Domains of invasion organelle proteins from apicomplexan parasites are homologous with the Apple domains of blood coagulation factor XI and plasma pre-kallikrein and are members of the PAN module superfamily

AbstractMicronemes are specialised organelles, found in all apicomplexan parasites, which secrete molecules that are essential for parasite attachment to and invasion of host cells. Regions of several microneme proteins have sequence similarity to the Apple domains (A-domains) of blood coagulation factor XI (FXI) and plasma pre-kallikrein (PK). We have used mass spectrometry on a recombinant-expressed, putative A-domain from the microneme protein EtMIC5 from Eimeria tenella, to demonstrate that three intramolecular disulphide bridges are formed. These bridges are analogous to those that stabilise A-domains in FXI and PK. The data confirm that the apicomplexan domains are structural homologues of A-domains and are therefore novel members of the PAN module superfamily, which also includes the N-terminal domains of members of the plasminogen/hepatocyte growth factor family. The role of A-domains/PAN modules in apicomplexan parasites is not known, but their presence in the microneme suggests that they may be important for mediating protein–protein or protein–carbohydrate interactions during parasite attachment and host cell invasion

Discovery of the heavily obscured supernova 2002cv

On the 13th of May 2002, supernova 2002cv was discovered using a near-infrared camera working at the AZT-24 1.1m telescope at Campo Imperatore (AQ-Italy). After the infrared detection a simultaneous photometric follow-up was started at optical wavelengths. The preliminary results confirm a heavily obscured object with a V-K color not lower than 6 magnitudes, making SN 2002cv the most reddened supernova ever observed. This finding, along with the recent discovery of another obscured supernova, suggests a critical revision of the rates known to date. The estimate of the visual extinction and the light curves are provided here. These latter indicate that our SN 2002cv observations are the earliest available for a type-Ia supernova at IR wavelengths.Comment: 4 page

The Population of Tiny Near-Earth Objects Observed by NEOWISE

Only a very small fraction of the asteroid population at size scales comparable to the object that exploded over Chelyabinsk, Russia has been discovered to date, and physical properties are poorly characterized. We present previously unreported detections of 106 close approaching near-Earth objects (NEOs) by the Wide-field Infrared Survey Explorer mission's NEOWISE project. These infrared observations constrain physical properties such as diameter and albedo for these objects, many of which are found to be smaller than 100 m. Because these objects are intrinsically faint, they were detected by WISE during very close approaches to the Earth, often at large apparent on-sky velocities. We observe a trend of increasing albedo with decreasing size, but as this sample of NEOs was discovered by visible light surveys, it is likely that selection biases against finding small, dark NEOs influence this finding.Comment: Accepted to Ap

A Geospatial Semantic Enrichment and Query Service for Geotagged Photographs

With the increasing abundance of technologies and smart devices, equipped with a multitude of sensors for sensing the environment around them, information creation and consumption has now become effortless. This, in particular, is the case for photographs with vast amounts being created and shared every day. For example, at the time of this writing, Instagram users upload 70 million photographs a day. Nevertheless, it still remains a challenge to discover the “right” information for the appropriate purpose. This paper describes an approach to create semantic geospatial metadata for photographs, which can facilitate photograph search and discovery. To achieve this we have developed and implemented a semantic geospatial data model by which a photograph can be enrich with geospatial metadata extracted from several geospatial data sources based on the raw low-level geo-metadata from a smartphone photograph. We present the details of our method and implementation for searching and querying the semantic geospatial metadata repository to enable a user or third party system to find the information they are looking for

A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence

Long RNAs often exist as multiple conformers in equilibrium. For the genomes of single-stranded RNA viruses, one of these conformers must include a compacted state allowing the RNA to be confined within the virion. We have used single molecule fluorescence correlation spectroscopy to monitor the conformations of viral genomes and sub-fragments in the absence and presence of coat proteins. Cognate RNA-coat protein interactions in two model viruses cause a rapid collapse in the hydrodynamic radii of their respective RNAs. This is caused by protein binding at multiple sites on the RNA that facilitate additional protein-protein contacts. The collapsed species recruit further coat proteins to complete capsid assembly with great efficiency and fidelity. The specificity in RNA-coat protein interactions seen at single-molecule concentrations reflects the packaging selectivity seen for such viruses in vivo. This contrasts with many in vitro reassembly measurements performed at much higher concentrations. RNA compaction by coat protein or polycation binding are distinct processes, implying that defined RNA-coat protein contacts are required for assembly

