Modulational instability of bright solitary waves in incoherently coupled nonlinear Schr\"odinger equations

We present a detailed analysis of the modulational instability (MI) of ground-state bright solitary solutions of two incoherently coupled nonlinear Schr\"odinger equations. Varying the relative strength of cross-phase and self-phase effects we show existence and origin of four branches of MI of the two-wave solitary solutions. We give a physical interpretation of our results in terms of the group velocity dispersion (GVD) induced polarization dynamics of spatial solitary waves. In particular, we show that in media with normal GVD spatial symmetry breaking changes to polarization symmetry breaking when the relative strength of the cross-phase modulation exceeds a certain threshold value. The analytical and numerical stability analyses are fully supported by an extensive series of numerical simulations of the full model.Comment: Physical Review E, July, 199

The First Case of <I>Yersinia Pestis</I> Subsp. <I>Pestis</I> Isolation in the Territory of Altai Mountain Natural Plague Focus. Communication 1. Microbiological Characteristics, Molecular-Genetic and Mass-Spectrometric Identification of the Isolate

Performed is a complex microbiological, molecular-genetic and mass-spectrometric identification of Yersinia pestis main ssp. strain, which was isolated for the first time in the history of surveillance over the Altai mountain natural plague focus in June, 2012. Determined is its high universal virulence. Plasmid screening, multi-locus VNTR- and mass-spectrometric analyses have revealed the strain to be more closely related to the plague agent variant, circulating in the territory of the natural focus Khuukh-Serkh-Munkh-Khairkhan, Bayan-Ul’giisk aimak, Mongolia

Analysis of Epidemiological Situation on Hemorrhagic Fever with Renal Syndrome in the Khabarovsk Territory and the Jewish Autonomous Region, and Prognosis of Its Development for the Past-Flood Period in 2013-2014

Complex analysis of the data concerning epidemiological situation on HFRS in the Khabarovsk Territory and the Jewish Autonomous Region for the past decade (2003-2012) previous to high water in 2013 and results of operative epidemiological inspection of the flooding area and the adjoining territory where a high index of laboratory evidences of the virus presence in the main carriers was revealed, outlines high probability of the epidemiological condition complications in view of the infection in the post-flooding period in 2013-2014 in the both entities. Therewith on the basis of the unfavorable prognosis for the epidemiological situation development a Plan of organizational, preventive and anti-epidemic measures directed to minimization of risks related to aggravation of epidemiological situation on hemorrhagic fever with renal syndrome has been developed and is being implemented

A Giant Bipolar Shell around the WO star in the Galaxy IC 1613: Structure and Kinematics

Observations of the nebula associated with the WO star in the galaxy IC 1613 are presented. The observations were carried out with a scanning Interferometer Fabry-Perot in H_alpha at the 6m Special Astrophysical Observatory telescope; narrow-band H_alpha and [OIII] images were obtained with the 4-m KPNO telescope. The monochromatic H_alpha image clearly reveals a giant bipolar shell structure outside the bright nebula S3. The sizes of the southeastern and northwestern shells are 112x77 pc and (186-192)x(214-224) pc, respectively. We have studied the object's kinematics for the first time and found evidence for expansion of both shells. The expansion velocities of the southeastern and northwestern shells exceed 50 and 70 km/s, respectively. We revealed a filamentary structure of the shells and several compact features in the S3 core. A scenario is proposed for the formation of the giant bipolar structure by the stellar wind from the central WO star located at the boundary of a dense ``supercavity'' in the galaxy's H I distribution.Comment: 10 pages with 4 PS figure

Results of Immunological Screening for Natural-Focal and «Exotic» Infectious Diseases among Certain Population Groups of the Khabarovsk Territory, the Amur Region and the Jewish Autonomous Region

