Pharmacological studies of the veterinary medicinal product “Dibutalastin Ointment”

The study of pharmacological studies of the veterinary medicinal product for external use in the form of ointment – “Dibutalastin Ointment” is a mandatory stage of preclinical research of the drug, which is a new development of PP “Biopharm” and LLC “DEVIE”. In a scientific experiment on laboratory animals, it is possible to assess the drug's safety for different terms of use to determine the zone of toxic action and doses that do not cause harmful effects on health. Therefore, the work aimed to carry out pharmacological studies of the drug with methyl salicylate and dimethyl sulfoxide in the form of an ointment for treating European fallow deer, deer, dogs, and cats with mastitis, arthritis, myositis, and injuries of various origins. The toxicological characteristics of the studied ointment “Dibutalastin” based on methyl salicylate and dimethyl sulfoxide were studied in an "acute" study on warm-blooded animals. The average lethal dose (LD50) for intragastric administration could not be established, as the volume exceeded the permissible level. It was established that according to the “average lethal dose when entering the stomach” indicator, the veterinary medicinal product belongs to relatively non-toxic substances (toxicity class IV). The results of pharmacological studies indicate the absence of resorptive-toxic effects, sensitizing properties, and irritating effects on the mucous membranes of the eyes and skin. Pathomorphological studies of animal organs when studying the acute toxicity of the drug showed that the developed ointment with methyl salicylate and dimethyl sulfoxide does not cause pathological changes in internal organs. From the point of view of veterinary toxicology, this drug is safe. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic effect of “Dibutalastin Ointment”, which is mandatory material of the “Safety and residue studies” section of the dossier for this medicinal product

Imidazoline receptor agonists and left ventricular hypertrophy

The role of sympathic autonomous system in arterial hypertension (AH) pathogenesis is described. Predictive value of left ventricular hypertrophy (LVH) in cardiovascular event prognosis is emphasized. The results of clinical trials assessing imidazoline receptor agonists’ influence on LVH during AH management are discussed

Vascular and platelet hemostasis and microcirculation in arterial hypertension patients

The article is focused on the role of endothelium and vasoactive endothelial factors in arterial hypertension (AH) development. Special attention is given to platelet functional state, blood rheology, and microcirculatory disorders’ role in AH pathogenesis

Environmental factors’ influence on blood pressure variability and circadian rhythm in arterial hypertension patients

Aim. Using twin models, to demonstrate the leading role of environmental factors for blood pressure (BP) variability (Var) and circadian rhythm (CR) parameters. Material and methods. In total, 74 twin pairs with normal BP (mean age 42,4±2,3 years) and 64 twin pairs with arterial hypertension (AH) (mean age 52,76±3,2 years) were examined. Simultaneous pair-wise 24-hour BP monitoring (BPM) was performed with АВРМ-02 device (“MEDITECH”, Hungary). Var of mean systolic BP (VMSBP), mean diastolic BP (DBP), mean hemodynamic BP (VMBP) and mean pulse BP (VPBP) was investigated, as well as Var of mean heart rate (HR) and circadian indices (CI) for SBP, DPB and MBP. Results. In participants with normal BP, environmental factors played the leading role in forming BP Var parameters. In AH patients, hereditary factors input in daytime VPBP was about 21%. Nighttime VSBP and VMBP were genotype-influenced by 22% and 36%, respectively. Random environmental factors were more influential for CIin AH patients; in AH-free individuals, environmental and hereditary factors were equally influential. Conclusion. Random and systematic environmental factors play a leading role in forming BP Var and CR in AH twins, with minimal role of hereditary factors

Determining the Efficiency of Cleaning A Milk Line Made From Different Materials From Contaminants

While moving along the milk-conducting systems in a milking machine, milk is in contact with the inner surface whose area exceeds 20 m2. That leads to the formation of protein-fat biofilms of contamination, which are a nutrient medium for the development of microorganisms. With insufficiently effective cleaning of these contaminants, in the periods between milking, the number of microflorae located in milk-conducting systems increases by tens of thousands of times. When cleaned with ineffective cleaning agents, mineral elements from milk are adsorbed on the surface of a protein-fat bio-film, which are subsequently compacted, changed, and converted into milk stone. In this case, the technical implementation of milk conducting systems is of critical importance. It has been established that a milk line made from any material is better cleaned with a hot washing solution than a cold one. Thus, with an increase in the temperature of a washing solution from 60 °C to 85 °C, the cleaning time of the milk line is reduced from 9.5 minutes to 1.5 minutes, or by 6 times. It was established that during the washing phase of a milk line there is a significant decrease in the temperature of the washing solution (≈30 %), which reduces the effectiveness of cleaning the parts of the system. Therefore, there is a need to maintain the solution temperature throughout the entire cleaning process. It is proved that the specific energy of adhesion of pollution in water is 2 times higher than that in a washing solution. With an increase in the temperature of the solution for every 10 °C, the decrease in the specific energy of pollution adhesion is on average 13 %. With an increase in the period after the end of milking before washing the milk line, the specific energy of its purification increases. The study reported here could lead improve the productivity of milking machines and the quality of the resulting product. That involves designing milking and dairy equipment from innovative materials