A novel oligoribonuclease of Escherichia coli. I. Isolation and properties

A new ribonuclease has been isolated from Escherichia coli. The enzyme is present in the 100,000 times g supernatant fraction and has been purified over 200-fold. Studies of the enzyme reveal that: 1. The enzyme shows a marked preference for oligoribonucleotides; indeed, the reaction rate is inversely proportional to the chain length of the substrate. The enzyme does not attack polynucleotides even at high concentrations of enzyme and has no detectable DNase activity. 2. The enzyme is stimulated strongly by Mn2+, less strongly by Mg2+, and not at all by Ca2+ and monovalent cations. 3. The enzyme is purified free of RNase I, RNase II, RNase III, polynucleotide phosphorylase, and other known ribonucleases of E. coli. The enzyme displays identical properties when isolated from mutants of E. coli that are deficient in the above ribonucleases. 4. The enzyme has a marked thermostability, a point of further distinction from RNase II

Vibronic mixing enables ultrafast energy flow in light-harvesting complex II.

Since the discovery of quantum beats in the two-dimensional electronic spectra of photosynthetic pigment-protein complexes over a decade ago, the origin and mechanistic function of these beats in photosynthetic light-harvesting has been extensively debated. The current consensus is that these long-lived oscillatory features likely result from electronic-vibrational mixing, however, it remains uncertain if such mixing significantly influences energy transport. Here, we examine the interplay between the electronic and nuclear degrees of freedom (DoF) during the excitation energy transfer (EET) dynamics of light-harvesting complex II (LHCII) with two-dimensional electronic-vibrational spectroscopy. Particularly, we show the involvement of the nuclear DoF during EET through the participation of higher-lying vibronic chlorophyll states and assign observed oscillatory features to specific EET pathways, demonstrating a significant step in mapping evolution from energy to physical space. These frequencies correspond to known vibrational modes of chlorophyll, suggesting that electronic-vibrational mixing facilitates rapid EET over moderately size energy gaps

Latent heat flux and canopy conductance based on Penman-Monteith and Bouchet’s complementary hypothesis:validation over diverse biomes

Abstract A novel method is presented to analytically resolve the terrestrial latent heat flux (λE) and conductances (boundary layer gB and surface gS) using net radiation (RN), ground heat flux (G), air temperature (Ta), and relative humidity (RH). This method consists of set of equations where the two unknown internal state variables (gB and gS) were expressed in terms of the known core variables, combining diffusion equations, the Penman–Monteith equation, the Priestley–Taylor equation, and Bouchet’s complementary hypothesis. Estimated λE is validated with the independent eddy covariance λE observations over Soil Moisture Experiment 2002 (SMEX-02); the Global Energy and Water Cycle Experiment (GEWEX) Continental-Scale International Project (GCIP) selected sites from FLUXNET and tropics eddy flux, representing four climate zones (tropics, subtropics, temperate, and cold); and multiple biomes. The authors find a RMSE of 23.8–54.6 W m−2 for hourly λE over SMEX-02 and GCIP and 23.8–29.0 W m−2 for monthly λE over the FLUXNET and tropics. Observational and modeled evidence in the reduction in annual evaporation (E) pattern on the order of 33% from 1999 to 2006 was found in central Amazonia. Retrieved gS responded to vapor pressure deficit, measured λE, and gross photosynthesis in a theoretically robust behavior. However, the current scheme [Penman–Monteith–Bouchet–Lhomme (PMBL)] showed some overestimation of λE in limited soil moisture regimes. PMBL provides similar results when compared with another Priestley–Taylor–based λE estimation approach [Priestley–Taylor–Jet Propulsion Laboratory (PT-JPL)] but with the advantage of having the conductances analytically recovered.</jats:p

Roles of atmospheric and land surface data in dynamic regional downscaling

In studies dealing with the impact of land use changes on atmospheric processes, a key methodological step is the validation of simulated current conditions. However, regions lacking detailed atmospheric and land use data provide limited information with which to accurately generate control simulations. In this situation, the difference between baseline control simulations and different land use change simulations can be quite different owing to the quality of the atmospheric and land use data sets. Using multiple simulations at the Monteverde cloud forest region of Costa Rica as an example, we show that when a regional climate model is used to study the effect of land use change, it can produce distinctly different results at regional scales, depending on the amount of data available to run the climate simulations. We show that for the specific case of land use change impact studies, the simulation results are very sensitive to the prescribed atmospheric information (e.g., lateral boundary conditions) compared to the land use (surface boundary) information

Cloudbursts in Indian Himalayas: a review

Cloudbursts in and around the southern rim of the Indian Himalayas are elusive in terms of their position and time of occurrences. Most of the reported cloudbursts are in the interior of the Himalayas and hence their observation itself is limited. Most of these events are reported once their affect in terms of loss to life and property is experienced in the downstream habitats. In addition, they are mostly associated with flash floods as an impact of the torrential precipitation. The principal understanding of the cloudburst is associated with sudden heavy deluge of precipitation in very less time interval over a very small area. Except this understanding and India Meteorology Department (IMD) definition of > 100 mm/h precipitation over a geographical region of approximately 20–30 km2, nothing much else is known about these events. There are a very few studies carried out on understanding of these events. Present paper synthesizes the available information and research on cloudburst events and tries to define it based on associated dynamics, thermodynamics and physical processes leading to a cloudburst event. Thus in the present work, characterizations and impacts of cloudburst leading from precipitation to dynamical to thermodynamical to large scale forcings to orographical forcings to followed geomorphology to impacts are intertwined to present comprehensive portray of it. Most of the cloudburst events are seen occurring in the elevation range of 1000 m to 2500 m within the valley folds of the southern rim of the Indian Himalayas. Apart from some of the large scale flow shown by few of the studies, it is found that cloudburst events are convectively triggered followed by orographically locked systems. These intertwined mechanisms lead cloudburst events to form. Amiss of any one of these mechanisms will not lead the cloudburst mechanism to form. These interactions in the present paper established the vagaries associated with the cloudburst events

