Hand-held lactate analyzer as a tool for the real-time measurement of physical fatigue before slaughter and pork quality prediction

The objectives of this study were to assess the relationship between blood lactate variation measured at the plant, and pork quality variation on a large sample size and under commercial preslaughter handling conditions. A total of 600 pigs were randomly chosen on arrival at a commercial slaughter plant and blood samples taken from the ear vein at unloading (UN), after lairage (LA), in the restrainer (RE; before stunning) and at exsanguination (EX) were analysed for lactate content using a Lactate Scout Analyzer (LSA). In order to have a large range of measures, pigs were distributed into two groups; one kept in lairage overnight (G1) and the other for 2 to 3 h (G2) before slaughter. Meat quality was assessed in the Longissimus thoracis (LT), Semimembranosus (SM) and Adductor (AD) muscles by measuring the pH 30 min postmortem (pH1) and at 24 h postmortem (pHu), the colour and the drip loss. Blood lactate levels did not differ between G1 and G2 (P>0.05). A reduced muscle lactate and glucose contents (P=0.02 and P=0.004, respectively) resulting in a lower (P<0.001) glycolytic potential (GP) was observed in the LT muscle of G1 pigs when compared with G2 loins. In the LT muscle of G1 pigs, the lower GP resulted in an increased pHu (r= 120.67; P<0.001), decreased drip loss (r=0.57; P<0.001) and darker colour (r=0.50; P<0.001) compared with G2. In both G1 and G2 pigs, the lower GP was correlated to higher pHu value in the SM and AD muscles (r= 120.73; P<0.001). The greatest correlation was observed in G2 between blood lactate levels at LA and pHu value of the SM and AD muscles (r=0.46 and r=0.44, respectively; P<0.001 for both muscles). The second greatest correlation was found between blood lactate levels at EX and pH1 value in the SM muscle in both groups (r= 120.37 and r= 120.41, respectively; P<0.001 for both groups). Based on the results of this study, it appears that blood lactate levels, as measured by the LSA, reliably reflect the physiological response of pigs to perimortem stress and may help explain the variation in pork quality

Meat quality of pigs submitted to diferent water spray periods.

Projeto/Plano de Ação: 02.06.01.001

Stress levels of pigs submitted to three water spray periods.

Projeto/Plano de Ação: 02.06.01.001

Comportamento dos suínos submetidos a diferentes períodos de aspersão e descanso.

Projeto/Plano de Ação: 02.06.01.001

Uso de aspersão em suínos nas baias de espera: implicações no bem-estar.

Projeto/Plano de Ação: 03.08.60800-01

Efeito na qualidade da carne de suínos submetidos a diferentes períodos de aspersão.

Projeto/Plano de Ação: 03.08.60800-01

Hand-held lactate analyzer as a tool for the real-time measurement of blood lactate during slaughter and pork quality prediction

A total of 600 pigs was randomly chosen on arrival at a commercial slaughter plant and sampled for lactate analysis from the ear vein using a Lactate Scout Analyzer (LSA) at unloading (UN), after lairage (LA), in the restrainer (RE; before stunning), and from the ear vein (EX1) and the bleeding incision (EX2) at exsanguination. Pigs were distributedinto two pen groups, one kept in lairage overnight (G1) and the other kept between 2 and 3 h before slaughter (G2).Meat quality was assessed in the Longissimus dorsi(LD), Semimembranosus (SM) and Adductor(AD) muscles. Data were analyzed using Spearman correlations and the MIXED procedure of SAS. Greater (P=0.009) levels of blood lactate were found in pigs laired longer, which resulted in LD and SM muscles with greater pHu (P=0.03 and P=0.001, respectively), as well as lower L* (P=0.005and P=0.008, respectively)and drip loss (P=0.01 and P=0.02, respectively). The greatest correlation with lactate levels was observed at LA with pHu value of the SM and AD muscles (r=0.40; P<0.001). LSA lactate levels reliably reflect the physiological response of pigs to preslaughter procedures and may help explain the variation in pork quality as measured in the ham muscles

Determinação do ritmo ultradiano do cortisol na saliva de fêmeas suínas em peso de abate.

bitstream/item/58070/1/CUsersPiazzonDocuments460.pdfProjeto n. 02.06.10.100-02