Improvement of the Wastewater Biodegradability by Means of Photocatalytic and Wet Oxidation Processes in the Presence of Hydrogen Peroxide

In this study, the effectiveness of photocatalytic oxidation (PO) and wet oxidation (WO) processes as a pre-treatment step on improvement of biodegradability and colour removal of mixture of raw domestic and pre-treated industrial wastewaters, have been evaluated. More oxygen was obtained by H2O2 (as an oxidant in WO and PO processes) than stoichiometric demand. PO of the wastewater was carried out by illumination of the wastewater with UV lamp (at room temperature, atmospheric pressure, 16.5 h reaction time) and WO of the wastewater was carried out by means of thermal oxidation at autoclave conditions (at 118–120 oC, 1.9–2 bar, 3 h reaction time). 1 g L–1 TiO2 in PO process and 0.2 mg L–1 Cu++ in WO process were used as catalyst. The results obtained from experiments were not compared with each other due to the difference between the quality and quantity of the used catalyst and the consumed energy. Colour removal efficiency was 33 % for WO process and 77.6 % for PO process. By applying WO process, 72.7 % increase in the reaction rate coefficient describing the degradability of organic compounds in wastewater was obtained, but this value was 34.5 % in PO process

Microdevices for Continuous Sized Based Sorting by AC Dielectrophoresis

This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.In this project, various microfluidic devices are designed for microparticles and cells separation. The dominant regions of dielectrophoresis among other AC Electrokinetic transport mechanisms are predicted. Flow behaviors of particles for each design parameter are modeled and simulated using COMSOL Multiphysics 4.3a software. Lab-on-a-chip devices having a Ti interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to investigate the most effective design solution for separating particles based on their sizes. Polystyrene particles with different diameters of 3.2 μm and 9.8 μm are used in our experiments and experiments with circulating tumor cells (CTCs) are in progress

Astrocyte - neuron lactate shuttle may boost more ATP supply to the neuron under hypoxic conditions - in silico study supported by in vitro expression data

<p>Abstract</p> <p>Background</p> <p>Neuro-glial interactions are important for normal functioning of the brain as well as brain energy metabolism. There are two major working models - in the classical view, both neurons and astrocytes can utilize glucose as the energy source through oxidative metabolism, whereas in the astrocyte-neuron lactate shuttle hypothesis (ANLSH) it is the astrocyte which can consume glucose through anaerobic glycolysis to pyruvate and then to lactate, and this lactate is secreted to the extracellular space to be taken up by the neuron for further oxidative degradation.</p> <p>Results</p> <p>In this computational study, we have included hypoxia-induced genetic regulation of these enzymes and transporters, and analyzed whether the ANLSH model can provide an advantage to either cell type in terms of supplying the energy demand. We have based this module on our own experimental analysis of hypoxia-dependent regulation of transcription of key metabolic enzymes. Using this experimentation-supported <it>in silico </it>modeling, we show that under both normoxic and hypoxic conditions in a given time period ANLSH model does indeed provide the neuron with more ATP than in the classical view.</p> <p>Conclusions</p> <p>Although the ANLSH is energetically more favorable for the neuron, it is not the case for the astrocyte in the long term. Considering the fact that astrocytes are more resilient to hypoxia, we would propose that there is likely a switch between the two models, based on the energy demand of the neuron, so as to maintain the survival of the neuron under hypoxic or glucose-and-oxygen-deprived conditions.</p

Apparent stress-strain relationships in experimental equipment where magnetorheological fluids operate under compression mode

Abstract: This paper presents an experimental investigation of two different magnetorheological ( MR) fluids, namely, water-based and hydrocarbon-based MR fluids in compression mode under various applied currents. Finite element method magnetics was used to predict the magnetic field distribution inside the MR fluids generated by a coil. A test rig was constructed where the MR fluid was sandwiched between two flat surfaces. During the compression, the upper surface was moved towards the lower surface in a vertical direction. Stress-strain relationships were obtained for arrangements of equipment where each type of fluid was involved, using compression test equipment. The apparent compressive stress was found to be increased with the increase in magnetic field strength. In addition, the apparent compressive stress of the water-based MR fluid showed a response to the compressive strain of greater magnitude. However, during the compression process, the hydrocarbon-based MR fluid appeared to show a unique behaviour where an abrupt pressure drop was discovered in a region where the apparent compressive stress would be expected to increase steadily. The conclusion is drawn that the apparent compressive stress of MR fluids is influenced strongly by the nature of the carrier fluid and by the magnitude of the applied current

Dendritic Cell Cross-Priming Is Essential for Immune Responses to Listeria monocytogenes

Cross-presentation is now recognized as a major mechanism for initiating CD8 T cell responses to virus and tumor antigens in vivo. It provides an elegant mechanism that allows relatively few Dendritic cells (DCs) to initiate primary immune responses while avoiding the consumptive nature of pathogenic infection. CD8 T cells play a major role in anti-bacterial immune responses; however, the contribution of cross-presentation for priming CD8 T cell responses to bacteria, in vivo, is not well established. Listeria monocytogenes (Listeria) is the causative agent of Listeriosis, an opportunistic food-borne bacterial infection that poses a significant public health risk. Here, we employ a transgenic mouse model in which cross-presentation is uniquely inactivated, to investigate cross-priming during primary Listeria infection. We show that cross-priming deficient mice are severely compromised in their ability to generate antigen-specific T cells to stimulate MHC I-restricted CTL responses following Listeria infection. The defect in generation of Listeria-elicited CD8 T cell responses is also apparent in vitro. However, in this setting, the endogenous route of processing Listeria-derived antigens is predominant. This reveals a new experimental dichotomy whereby functional sampling of Listeria-derived antigens in vivo but not in vitro is dependent on cross-presentation of exogenously derived antigen. Thus, under normal physiological circumstances, cross-presentation is demonstrated to play an essential role in priming CD8 T cell responses to bacteria

A supramolecular peptide nanofiber templated Pd nanocatalyst for efficient Suzuki coupling reactions under aqueous conditions

Cataloged from PDF version of article.A bioinspired peptide amphiphile nanofiber template for formation of one-dimensional Pd nanostructures is demonstrated. The Pd and peptide nanocatalyst system enabled efficient catalytic activity in Suzuki coupling reactions in water at room temperature. The nanocatalyst system can be easily separated and reused in successive reactions without significant loss in activity and structural integrity. This journal is © 2012 The Royal Society of Chemistry