85 research outputs found
Regulation of the F0F1-ATP synthase: The conformation of subunit ε might be determined by directionality of subunit γ rotation
AbstractF0F1-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the γεc∼10-subunits complex relative to the rest of the enzyme.In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgADP. Subunit ε also modulates the activity: its conformation can change from a contracted to extended form with C-terminus stretched towards F1. The latter form ihnibits ATP hydrolysis (but not synthesis).We propose that the directionality of the coiled-coil subunit γ rotation determines whether subunit ε is in contracted or extended form. Block of rotation by MgADP presumably induces the extended conformation of subunit ε. This conformation might serve as a safety lock, stabilizing the ADP-inhibited state upon de-energization and preventing spontaneous re-activation and wasteful ATP hydrolysis. The hypothesis merges the known regulatory effects of ADP, protonmotive force and conformational changes of subunit ε into a consistent picture
The role of subunit epsilon in the catalysis and regulation of FOF1-ATP synthase
AbstractThe regulation of ATP synthase activity is complex and involves several distinct mechanisms. In bacteria and chloroplasts, subunit epsilon plays an important role in this regulation, (i) affecting the efficiency of coupling, (ii) influencing the catalytic pathway, and (iii) selectively inhibiting ATP hydrolysis activity. Several experimental studies indicate that the regulation is achieved through large conformational transitions of the α-helical C-terminal domain of subunit epsilon that occur in response to membrane energization, change in ATP/ADP ratio or addition of inhibitors. This review summarizes the experimental data obtained on different organisms that clarify some basic features as well as some molecular details of this regulatory mechanism. Multiple functions of subunit epsilon, its role in the difference between the catalytic pathways of ATP synthesis and hydrolysis and its influence on the inhibition of ATP hydrolysis by ADP are also discussed
The effect of mutations γM23K and βL249Q on ADP-inhibition of H+-FOF1-ATP-synthase in Escherichia coli
The cytochrome bc1 complex of Rhodobacter capsulatus: ubiquinol oxidation in a dimeric Q-cycle?
AbstractWe studied the cytochrome bc1 complex (hereafter bc) by flash excitation of Rhodobacter capsulatus chromatophores. The reduction of the high-potential heme bh of cytochrome b (at 561 nm) and of cytochromes c (at 552 nm) and the electrochromic absorption transients (at 524 nm) were monitored after the first and second flashes of light, respectively. We kept the ubiquinone pool oxidized in the dark and concerned for the ubiquinol formation in the photosynthetic reaction center only after the second flash. Surprisingly, the first flash caused the oxidation of about one ubiquinol per bc dimer. Based on these and other data we propose a dimeric Q-cycle where the energetically unfavorable oxidation of the first ubiquinol molecule by one of the bc monomers is driven by the energetically favorable oxidation of the second ubiquinol by the other bc monomer resulting in a pairwise oxidation of ubiquinol molecules by the dimeric bc in the dark. The residual unpaired ubiquinol supposedly remains on the enzyme and is then oxidized after the first flash
S1.17 Function of epsilon subunit in bacillus PS3 FOF1F0F1-ATP synthase: C-terminal helices of epsilon conduct the substrate specificity toward nucleoside triphosphates
An electromagnetic shashlik calorimeter with longitudinal segmentation
A novel technique for longitudinal segmentation of shashlik calorimeters has
been tested in the CERN West Area beam facility. A 25 tower very fine samplings
e.m. calorimeter has been built with vacuum photodiodes inserted in the first 8
radiation lengths to sample the initial development of the shower. Results
concerning energy resolution, impact point reconstruction and electron/pion
separation are reported.Comment: 13 pages, 12 figure
A shashlik calorimeter with longitudinal segmentation for a linear collider
Two techniques for longitudinal segmentation of shashlik calorimeters are proposed. Beam tests have demonstrated the technical feasibility of longitudinally segmented shashlik calorimeters in which longitudinal sampling is performed by lateral vacuum photodiodes or by using two scintillator types with different decay times. Results concerning energy resolution and e/π separation are presente
An electromagnetic shashlik calorimeter with longitudinal segmentation
A novel technique for longitudinal segmentation of shashlik calorimeters has been tested in the CERN West Area beam facility. A 25 tower e.m. calorimeter has been built with vacuum photodiodes inserted in the first 8 radiation lengths to sample the initial development of the shower. Results concerning energy resolution, impact point reconstruction and separation are reported.A novel technique for longitudinal segmentation of shashlik calorimeters has been tested in the CERN West Area beam facility. A 25 tower very fine samplings e.m. calorimeter has been built with vacuum photodiodes inserted in the first 8 radiation lengths to sample the initial development of the shower. Results concerning energy resolution, impact point reconstruction and electron/pion separation are reported
The native architecture of a photosynthetic membrane
In photosynthesis, the harvesting of solar energy and its subsequent conversion into a stable charge separation are dependent upon an interconnected macromolecular network of membrane-associated chlorophyll–protein complexes. Although the detailed structure of each complex has been determined, the size and organization of this network are unknown. Here we show the use of atomic force microscopy to directly reveal a native bacterial photosynthetic membrane. This first view of any multi-component membrane shows the relative positions and associations of the photosynthetic complexes and reveals crucial new features of the organization of the network: we found that the membrane is divided into specialized domains each with a different network organization and in which one type of complex predominates. Two types of organization were found for the peripheral light-harvesting LH2 complex. In the first, groups of 10–20 molecules of LH2 form light-capture domains that interconnect linear arrays of dimers of core reaction centre (RC)–light-harvesting 1 (RC–LH1–PufX) complexes; in the second they were found outside these arrays in larger clusters. The LH1 complex is ideally positioned to function as an energy collection hub, temporarily storing it before transfer to the RC where photochemistry occurs: the elegant economy of the photosynthetic membrane is demonstrated by the close packing of these linear arrays, which are often only separated by narrow 'energy conduits' of LH2 just two or three complexes wide
Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation.
ATP synthase is a membrane-bound rotary motor enzyme that is critical for cellular energy metabolism in all kingdoms of life. Despite conservation of its basic structure and function, autoinhibition by one of its rotary stalk subunits occurs in bacteria and chloroplasts but not in mitochondria. The crystal structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli described here reveals the structural basis for this inhibition. The C-terminal domain of subunit ɛ adopts a heretofore unknown, highly extended conformation that inserts deeply into the central cavity of the enzyme and engages both rotor and stator subunits in extensive contacts that are incompatible with functional rotation. As a result, the three catalytic subunits are stabilized in a set of conformations and rotational positions distinct from previous F(1) structures
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