Cu-Enhanced 3-D Printed Fuels for Green SmallSat Propulsion

The Propulsion Research Laboratory at Utah State University (USU) has recently developed a promising High-Performance Green Hybrid Propulsion (HPGHP) technology that derives from the novel electrical breakdown property of certain 3-D printed like acrylonitrile butadiene styrene (ABS). This electrical breakdown property has been engineered into a proprietary, power-efficient system that can be cold-started and restarted with a high degree of reliability. One of the issues associated with ABS as a propellant is its low burn rate. It is well documented in technical literature that hybrid rocket systems generally have fuel regression rates that are typically 25-30% lower than solid fuel motors in the same thrust and impulse class. Lowered fuel regression rates tend to produce unacceptably low equivalence ratios that lead to poor mass-impulse performance, erosive fuel burning, nozzle erosion, reduced motor duty cycles, and the potential for combustion instability. To achieve equivalence ratios that produce acceptable combustion characteristics, hybrid fuel ports are often fabricated with large length-to-diameter ratios. The resulting poor volumetric efficiency is incompatible with Small Satellite (SmallSat) applications. This paper presents preliminary results from a collaborative development program between the University of Miami (UM) and USU. In this reported work, modern extrusion and 3-D printing techniques are used to fabricate sample ABS fuel grains with varying levels of copper-metallization. Hybrid-ABS fuel grains were printed from Cu-infused feed stock with 2%, 4%, and 6% Cu-mass concentrations. As baseline control, 100% pure ABS fuel grains (0% Cu) were also printed. Heat conduction via the additive copper (Cu) provides an efficient heat transfer mechanism that augments surface convection from the flame zone. Forced convection, the primary mechanism for pyrolysis for hybrid fuels, is generally inefficient due to wall-blowing associated with the radially emanating mass flow from fuel pyrolysis. Wall-blowing pushes the flame zone away from the fuel surface and significantly reduces the rate of enthalpy exchange. Homogeneously mixing a high conductivity metal such as Cu into the ABS fuel provides an efficient heat transfer mechanism, and allows radiant heat from the flame zone to be transferred deep into the fuel material. This process significantly increases the pyrolytic efficiency of the fuels. The Cu-infused fuels were tested at USU using a legacy 12-N hybrid thruster system. Fabrication and manufacturing methods are described, and results of hot fire tests are presented. The top-level conclusion is that Cu-infusion of the printed fuels measurably increases the fuel regression rate, allowing for a higher thrust level with no increase in the required volume. The Cu-infusion has negligible impact on the propellant characteristic velocity and the overall system specific impulse. The increased burn rate and overall increase in solid-fuel density resulting from Cu-infusion allows a measurable increase in the propellant impulse-density. This increase in volumetric efficiency is potentially significant for small spacecraft applications where available space has a premium value. Follow-on methods that infuse lower-molecular weight and higher thermal conductivity materials like graphene and carbon-nanotubes are proposed

PIP5KIβ Selectively Modulates Apical Endocytosis in Polarized Renal Epithelial Cells

Localized synthesis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] at clathrin coated pits (CCPs) is crucial for the recruitment of adaptors and other components of the internalization machinery, as well as for regulating actin dynamics during endocytosis. PtdIns(4,5)P2 is synthesized from phosphatidylinositol 4-phosphate by any of three phosphatidylinositol 5-kinase type I (PIP5KI) isoforms (α, β or γ). PIP5KIβ localizes almost exclusively to the apical surface in polarized mouse cortical collecting duct cells, whereas the other isoforms have a less polarized membrane distribution. We therefore investigated the role of PIP5KI isoforms in endocytosis at the apical and basolateral domains. Endocytosis at the apical surface is known to occur more slowly than at the basolateral surface. Apical endocytosis was selectively stimulated by overexpression of PIP5KIβ whereas the other isoforms had no effect on either apical or basolateral internalization. We found no difference in the affinity for PtdIns(4,5)P2-containing liposomes of the PtdIns(4,5)P2 binding domains of epsin and Dab2, consistent with a generic effect of elevated PtdIns(4,5)P2 on apical endocytosis. Additionally, using apical total internal reflection fluorescence imaging and electron microscopy we found that cells overexpressing PIP5KIβ have fewer apical CCPs but more internalized coated structures than control cells, consistent with enhanced maturation of apical CCPs. Together, our results suggest that synthesis of PtdIns(4,5)P2 mediated by PIP5KIβ is rate limiting for apical but not basolateral endocytosis in polarized kidney cells. PtdIns(4,5)P2 may be required to overcome specific structural constraints that limit the efficiency of apical endocytosis. © 2013 Szalinski et al

