A comparison of three systemic accident analysis methods using 46 SPAD (Signals Passed at Danger) incidents

During the period 1996-2003 there were five fatal accidents on the UK railway network, three of which were Signals Passed at Danger (SPAD) events (Watford Junction, 1996; Southall, 1997; Ladbroke Grove, 1999). SPAD events vary in severity and whilst most are not fatal there is the potential to cause serious injuries to passengers and train staff and damage to railway infra-structure. This paper investigates how the current system accident analysis tool used within the railway, the Incident Factor Classification System (IFCS) identifies and analyses causal factors of SPAD events. To evaluate the effectiveness IFCS was used to analysis SPAD incident reports (n=46) and the outputs were compared with two systemic accident analysis methods and relevant outputs (the Human Factors Analysis and Classification System – HFACS and Acci-Maps). The initial reporting process proved to hinder all systemic accident analysis methods in the extraction of causal factors. However, once extracted, all system accident analysis methods were successful in categorizing causal factors and demonstrated various outputs to illustrate the findings

“Tell them what they want to hear and get back to work”: insights into the utility of current occupational health assessments from the perspectives of train drivers

Australian train drivers undergo periodic health assessments as part of a nationally standardised approach to reducing sudden incapacitation risk, given the demonstrated potential for occupational and public harm. These assessments occur pre-placement, then every 5 years to age 50, then every 2 years to age 60, and then every year. Despite some reported benefits to rail workforce health indicators since implementation, research suggests the assessments are not operating as effectively as they might. For example, the prevalence of obesity in drivers is higher than in the general population and continues to increase. To improve this, there is a need to understand the experiences of drivers undergoing workplace health assessments. The aims of this study were to examine train drivers’ perceptions and experiences of the assessments, understand how these experiences shape their engagement with the process, and to generate recommendations for improvement from a systems thinking perspective. A qualitative design was used, involving semi-structured interviews within five focus groups of train drivers (n = 29) held across four Australian rail organisations. Questions addressed drivers’ backgrounds, their understanding of the National Standard, experiences of and attitudes towards health assessments, lifestyle risk factors, and personal approach to health and wellbeing. Transcript data were subjected to thematic analysis. Five factors were identified: drivers’ unmet information needs, perceived low reliability and validity of assessment, need for psychological wellbeing assessment and support, maladaptive threat avoidance strategies, and focus on short-term outcomes and compliance. The global theme was reactive organisational culture. Findings suggest that driver engagement with health assessment can be improved by proactively addressing the identified factors in occupational health initiatives and preventative interventions to tackle the burgeoning problem of train driver health impairment.Anjum Naweed, Janine Chapman, Joshua Trig

Quercetin Targets Cysteine String Protein (CSPα) and Impairs Synaptic Transmission

Cysteine string protein (CSPalpha) is a synaptic vesicle protein that displays unique anti-neurodegenerative properties. CSPalpha is a member of the conserved J protein family, also called the Hsp40 (heat shock protein of 40 kDa) protein family, whose importance in protein folding has been recognized for many years. Deletion of the CSPalpha in mice results in knockout mice that are normal for the first 2-3 weeks of life followed by an unexplained presynaptic neurodegeneration and premature death. How CSPalpha prevents neurodegeneration is currently not known. As a neuroprotective synaptic vesicle protein, CSPalpha represents a promising therapeutic target for the prevention of neurodegenerative disorders.Here, we demonstrate that the flavonoid quercetin promotes formation of stable CSPalpha-CSPalpha dimers and that quercetin-induced dimerization is dependent on the unique cysteine string region. Furthermore, in primary cultures of Lymnaea neurons, quercetin induction of CSPalpha dimers correlates with an inhibition of synapse formation and synaptic transmission suggesting that quercetin interfers with CSPalpha function. Quercetin's action on CSPalpha is concentration dependent and does not promote dimerization of other synaptic proteins or other J protein family members and reduces the assembly of CSPalpha:Hsc70 units (70kDa heat shock cognate protein).Quercetin is a plant derived flavonoid and popular nutritional supplement proposed to prevent memory loss and altitude sickness among other ailments, although its precise mechanism(s) of action has been unclear. In view of the therapeutic promise of upregulation of CSPalpha and the undesired consequences of CSPalpha dysfunction, our data establish an essential proof of principle that pharmaceutical agents can selectively target the neuroprotective J protein CSPalpha

From Understanding Cellular Function to Novel Drug Discovery: The Role of Planar Patch-Clamp Array Chip Technology

