Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification.

The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive

Strategy to avoid vascular injuries in revision total hip arthroplasty with intrapelvic implants

Aims: Our objective was describing an algorithm to identify and prevent vascular injury in patients with intrapelvic components. Methods: Patients were defined as at risk to vascular injuries when components or cement migrated 5 mm or more beyond the ilioischial line in any of the pelvic incidences (anteroposterior and Judet view). In those patients, a serial investigation was initiated by a CT angiography, followed by a vascular surgeon evaluation. The investigation proceeded if necessary. The main goal was to assure a safe tissue plane between the hardware and the vessels. Results: In ten at-risk patients undergoing revision hip arthroplasty and submitted to our algorithm, six were recognized as being high risk to vascular injury during surgery. In those six high-risk patients, a preventive preoperative stent was implanted before the orthopaedic procedure. Four patients needed a second reinforcing stent to protect and to maintain the vessel anatomy deformed by the intrapelvic implants. Conclusion: The evaluation algorithm was useful to avoid blood vessels injury during revision total hip arthroplasty in high-risk patients

Avaliação do sistema comercial CandiFast para identificação de leveduras isoladas do mosto de uvas brancas e tintas.

A região do Vale dos Vinhedos, localizada em Bento Gonçalves - RS, possui indicação geográfica para elaboração de vinhos no Sul do Brasil, o que torna de extrema importância a seleção e a identificação de linhagens autóctones para elaboração de vinhos de qualidade.Resumo

INATIVAÇÃO DE ENZIMAS PELO MÉTODO DE BRANQUEAMENTO

Branquear um alimento é uma técnica de pré-preparo que consiste em provocar um choque térmico nos alimentos em um curto período de tempo de aplicação. Esta técnica facilita muito o trabalho da indústria de alimentos, auxiliando principalmente na conservação de um produto que após entrar em contato com o oxigênio perderia suas características sensoriais e nutricionais. Dentre suas utilidades destacam-se a inativação de enzimas causadoras do escurecimento,fixação da cor, aroma e sabor da fruta, eliminação de ar dos tecidos, evitar oxidações, deixar a consistência da fruta firme e tenra, redução da carga microbiana superficial, e o aumento da qualidade e vida de prateleira do vegetal

Seismic response analysis of multiple-frame bridges with unseating restrainers considering ground motion spatial variation and SSI

Unseating damages of bridge decks have been observed in many previous major earthquakes due to large relative displacement exceeding the available seat length. Steel cable restrainers are often used to limit such relative displacements. Present restrainer design methods are based on the relative displacements caused by the different dynamic characteristics of adjacent bridge structures. However, the relative displacements in bridge structures are not only caused by different dynamic characteristics of adjacent bridge segments. Recent studies indicated that differential ground motions at supports of bridge piers and Soil Structure Interaction (SSI) could have a significant influence on the relative displacement of adjacent bridge components. Thus the present design methods could significantly underestimate the relative displacement responses of the adjacent bridge components and the stiffness of the restrainers required to limit these displacements. None of the previous investigations considered the effects of spatially varying ground motions in evaluating the adequacy of the restrainers design methods. Moreover, the code recommendation of adjusting the fundamental frequencies of adjacent bridge structures close to each other to mitigate relative displacement induced damages is developed based on the uniform ground motion assumption. Investigations on its effectiveness to mitigate the relative displacement induced damages on the bridge structures subjected to spatially varying ground motion and SSI are made. This paper discusses the effects of spatially varying ground motions and SSI on the responses of the multiple-frame bridges with unseating restrainers through inelastic bridge response analysis

Inflammatory cytokines and biofilm production sustain Staphylococcus aureus outgrowth and persistence: A pivotal interplay in the pathogenesis of Atopic Dermatitis

Individuals with Atopic dermatitis (AD) are highly susceptible to Staphylococcus aureus colonization. However, the mechanisms driving this process as well as the impact of S. aureus in AD pathogenesis are still incompletely understood. In this study, we analysed the role of biofilm in sustaining S. aureus chronic persistence and its impact on AD severity. Further we explored whether key inflammatory cytokines overexpressed in AD might provide a selective advantage to S. aureus. Results show that the strength of biofilm production by S. aureus correlated with the severity of the skin lesion, being significantly higher (P < 0.01) in patients with a more severe form of the disease as compared to those individuals with mild AD. Additionally, interleukin (IL)-β and interferon γ (IFN-γ), but not interleukin (IL)-6, induced a concentration-dependent increase of S. aureus growth. This effect was not observed with coagulase-negative staphylococci isolated from the skin of AD patients. These findings indicate that inflammatory cytokines such as IL1-β and IFN-γ, can selectively promote S. aureus outgrowth, thus subverting the composition of the healthy skin microbiome. Moreover, biofilm production by S. aureus plays a relevant role in further supporting chronic colonization and disease severity, while providing an increased tolerance to antimicrobials

Mortality among patients with tuberculosis requiring intensive care: a retrospective cohort study

<p>Abstract</p> <p>Background</p> <p>To describe the characteristics of patients with tuberculosis (TB) requiring intensive care and to identify the factors that predicts in-hospital mortality in a city of a developing country with intermediate-to-high TB endemicity.</p> <p>Methods</p> <p>We conducted a retrospective, cohort study, between November 2005 and November 2007. The patients with TB requiring intensive care were included. Predictors of mortality were assessed. The primary outcome was the in-hospital mortality.</p> <p>Results</p> <p>During the study period, 67 patients with TB required intensive care. Of them, 62 (92.5%) had acute respiratory failure and required mechanical ventilation. Forty-four (65.7%) patients died. Coinfection with human immunodeficiency virus was present in 46 (68.7%) patients. Early intensive care unit admission and ventilator-associated pneumonia were independently associated with the in-hospital mortality.</p> <p>Conclusions</p> <p>In this study we found a high mortality rate in TB patients requiring intensive care, especially in those with an early ICU admission.</p

Identification of Brucella by MALDI-TOF Mass Spectrometry. Fast and Reliable Identification from Agar Plates and Blood Cultures

BACKGROUND: MALDI-TOF mass spectrometry (MS) is a reliable method for bacteria identification. Some databases used for this purpose lack reference profiles for Brucella species, which is still an important pathogen in wide areas around the world. We report the creation of profiles for MALDI-TOF Biotyper 2.0 database (Bruker Daltonics, Germany) and their usefulness for identifying brucellae from culture plates and blood cultures. METHODOLOGY/PRINCIPAL FINDINGS: We created MALDI Biotyper 2.0 profiles for type strains belonging to B. melitensis biotypes 1, 2 and 3; B. abortus biotypes 1, 2, 5 and 9; B. suis, B. canis, B ceti and B. pinnipedialis. Then, 131 clinical isolates grown on plate cultures were used in triplicate to check identification. Identification at genus level was always correct, although in most cases the three replicates reported different identification at species level. Simulated blood cultures were performed with type strains belonging to the main human pathogenic species (B. melitensis, B. abortus, B. suis and B. canis), and studied by MALDI-TOF MS in triplicate. Identification at genus level was always correct. CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS is reliable for Brucella identification to the genus level from culture plates and directly from blood culture bottles