The Broad Absorption Line Tidal Disruption Event iPTF15af: Optical and Ultraviolet Evolution

We present multi-wavelength observations of the tidal disruption event (TDE) iPTF15af, discovered by the intermediate Palomar Transient Factory (iPTF) survey at redshift $z=0.07897$. The optical and ultraviolet (UV) light curves of the transient show a slow decay over five months, in agreement with previous optically discovered TDEs. It also has a comparable black-body peak luminosity of $L_{\rm{peak}} \approx 1.5 \times 10^{44}$ erg/s. The inferred temperature from the optical and UV data shows a value of (3$-$5) $\times 10^4$ K. The transient is not detected in X-rays up to $L_X < 3 \times 10^{42}$erg/s within the first five months after discovery. The optical spectra exhibit two distinct broad emission lines in the He II region, and at later times also H$\alpha$ emission. Additionally, emission from [N III] and [O III] is detected, likely produced by the Bowen fluorescence effect. UV spectra reveal broad emission and absorption lines associated with high-ionization states of N V, C IV, Si IV, and possibly P V. These features, analogous to those of broad absorption line quasars (BAL QSOs), require an absorber with column densities $N_{\rm{H}} > 10^{23}$ cm$^{-2}$. This optically thick gas would also explain the non-detection in soft X-rays. The profile of the absorption lines with the highest column density material at the largest velocity is opposite that of BAL QSOs. We suggest that radiation pressure generated by the TDE flare at early times could have provided the initial acceleration mechanism for this gas. Spectral UV line monitoring of future TDEs could test this proposal.Comment: 20 pages, 12 figures, published in Ap

Cosmology from Type Ia Supernovae

This presentation reports on first evidence for a low-mass-density/positive-cosmological-constant universe that will expand forever, based on observations of a set of 40 high-redshift supernovae. The experimental strategy, data sets, and analysis techniques are described. More extensive analyses of these results with some additional methods and data are presented in the more recent LBNL report #41801 (Perlmutter et al., 1998; accepted for publication in Ap.J.), astro-ph/9812133 . This Lawrence Berkeley National Laboratory reprint is a reduction of a poster presentation from the Cosmology Display Session #85 on 9 January 1998 at the American Astronomical Society meeting in Washington D.C. It is also available on the World Wide Web at http://supernova.LBL.gov/ This work has also been referenced in the literature by the pre-meeting abstract citation: Perlmutter et al., B.A.A.S., volume 29, page 1351 (1997).Comment: 9 pages, 8 color figs. Presented at Jan '98 AAS Meeting, also cited as BAAS,29,1351(1997). Archived here in response to requests; see more extensive analyses in ApJ paper (astro-ph/9812133

Discovery of a Supernova Explosion at Half the Age of the Universe and its Cosmological Implications

The ultimate fate of the universe, infinite expansion or a big crunch, can be determined by measuring the redshifts, apparent brightnesses, and intrinsic luminosities of very distant supernovae. Recent developments have provided tools that make such a program practicable: (1) Studies of relatively nearby Type Ia supernovae (SNe Ia) have shown that their intrinsic luminosities can be accurately determined; (2) New research techniques have made it possible to schedule the discovery and follow-up observations of distant supernovae, producing well over 50 very distant (z = 0.3 -- 0.7) SNe Ia to date. These distant supernovae provide a record of changes in the expansion rate over the past several billion years. By making precise measurements of supernovae at still greater distances, and thus extending this expansion history back far enough in time, we can distinguish the slowing caused by the gravitational attraction of the universe's mass density Omega_M from the effect of a possibly inflationary pressure caused by a cosmological constant Lambda. We report here the first such measurements, with our discovery of a Type Ia supernova (SN 1997ap) at z = 0.83. Measurements at the Keck II 10-m telescope make this the most distant spectroscopically confirmed supernova. Over two months of photometry of SN 1997ap with the Hubble Space Telescope and ground-based telescopes, when combined with previous measurements of nearer SNe Ia, suggests that we may live in a low mass-density universe. Further supernovae at comparable distances are currently scheduled for ground and space-based observations.Comment: 12 pages and 4 figures (figure 4 is repeated in color and black and white) Nature, scheduled for publication in the 1 January, 1998 issue. Also available at http://www-supernova.lbl.go