All excitable cell functions rely upon ion channels that are embedded in their plasma membrane. Perturbations of ion channel structure or function result in pathologies ranging from cardiac dysfunction to neurodegenerative disorders. Consequently, to understand the functions of excitable cells and to remedy their pathophysiology, it is important to understand the ion channel functions under various experimental conditions – including exposure to novel drug targets. Glass pipette patch-clamp is the state of the art technique to monitor the intrinsic and synaptic properties of neurons. However, this technique is labor intensive and has low data throughput. Planar patch-clamp chips, integrated into automated systems, offer high throughputs but are limited to isolated cells from suspensions, thus limiting their use in modeling physiological function. These chips are therefore not most suitable for studies involving neuronal communication. Multielectrode arrays (MEAs), in contrast, have the ability to monitor network activity by measuring local field potentials from multiple extracellular sites, but specific ion channel activity is challenging to extract from these multiplexed signals. Here we describe a novel planar patch-clamp chip technology that enables the simultaneous high-resolution electrophysiological interrogation of individual neurons at multiple sites in synaptically connected neuronal networks, thereby combining the advantages of MEA and patch-clamp techniques. Each neuron can be probed through an aperture that connects to a dedicated subterranean microfluidic channel. Neurons growing in networks are aligned to the apertures by physisorbed or chemisorbed chemical cues. In this review, we describe the design and fabrication process of these chips, approaches to chemical patterning for cell placement, and present physiological data from cultured neuronal cells

Реинжиниринг процессов в отделе экономики и финансов Красноярского ЦФТО с использованием BestPractices

The development of new types of sophisticated soft x-ray sources requires the knowledge of their emission characteristics such as photon flux, spectral distribution, and size of the radiation source. Calibrated spectrographs for the soft x-ray region are needed to determine these properties. The components of a soft x-ray spectrograph consisting of a pinhole gold transmission grating and a charge coupled device (CCD) camera are calibrated at the radiometry laboratory of the Physikalisch-Technische Bundesanstalt using the synchrotron radiation facility BESSY. Two different kinds of CCD-based photon detectors (one thinned and back illuminated, one coated with a phosphorous layer) are compared with regard to their sensitivities in the spectral range between 50 eV and 1.7 keV. The results obtained for the thinned CCD are compared with theoretical calculations of the sensitivity

Assessing the spectrum of germline variation in Fanconi anemia genes among patients with head and neck carcinoma before age 50

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138837/1/cncr30802.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/138837/2/cncr30802_am.pd

The heterogeneity of attenuated and brief limited psychotic symptoms: association of contents with age, sex, country, religion, comorbidities, and functioning

INTRODUCTION The Attenuated Psychosis Symptoms (APS) syndrome mostly represents the ultra-high-risk state of psychosis but, as does the Brief Intermittent Psychotic Symptoms (BIPS) syndrome, shows a large variance in conversion rates. This may be due to the heterogeneity of APS/BIPS that may be related to the effects of culture, sex, age, and other psychiatric morbidities. Thus, we investigated the different thematic contents of APS and their association with sex, age, country, religion, comorbidity, and functioning to gain a better understanding of the psychosis-risk syndrome. METHOD A sample of 232 clinical high-risk subjects according to the ultra-high risk and basic symptom criteria was recruited as part of a European study conducted in Germany, Italy, Switzerland, and Finland. Case vignettes, originally used for supervision of inclusion criteria, were investigated for APS/BIPS contents, which were compared for sex, age, country, religion, functioning, and comorbidities using chi-squared tests and regression analyses. RESULT We extracted 109 different contents, mainly of APS (96.8%): 63 delusional, 29 hallucinatory, and 17 speech-disorganized contents. Only 20 contents (18.3%) were present in at least 5% of the sample, with paranoid and referential ideas being the most frequent. Thirty-one (28.5%) contents, in particular, bizarre ideas and perceptual abnormalities, demonstrated an association with age, country, comorbidity, or functioning, with regression models of country and obsessive-compulsive disorders explaining most of the variance: 55.8 and 38.3%, respectively. Contents did not differ between religious groups. CONCLUSION Psychosis-risk patients report a wide range of different contents of APS/BIPS, underlining the psychopathological heterogeneity of this group but also revealing a potential core set of contents. Compared to earlier reports on North-American samples, our maximum prevalence rates of contents were considerably lower; this likely being related to a stricter rating of APS/BIPS and cultural influences, in particular, higher schizotypy reported in North-America. The various associations of some APS/BIPS contents with country, age, comorbidities, and functioning might moderate their clinical severity and, consequently, the related risk for psychosis and/or persistent functional disability