Displayed are the results of examination of immuno-competent local population of the Khabarovsk Territory, the Jewish Autonomous Region, the Amur Region, as well as foreign residents living and temporarily working in the areas, on a wide range of natural-focal bacterial and viral infectious diseases including the causative agents of some “exotic” infections too. Investigations have been carried out with the participation of experts from the specialized anti-epidemic team No. 1 (Irkutsk Research Anti-Plague Institute), who worked in the Amur Region, and a group of laboratory-epidemiological specialists from the team No. 2 - deployed in the Khabarovsk Territory and the Jewish Autonomous Region during the flooding in August-September 2013. The total of 1335 blood sera samples has been tested using serological methods. The findings have revealed the presence of immuno-competent population in the three regions of the Far Eastern Federal district in reference to the agents of natural-focal infectious diseases: tularemia, leptospirosis, yersinioses, hemorrhagic fever with renal syndrome, human granulocytic anaplasmosis, tick-borne borrelioses, tick-borne viral encephalitis, Californian encephalitis serogroup, Sindbis, West Nile and Dengue fevers. Circulation of Batai and Crimean-Congo hemorrhagic fever viruses has not been revealed based on serological assays

Analysis of Epidemiological Situation on Leptospiroses in the Amur-River Region. Operational Experience in the Flood Area in 2013, and Forecasting for 2014

For the first time ever mobile SAET units have performed complex investigation of epidemiological situation on leptospiroses in order to provide sanitary-epidemiological welfare of the population in the Amur-River region devastated by high water in 2013. Analyzed have been contemporary published and archival data on morbidity rate and epizootic activity of the natural leptospirosis foci in the Amur-River territory up to 2013. Summarized is the information concerning monitoring over natural focal infections (in particular leptospiroses) in the flooding area, obtained by specialized antiepidemic teams from Irkutsk Research Anti-Plague Institute of Siberia and Far East deployed in Belogorsk town of the Amur Region and Khabarovsk city in 2013. Epizootiological situation during the high water period in the Jewish Autonomous Region has been defined as an extremely adverse, unlike the Khabarovsk Territory and the Amur Region where epizootic process is less expressed. Therewith aggravation of epidemiological situation on leptospiroses in the Amur-River Region in 2014 is not ruled out

Infection of an Individual with Plague in the Gorno-Altaisk High-Mountain Natural Focus in 2014. Communication 2. Peculiarities of Laboratory Diagnostics and Molecular-Genetic Characterization of the Isolated Strains

Laboratory diagnostics of plague was carried out in compliance with valid operational guidelines and regulations. But its peculiarity consisted in the performance of diagnostic investigations secondary to antimicrobial therapy with application of preparations characterized by the expressed activity towards gram-negative microorganisms, including the agent of plague (ceftriaxone, ciprolet, and amikacin). The studies revealed that under antibiotic treatment during the early phase of infection the most effective method for the laboratory plague diagnostics was PCR. Based on the results of the assay it was possible to establish not only provisional, but also the final diagnosis in a patient. Obtained was genetic characteristics of the strains isolated from the patient and the marmot, withdrawn at the patient’s place, using techniques of molecular-genetic analysis, in particular PCR, multilocus VNTR, and multilocus and genome-wide sequencing. Thereupon the strains were attributed to antique biovar of the main subspecies of plague agent. In addition, close relation to Y. pestis of the main subspecies isolated in the same focus in 2012 and to the strains from Mongolian Altai and Tuvinian mountain focus was determined based on phylogenetic analysis of the isolates

Ассоциированные с полом и метаболическими нарушениями изменения в уровне матриксных металлопротеиназ в экзосомах при колоректальном раке

