were similar, suggesting that both platelets and Mdm2 promote metas-tases in OS