EURAMET key comparison between INRiM and UME in Vickers hardness scales (HV1 - HV30) - EURAMET.M.H-K1.b and c

This report describes the method and results of a bilateral EURAMET Key Comparison in Vickers hardness scales of two National Metrology Institutes (NMIs) of Italy and Turkey, INRiM and UME, respectively. The Pilot Laboratory (PL) is INRiM in the comparison in which one set of hardness reference blocks with three hardness levels for the Vickers Hardness scales of both HV1 and HV30 was used. The comparison was realized as planned in the Technical Protocol with some delay. The aim of this comparison is to link the UME measurement results to the CCM.H-K1.b.c through the PL (INRiM) as a participant of the CCM key comparison. The measurement results and uncertainty assessments declared by INRiM and UME are in consistency with each other and UME results are also in consistency with the CCM.H-K1.b.c Key Comparison Reference Values (KCRVs). The CCM.H-K1.b.c was realized during 2001 to 2003 to investigate the metrological equivalence of national standards among national metrology institutes (NMIs) within the CCM

Result analysis of EURAMET Vickers comparison between INRiM and UME (EURAMET.M.H-K1.b.c)

A bilateral key comparison between INRiM (National Metrology Institute of Italy) and TUBITAK UME (National Metrology Institute of Turkey) had been decided to be organized in the field of Hardness Metrology to determine the consistency of the national hardness standards in both countries realizing Vickers Hardness measurements in accordance with ISO 6507–1:2018 [1] and ISO 6507–3:2018 [2] standards. Widely used Vickers Hardness scales such as HV1 and HV30 constitute the scope of the comparison which was piloted by INRiM. In this paper the procedure and measurement results of the bilateral EURAMET key comparison between the two laboratories are explained

Preliminary results of EURAMET Rockwell comparison between INRiM and UME (EURAMET.M.H-S1.A.B.C)

A bilateral supplementary comparison between INRiM (National Metrology Institute of Italy) and UME (National Metrology Institute of Turkey) had been decided to be organized in the field of Hardness Metrology to determine the consistency of the national hardness standards in both countries realizing Rockwell Hardness measurements in accordance with ISO 6508-1:2016 [1] and ISO 6508-3:2015 [2] standards. In this paper the procedure and preliminary measurement results of the bilateral EURAMET supplementary comparison between the two laboratories are explained

A Comparison of Blocking Methods for Record Linkage

Record linkage seeks to merge databases and to remove duplicates when unique identifiers are not available. Most approaches use blocking techniques to reduce the computational complexity associated with record linkage. We review traditional blocking techniques, which typically partition the records according to a set of field attributes, and consider two variants of a method known as locality sensitive hashing, sometimes referred to as "private blocking." We compare these approaches in terms of their recall, reduction ratio, and computational complexity. We evaluate these methods using different synthetic datafiles and conclude with a discussion of privacy-related issues.Comment: 22 pages, 2 tables, 7 figure

Result analysis of EURAMET Brinell comparison between INRiM, UME and PTB (EURAMET.M.H–S2.A.B)

A EURAMET supplementary comparison between INRiM (National Metrology Institute of Italy), UME (National Metrology Institute of Republic of Turkey) and PTB (National Metrology Institute of Germany) had been decided to be organized in the field of Hardness Metrology to determine the consistency of the national hardness standards in these three countries realizing Brinell Hardness measurements in accordance with ISO 6506–1:2014 [1] and ISO 6506–3:2014 [2] standards. Widely used Brinell Hardness scales such as HBW 1/30 and HBW 2.5/187.5 constitute the scope of the comparison. In this paper the procedure and comparison results are explained

Histone locus regulation by the Drosophila dosage compensation adaptor protein CLAMP

The conserved histone locus body (HLB) assembles prior to zygotic gene activation early during development and concentrates factors into a nuclear domain of coordinated histone gene regulation. Although HLBs form specifically at replication-dependent histone loci, the cis and trans factors that target HLB components to histone genes remained unknown. Here we report that conserved GA repeat cis elements within the bidirectional histone3–histone4 promoter direct HLB formation in Drosophila. In addition, the CLAMP (chromatin-linked adaptor for male-specific lethal [MSL] proteins) zinc finger protein binds these GA repeat motifs, increases chromatin accessibility, enhances histone gene transcription, and promotes HLB formation. We demonstrated previously that CLAMP also promotes the formation of another domain of coordinated gene regulation: the dosage-compensated male X chromosome. Therefore, CLAMP binding to GA repeat motifs promotes the formation of two distinct domains of coordinated gene activation located at different places in the genome