The objective is to evaluate the contents of matrix metalloproteinases (MMP) MMP9, MMP2, as well as their inducer EMMPRIN in circulating exosomes of patients with colorectal cancer in relation with clinical and morphological parameters, as well as with the presence of metabolic syndrome to search for promising exosomal markers associated with invasion, metastasis and metabolic disorders.Materials and methods. The study included 40 patients with colorectal cancer (T2–4N0–2M0–1) and 10 control patients. Exosomes of blood plasma were isolated by ultrafiltration with ultracentrifugation. The level of MMP9, MMP2 and their inducer EMMPRIN in exosomes was evaluated by flow cytometry.Results and conclusion. The level of MMP9‑positive exosomes was significantly higher in patients with colorectal cancer compared with patients with colorectal polyps. The proportion of MMP9‑negative and triple positive MMP9 + / MMP2+ / EMMPRIN+ exosomes, on the contrary, was higher in patients with polyps compared with patients with colorectal cancer. Mixed subpopulation of MMP9+ / MMP2– / EMMPRIN-exosomes prevailed both in patients with colorectal cancer and in control patients. There were no significant differences in the subpopulations of MMP and EMMPRIN in the exosomes of colorectal cancer patients depending on the age, stage, grade and localization. Gender differences in the occurrence of a triple-positive exosome subpopulation in colorectal cancer patients have been revealed. No relationship was found between the expression of MMP and EMMPRIN in exosomes and the presence of the metabolic syndrome, anthropometric parameters, the level of total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol. However, the relationships between MMP9+ / MMP2– / EMMPRIN–, MMP9+ / MMP2– / EMMPRIN– and the level of triglycerides and glucose in blood serum were revealed. Further studies are needed to study the characteristics of exosomes associated with metabolic disorders and the possibility of their use as diagnostic, prognostic, or predictor biomarkers.Цель исследования – оценить содержание матриксных металопротеиназ (ММР) ММР9 и ММР2, а также их индуктора EMMPRIN в циркулирующих экзосомах больных колоректальным раком (КРР) в связи с клиническо-морфологическими параметрами, а также с наличием метаболического синдрома для поиска перспективных экзосомальных маркеров, связанных с инвазией, метастазированием и метаболическими нарушениями.Материалы и методы. В исследование были включены 40 больных КРР (T2–4N0–2M0–1) и 10 пациентов контрольной группы. Экзосомы плазмы крови выделены методом ультрафильтрации с ультрацентрифугированием. Уровни ММР9, ММР2, а также их индуктора EMMPRIN оценивали с помощью проточной цитометрии.Результаты и заключение. Экспрессия ММР9 в образцах экзосом была значимо выше у больных КРР по сравнению с больными с колоректальными полипами. Доля ММР9‑отрицательных образцов латексных частиц, содержащих экзосомы, и образцов с положительной экспрессией ММР9 / ММР2 / EMMPRIN, наоборот, была выше у пациентов с полипами по сравнению с больными КРР. Образцы латексных частиц, содержащих экзосомы, экспрессирующие ММР9 и отрицательные по 2 другим маркерам, преобладали как у больных КРР, так и у пациентов контрольной группы. Не выявлено значимых различий в экспрессии ММР и EMMPRIN в образцах экзосом плазмы крови больных КРР в зависимости от возраста, стадии, степени дифференцировки и локализации опухолевого процесса. Выявлены гендерные различия в экспрессии ММР и EMMPRIN в образцах латексных частиц, содержащих экзосомы у больных КРР. Не обнаружено взаимосвязей экспрессии ММР и EMMPRIN в экзосомах и наличия метаболического синдрома, антропометрических параметров, уровней общего холестерина, холестерина липопротеинов низкой плотности и липопротеинов высокой плотности. Однако выявлены связи экспрессии образцов латексных частиц, содержащих экзосомы, экспрессирующих ММР9 и отрицательных по ММР2 и EMMPRIN (r = 0,51 и r = –0,56 соответственно; p &lt;0,05), а также экспрессирующих ММР9 и EMMPRIN и отрицательных по ММР2 (r = –0,60 и r = 0,57 соответственно; p &lt;0,05), с уровнями триглицеридов и глюкозы в сыворотке крови. Необходимы дальнейшие исследования по изучению характеристик экзосом, ассоциированных с метаболическими нарушениями, и возможности их использования в качестве диагностических, прогностических или предикторных маркеров

Выявление Bordetella holmesii среди больных, госпитализированных в стационар с подозрением на коклюш или коклюшеподобные заболевания