Rayleigh Imaging of Graphene and Graphene Layers

We investigate graphene and graphene layers on different substrates by monochromatic and white-light confocal Rayleigh scattering microscopy. The image contrast depends sensitively on the dielectric properties of the sample as well as the substrate geometry and can be described quantitatively using the complex refractive index of bulk graphite. For few layers (<6) the monochromatic contrast increases linearly with thickness: the samples behave as a superposition of single sheets which act as independent two dimensional electron gases. Thus, Rayleigh imaging is a general, simple and quick tool to identify graphene layers, that is readily combined with Raman scattering, which provides structural identification.Comment: 8 pages, 9 figure

Effect of acute copper sulfate exposure on olfactory responses to amino acids and pheromones in goldfish (Carassius auratus)

Exposure of olfactory epithelium to environmentally relevant concentrations of copper disrupts olfaction in fish. To examine the dynamics of recovery at both functional and morphological levels after acute copper exposure, unilateral exposure of goldfish olfactory epithelia to 100 μM CuSO4 (10 min) was followed by electro-olfactogram (EOG) recording and scanning electron microscopy. Sensitivity to amino acids (L-arginine and L-serine), generally considered food-related odorants, recovered most rapidly (three days), followed by that to catecholamines(3-O-methoxytyramine),bileacids(taurolithocholic acid) and the steroid pheromone, 17,20 -dihydroxy-4-pregnen- 3-one 20-sulfate, which took 28 days to reach full recovery. Sensitivity to the postovulatory pheromone prostaglandin F2R had not fully recovered even at 28 days. These changes in sensitivity were correlated with changes in the recovery of ciliated and microvillous receptor cell types. Microvillous cells appeared largely unaffected by CuSO4 treatment. Cilia in ciliated receptor neurones, however, appeared damaged one day post-treatment and were virtually absent after three days but had begun to recover after 14 days. Together, these results support the hypothesis that microvillous receptor neurones detect amino acids whereas ciliated receptor neurones were not functional and are responsible for detection of social stimuli (bile acidsandpheromones).Furthermore, differences in sensitivity to copper may be due to different transduction pathways in the different cell types

The Unique Origin of Colors of Armchair Carbon Nanotubes

The colors of suspended metallic colloidal particles are determined by their size-dependent plasma resonance, while those of semiconducting colloidal particles are determined by their size-dependent band gap. Here, we present a novel case for armchair carbon nanotubes, suspended in aqueous medium, for which the color depends on their size-dependent excitonic resonance, even though the individual particles are metallic. We observe distinct colors of a series of armchair-enriched nanotube suspensions, highlighting the unique coloration mechanism of these one-dimensional metals.Comment: 4 pages, 3 figure

Why some stems are red: cauline anthocyanins shield photosystem II against high light stress

Red-stemmed plants are extremely common, yet the functions of cauline anthocyanins are largely unknown. The possibility that photoabatement by anthocyanins in the periderm reduces the propensity for photoinhibition in cortical chlorenchyma was tested for Cornus stolonifera. Anthocyanins were induced in green stems exposed to full sunlight. PSII quantum yields (ФPSII) and photochemical quenching coefficients were depressed less in red than in green stems, both under a light ramp and after prolonged exposures to saturating white light. These differences were primarily attributable to the attenuation of PAR, especially green/yellow light, by anthocyanins. However, the red internodes also had less chlorophyll and higher carotenoid:chlorophyll ratios than the green, and when the anthocyanic periderm was removed, small differences in the ФPSII of the underlying chlorenchyma were retained. Thus, light screening by cauline anthocyanins is important, but is only part of a set of protective acclimations to high irradiance. Hourly measurements of ФPSII on established trees under natural daylight indicated a possible advantage of red versus green stems under sub-saturating diffuse, but not direct sunlight. To judge the wider applicability of the hypothesis, responses to high light were compared for red and green stems across five further unrelated species. There was a strong, linear, interspecific correlation between photoprotective advantage and anthocyanin concentration differences among red and green internodes. The photoprotective effect appears to be a widespread phenomenon

Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses.

Drought is the most important environmental stress limiting crop yields. The C4 cereal sorghum [Sorghum bicolor (L.) Moench] is a critical food, forage, and emerging bioenergy crop that is notably drought-tolerant. We conducted a large-scale field experiment, imposing preflowering and postflowering drought stress on 2 genotypes of sorghum across a tightly resolved time series, from plant emergence to postanthesis, resulting in a dataset of nearly 400 transcriptomes. We observed a fast and global transcriptomic response in leaf and root tissues with clear temporal patterns, including modulation of well-known drought pathways. We also identified genotypic differences in core photosynthesis and reactive oxygen species scavenging pathways, highlighting possible mechanisms of drought tolerance and of the delayed senescence, characteristic of the stay-green phenotype. Finally, we discovered a large-scale depletion in the expression of genes critical to arbuscular mycorrhizal (AM) symbiosis, with a corresponding drop in AM fungal mass in the plants' roots