Idiopathic isolated clitoromegaly: A report of two cases

BACKGROUND: Clitoromegaly is a frequent congenital malformation, but acquired clitoral enlargement is relatively rare. METHODS: Two acquired clitoromegaly cases treated in Atatürk Training Hospital, Izmir, Turkey are presented. RESULTS: History from both patients revealed clitoromegaly over the last three years. Neither gynecological nor systemic abnormalities were detected in either patient. Karyotype analyses and hormonal tests were normal. Abdominal and gynaecological ultrasound did not show any cystic lesion or other abnormal finding. Computerized tomography scan of the adrenal glands was normal. Clitoroplasty with preservation of neurovascular pedicles was performed for the treatment of clitoromegaly. CONCLUSION: The patients were diagnosed as "idiopathic isolated" clitoromegaly. To the best of our knowledge, there has been no detailed report about idiopathic clitoromegaly in the literature

Quantitative metric profiles capture three-dimensional temporospatial architecture to discriminate cellular functional states

<p>Abstract</p> <p>Background</p> <p>Computational analysis of tissue structure reveals sub-visual differences in tissue functional states by extracting quantitative signature features that establish a diagnostic profile. Incomplete and/or inaccurate profiles contribute to misdiagnosis.</p> <p>Methods</p> <p>In order to create more complete tissue structure profiles, we adapted our cell-graph method for extracting quantitative features from histopathology images to now capture temporospatial traits of three-dimensional collagen hydrogel cell cultures. Cell-graphs were proposed to characterize the spatial organization between the cells in tissues by exploiting graph theory wherein the nuclei of the cells constitute the <it>nodes </it>and the approximate adjacency of cells are represented with <it>edges</it>. We chose 11 different cell types representing non-tumorigenic, pre-cancerous, and malignant states from multiple tissue origins.</p> <p>Results</p> <p>We built cell-graphs from the cellular hydrogel images and computed a large set of features describing the structural characteristics captured by the graphs over time. Using three-mode tensor analysis, we identified the five most significant features (metrics) that capture the compactness, clustering, and spatial uniformity of the 3D architectural changes for each cell type throughout the time course. Importantly, four of these metrics are also the discriminative features for our histopathology data from our previous studies.</p> <p>Conclusions</p> <p>Together, these descriptive metrics provide rigorous quantitative representations of image information that other image analysis methods do not. Examining the changes in these five metrics allowed us to easily discriminate between all 11 cell types, whereas differences from visual examination of the images are not as apparent. These results demonstrate that application of the cell-graph technique to 3D image data yields discriminative metrics that have the potential to improve the accuracy of image-based tissue profiles, and thus improve the detection and diagnosis of disease.</p

The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity

The Fc receptor FcRn traffics immunoglobulin G (IgG) in both directions across polarized epithelial cells that line mucosal surfaces, contributing to host defense. We show that FcRn traffics IgG from either apical or basolateral membranes into the recycling endosome (RE), after which the actin motor myosin Vb and the GTPase Rab25 regulate a sorting step that specifies transcytosis without affecting recycling. Another regulatory component of the RE, Rab11a, is dispensable for transcytosis, but regulates recycling to the basolateral membrane only. None of these proteins affect FcRn trafficking away from lysosomes. Thus, FcRn transcytotic and recycling sorting steps are distinct. These results are consistent with a single structurally and functionally heterogeneous RE compartment that traffics FcRn to both cell surfaces while discriminating between recycling and transcytosis pathways polarized in their direction of transport