Management of Sigmoid Volvulus Avoiding Sigmoid Resection

Acute sigmoid volvulus is typically caused by an excessively mobile and redundant segment of colon with a stretched mesenteric pedicle. When this segment twists on its pedicle, the result can be obstruction, ischemia and perforation. A healthy, 18-year-old Caucasian woman presented to the emergency department complaining of cramping abdominal pain, distention, constipation and obstipation for the last 72 h, accompanied by nausea, vomiting and abdominal tenderness. The patient had tympanitic percussion tones and no bowel sounds. She was diagnosed with acute sigmoid volvulus. Although urgent resective surgery seems to be the appropriate treatment for those who present with acute abdominal pain, intestinal perforation or ischemic necrosis of the intestinal mucosa, the first therapeutic choice for clinically stable patients in good general condition is considered, by many institutions, to be endoscopic decompression. Controversy exists on the decision of the time, the type of definitive treatment, the strategy and the most appropriate surgical technique, especially for teenagers for whom sigmoid resection can be avoided

Specialized dynamical properties of promiscuous residues revealed by simulated conformational ensembles

The ability to interact with different partners is one of the most important features in proteins. Proteins that bind a large number of partners (hubs) have been often associated with intrinsic disorder. However, many examples exist of hubs with an ordered structure, and evidence of a general mechanism promoting promiscuity in ordered proteins is still elusive. An intriguing hypothesis is that promiscuous binding sites have specific dynamical properties, distinct from the rest of the interface and pre-existing in the protein isolated state. Here, we present the first comprehensive study of the intrinsic dynamics of promiscuous residues in a large protein data set. Different computational methods, from coarse-grained elastic models to geometry-based sampling methods and to full-atom Molecular Dynamics simulations, were used to generate conformational ensembles for the isolated proteins. The flexibility and dynamic correlations of interface residues with a different degree of binding promiscuity were calculated and compared considering side chain and backbone motions, the latter both on a local and on a global scale. The study revealed that (a) promiscuous residues tend to be more flexible than nonpromiscuous ones, (b) this additional flexibility has a higher degree of organization, and (c) evolutionary conservation and binding promiscuity have opposite effects on intrinsic dynamics. Findings on simulated ensembles were also validated on ensembles of experimental structures extracted from the Protein Data Bank (PDB). Additionally, the low occurrence of single nucleotide polymorphisms observed for promiscuous residues indicated a tendency to preserve binding diversity at these positions. A case study on two ubiquitin-like proteins exemplifies how binding promiscuity in evolutionary related proteins can be modulated by the fine-tuning of the interface dynamics. The interplay between promiscuity and flexibility highlighted here can inspire new directions in protein-protein interaction prediction and design methods. © 2013 American Chemical Society

Compounds from <em>Terminalia mantaly</em> L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against some Pathogenic Yeasts and Enzymes of Metabolic Significance<strong></strong>

Tchuenmogne MAT, Ngouana TK, Gohlke S, et al. Compounds from &lt;em&gt;Terminalia mantaly&lt;/em&gt; L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against some Pathogenic Yeasts and Enzymes of Metabolic Significance&lt;strong&gt;&lt;/strong&gt;. Preprints. 2016.The chemical investigation of the anti-yeast methanol extract from the stem bark of Terminalia mantaly led to the isolation of seven compounds: 3-O-methyl-4-O-&amp;alpha;-rhamnopyranoside ellagic acid (1), 3-O-mehylellagic acid (2), arjungenin or 2,3,19,23-tetrahydroxyolean-12-en-28-o&amp;iuml;c acid (3), arjunglucoside or 2,3,19,23-tetrahydroxyolean-12-en-28-o&amp;iuml;c acid glucopyranoside (4), 2&amp;alpha;,3&amp;alpha;,24-trihydroxyolean-11,13(18)-dien-28-o&amp;iuml;c acid (5), stigmasterol (6), stigmasterol 3-O-&amp;beta;-D-glucopyranoside (7). Their structures were established by means of spectroscopic analysis and comparison with published data. Compounds 1-5 were tested in vitro for activity against three pathogenic yeast isolates, Candida albicans, Candida parapsilosis and Candida krusei. The activity of compounds 1, 2 and 4 were comparable to that of the reference compound fluconazole (MIC values below 32 &amp;micro;g/ml) against the three tested yeast isolates. They were also tested for inhibitory properties against four enzymes of metabolic significance: Glucose-6-Phosphate Deshydrogenase (G6PD), human erythrocyte Carbonic anhydrase I and II (hCA I and hCA II), Glutathione S-transferase (GST). Compound 4 showed highly potent inhibitory property against the four tested enzymes with overall IC50 values below 4 &amp;micro;M and inhibitory constant (Ki) &amp;lt;3 &amp;micro;M.</jats:p