Purpose. To reveal and estimate prevalence of B. holmesii among the patients hospitalized with suspicion pertussis and pertussis-like illnesses.Materials and methods. 424 clinical samples received from patients with of pertussis and pertussis-like illnesses in GBUZ IKB № 1 DZM in 2017–2018 are investigated. Identification of fragments of a genome of Bordetella was carried out in PCR-RT with “Amplisens® Bordetella multi-FL”. For identification of fragments of a genome of B. holmesii used PCR-RT with primers of IS481, IS1001 and hIS1001.Results. The research included 424 patients, from them 56,1% of children aged till 1 year, 41,3% of children – are more senior than 1 year and 2,6% of adults. When using test system 60,4% of the samples containing DNA of B. pertussis are revealed; 1,9% of samples – DNA of B. parapertussis; in 34,9% of samples it is received negative and in 2,8% – doubtful results. The research of 424 samples in PCR-RT by means of IS481, IS1001 and hIS1001 primers showed that 61,1% of samples contained DNA of B. pertussis; 0,7% of samples – DNA of B. parapertussis and 3,8% of samples – DNA of B. holmesii. In 143 samples the result was negative. From 16 DNA of B. holmesii – positive samples, 9 samples were negative in test system earlier, in 2 samples – the doubtful result, 1 sample was earlier identified as DNA of B. parapertussis and in 4 samples DNA of B. pertussis and B. holmesii are found.Conclusion. The research demonstrates circulation of B. holmesii in the territory of Russia that is confirmed by identification of positive samples in 3,8% of cases among the sick children and adults hospitalized in a hospital with suspicion of pertussis and pertussis-like illnesses. For increase in efficiency of laboratory confirmation of the clinical diagnosis of pertussis and pertussis-like illnesses the genodiagnostic of a pertussis is recommended to improve taking into account identification DNA of B. holmesii.Цель: выявить и оценить распространенность B. holmesii среди больных, госпитализированных в стационар с подозрением на коклюш и коклюшеподобные заболевания.Материалы и методы. Исследовано 424 пробы клинического материала от больных с подозрением на коклюш и коклюшеподобные заболевания, госпитализированных в Инфекционную клиническую больницу № 1 в 2017– 2018 гг. Выявление фрагментов генома бордетелл осуществляли в ПЦР-РВ с «АмплиСенс® Bordetella multi-FL». Для идентификации фрагментов генома B. holmesii использовали ПЦР-РВ с праймерами IS481, IS1001 и hIS1001. Результаты. В исследование включено 424 пациента, из них 57,6% детей до 1 года, 42,3% детей старше 1 года и 2,6% взрослых. При использовании тестсистемы обнаружено 60,4% образцов, содержащих ДНК B. pertussis; 1,9% образцов – ДНК B. parapertussis; в 34,9% образцов получен отрицательный и в 2,8% – сомнительный результаты. Исследование 424 образцов в ПЦР-РВ с помощью IS481, IS1001 и hIS1001 праймеров показало, что 61,1% образцов содержали ДНК B. pertussis; 0,7% образцов – ДНК В. parapertussis и 3,8% образцов – ДНК B. holmesii. В 143 образцах результат был отрицательным. Из 16 ДНК B. holmesii, 9 образцов ранее были отрицательными, в 2 образцах – сомнительный результат и 1 образец был ранее идентифицирован как ДНК B. parapertussis, в 4 образцах обнаружена ДНК B. pertussis и B. holmesii.Заключение. Исследование свидетельствует о циркуляции B. holmesii на территории России, что под тверждается выявлением положительных образцов в 3,8% случаев среди больных детей и взрослых, госпитализированных в стационар с подозрением на коклюш и коклюшеподобные заболевания. Для повышения эффективности лабораторного подтверждения клинического диагноза коклюша и коклюшеподобных заболеваний рекомендуется совершенствовать генодиагностику коклюшной инфекции с учетом идентификации ДНК B. holmesii