292 research outputs found
Discovery of an unusual bright eclipsing binary with the longest known period: TYC 2505-672-1 / MASTER OT J095310.04+335352.8
We report on the MASTER Global Robotic Net discovery of an eclipsing binary,
MASTER OT J095310.04+335352.8, previously known as unremarkable star TYC
2505-672-1, which displays extreme orbital parameters. The orbital period
P=69.1 yr is more than 2.5 times longer than that of epsilon-Aurigae, which is
the previous record holder. The light curve is characterized by an extremely
deep total eclipse with a depth of more than 4.5 mag, which is symmetrically
shaped and has a total duration of 3.5 yrs. The eclipse is essentially gray.
The spectra acquired with the Russian 6 m BTA telescope both at minimum and
maximum light mainly correspond to an M0-1III--type red giant, but the spectra
taken at the bottom of eclipse show small traces of a sufficiently hot source.
The observed properties of this system can be better explained as the red giant
eclipsed by a large cloud (the disk) of small particles surrounding the
invisible secondary companion.Comment: 8 figures, 9 pages, Astronomy and astrophysics in prin
Photometric observations of the supernova 2009nr
We present the results of our UBVRI CCD photometry for the second brightest
supernova of 2009, SN 2009nr, discovered during a sky survey with the
telescopes of the MASTER robotic network. Its light and color curves and
bolometric light curves have been constructed. The light-curve parameters and
the maximum luminosity have been determined. SN 2009nr is shown to be similar
in light-curve shape and maximum luminosity to SN 1991T, which is the prototype
of the class of supernovae Ia with an enhanced luminosity. SN 2009nr exploded
far from the center of the spiral galaxy UGC 8255 and most likely belongs to
its old halo population. We hypothesize that this explosion is a consequence of
the merger of white dwarfs
Optical polarization observations with the MASTER robotic net
We present results of optical polarization observations performed with the
MASTER robotic net for three types of objects: gamma-ray bursts, supernovae,
and blazars. For the Swift gamma-ray bursts GRB100906A, GRB110422A, GRB121011A,
polarization observations were obtained during very early stages of optical
emission. For GRB100906A it was the first prompt optical polarization
observation in the world. Photometry in polarizers is presented for Type Ia
Supernova 2012bh during 20 days, starting on March 27, 2012. We find that the
linear polarization of SN 2012bh at the early stage of the envelope expansion
was less than 3%. Polarization measurements for the blazars OC 457, 3C 454.3,
QSO B1215+303, 87GB 165943.2+395846 at single nights are presented. We infer
the degree of the linear polarization and polarization angle. The blazars OC
457 and 3C 454.3 were observed during their periods of activity. The results
show that MASTER is able to measure substantially polarized light; at the same
time it is not suitable for determining weak polarization (less than 5%) of dim
objects (fainter than 16). Polarimetric observations of the optical
emission from gamma-ray bursts and supernovae are necessary to investigate the
nature of these transient objects.Comment: 31 pages, 12 figures, 4 tables; Exposure times in Table 2 have been
correcte
Comparative characterization of Leporipoxvirus members’ reproduction in continuous cell culture
Examination of the virus-cell interactions is of both scientific and practical importance. Our study was aimed at comparative characterization of rabbit myxoma virus and Shope fibroma virus biological properties that manifested during the virus reproduction in RK-13/2-03 clonal continuous rabbit kidney cell culture. It was demonstrated that the viruses varied in infection development and cytopathic effect duration in RK-13/2-03 cell culture. Apparent lesions in cell monolayers infected by myxoma virus and fibroma virus at similar multiplicity of infection and cultivation temperature were observed on day 2 and day 3 of cultivation, respec- tively, as well as maximum cell lesions with evident degeneration were observed on day 3 and day 6 of cultivation, respectively. Myxoma virus was accumulated at titre of 6.25–6.50 lg TCID50/0,2 cm3, and Shope fibroma virusа was accumulated at titre of 5.50−5.75 lg TCID50/0.2 cm3. Shope fibroma virus demonstrated such infectivity during three passages and myxoma virus demonstrated such infectivity during twenty passages. Prepared cultures were identified as myxoma virus and Shope fibroma virus with molecular genetic analysis. Tests of the viruses for their antigenic relatedness showed that antibodies against myxoma virus were able to neutralize Shope fibroma virus also. NT titres of antibodies against both viruses were similar (1:8). RK-13/2-03 cell culture was found to be highly permissive to Shope fibroma virus that had been isolated from the diseased rabbit and not been an attenuated variant
Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi
Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply. The search for the most effective AMF strains for symbiosis and the creation of microbial preparations on that basis is an important task for modern biology. Owing to the difficulties of cultivation without a host plant and their high genetic polymorphism, identifying AMF is very difficult. The high number of cryptic species often also makes morphological identification unreliable. Recent years have seen growth in the number of AMF biodiversity studies performed by modern NGS-based methods, Illumina MiSeq in particular. Currently, there are still many questions that remain for the identification of AМF. The most important are whether conservative or variable sequences should be used to select a marker for barcoding and whether universal primers or those specific to AMF should be used. In our work, we have successfully used universal primers ITS3 and ITS4 for the sequencing in Illumina MiSeq of the 5.8S rDNA – ITS2 region of the 35S rRNA genes, which contain both a conservative and variable regions. The molecular genetic approach for AMF identification was quite effective and allowed us to reliably identify eight of nine isolates to the species level: five isolates of Rhizophagus irregularis, and one isolate of R. invermaius, Paraglomus laccatum, and Claroideoglomus etunicatum, respectively. For all five R. irregularis isolates high variability in the ITS region and the absence of ecotopic-related molecular characters in the ITS2 region were demonstrated. The NCBI data is still insufficient for accurate AMF identification of Acaulospora sp. isolates from the genus to the species level
SWEET transporters of Medicago lupulina in the arbuscular-mycorrhizal system in the presence of medium level of available phosphorus
Arbuscular mycorrhiza (AM) fungi receive photosynthetic products and sugars from plants in exchange for contributing to the uptake of minerals, especially phosphorus, from the soil. The identification of genes controlling AM symbiotic efficiency may have practical application in the creation of highly productive plant-microbe systems. The aim of our work was to evaluate the expression levels of SWEET sugar transporter genes, the only family in which sugar transporters specific to AM symbiosis can be detected. We have selected a unique “host plant–AM fungus” model system with high response to mycorrhization under medium phosphorus level. This includes a plant line which is highly responsive to inoculation by AM fungi, an ecologically obligate mycotrophic line MlS-1 from black medick (Medicago lupulina) and the AM fungus Rhizophagus irregularis strain RCAM00320, which has a high efficiency in a number of plant species. Using the selected model system, differences in the expression levels of 11 genes encoding SWEET transporters in the roots of the host plant were evaluated during the development of or in the absence of symbiosis of M. lupulina with R. irregularis at various stages of the host plant development in the presence of medium level of phosphorus available for plant nutrition in the substrate. At most stages of host plant development, mycorrhizal plants had higher expression levels of MlSWEET1b, MlSWEET3c, MlSWEET12 and MlSWEET13 compared to AM-less controls. Also, increased expression relative to control during mycorrhization was observed for MlSWEET11 at 2nd and 3rd leaf development stages, for MlSWEET15c at stemming (stooling) stage, for MlSWEET1a at 2nd leaf development, stemming and lateral branching stages. The MlSWEET1b gene can be confidently considered a good marker with specific expression for effective development of AM symbiosis between M. lupulina and R. irregularis in the presence of medium level of phosphorus available to plants in the substrate
Massive stars exploding in a He-rich circumstellar medium. IV. Transitional Type Ibn Supernovae
We present ultraviolet, optical and near-infrared data of the Type Ibn
supernovae (SNe) 2010al and 2011hw. SN 2010al reaches an absolute magnitude at
peak of M(R) = -18.86 +- 0.21. Its early light curve shows similarities with
normal SNe Ib, with a rise to maximum slower than most SNe Ibn. The spectra are
dominated by a blue continuum at early stages, with narrow P-Cygni He I lines
indicating the presence of a slow-moving, He-rich circumstellar medium. At
later epochs the spectra well match those of the prototypical SN Ibn 2006jc,
although the broader lines suggest that a significant amount of He was still
present in the stellar envelope at the time of the explosion. SN 2011hw is
somewhat different. It was discovered after the first maximum, but the light
curve shows a double-peak. The absolute magnitude at discovery is similar to
that of the second peak (M(R) = -18.59 +- 0.25), and slightly fainter than the
average of SNe Ibn. Though the spectra of SN 2011hw are similar to those of SN
2006jc, coronal lines and narrow Balmer lines are cleary detected. This
indicates substantial interaction of the SN ejecta with He-rich, but not
H-free, circumstellar material. The spectra of SN 2011hw suggest that it is a
transitional SN Ibn/IIn event similar to SN 2005la. While for SN 2010al the
spectro-photometric evolution favours a H-deprived Wolf-Rayet progenitor (of
WN-type), we agree with the conclusion of Smith et al. (2012) that the
precursor of SN 2011hw was likely in transition from a luminous blue variable
to an early Wolf-Rayet (Ofpe/WN9) stage.Comment: 23 pages, 11 figures, 6 tables. Accepted by MNRA
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