IMPACT OF SOME BIO-STIMULANTS ON PERFORMANCE OF ZINNIA ELEGANS SEEDLINGS

The purpose of this study was to investigate the impacts of Spirulina platensis algae extract (seaweed) and Saccharomyces cerevisiae yeast extract as bio-stimulants on the growth, flowering, and chemical composition of the Zinnia elegans plants during 2021 and 2022 seasons. When the seedlings reached about 10 cm in length, they were transplanted into individual pots filled with a mixture of clay and sand (1:1, v/v). Seedlings were sprayed with algae extract at 0.5 and 1% and yeast extract at 3 and 6 g/l however, control plants were sprayed with distilled water. The obtained results indicated that algae extract at 1% treatment increased all vegetative parameters including plant height, stem diameter, number of branches/plant, number of leaves/plant, root length, leaf area, shoot and root fresh and dry weights, flowering parameters including number of inflorescences/plant, inflorescence F.W. and D.W. in both seasons and inflorescence diameter in the second season only. The chemical composition was also positively affected by the same treatment and gave the highest values for photosynthetic pigments, total amino acids, crude protein, macronutrient elements (N, P and K%), total sugar content and total indoles followed by plants sprayed with yeast extract at 6 g/l for all the mentioned parameters during both seasons. It was concluded that algae extract at 1% or yeast extract at 6 g/l can be used as bio-stimulants to boost the growth of the Zinnia elegans plan

SYNTHESIS AND EVALUATION OF ETHYL (4-(N-(THIAZOL-2-YL) SULFAMOYL) PHENYL)CARBAMATE (TSPC) AS A CORROSION INHIBITOR FOR MILD STEEL IN 0.1M HCL

Laboratory synthesized ethyl (4-(N-(thiazol-2-yl)sulfamoyl)phenyl)carbamate (TSPC), characterized by 1H NMR spectroscopy, was evaluated as corrosion inhibitor of mild steel in 0.1M HCl using electrochemical techniques. Open circuit potential, potentiodynamic polarization and impedance spectroscopy were used to evaluate the inhibition efficiency of (TSPC) at various concentrations. The obtained electrochemical data indicated that (TSPC) acts as moderate corrosion inhibitor for mild steel in acidic media. It is found that the inhibition efficiency increases with the concentration of the inhibitor till 400ppm. The adsorption isotherm involving physisorption of (TSPC) at room temperature and the experimental data complied to the Langmuir adsorption isotherms and the negative values of the Gibb’s free energy of adsorption obtained suggested that inhibitor molecules have been spontaneously adsorbed onto the mild steel surface

2-Methyl-6-(4-aminophenyl)-4,5-dihydro-3(2H)-pyridazinone Synthon for Some New Annelated 1,2,3-Selena/Thiadiazoles and 2H-Diazaphospholes with Anticipated Biological Activity and Quantum Chemical Calculations

A convenient and efficient synthetic protocol for the new selenadiazole. Thiadiazole and diazaphosphole derivatives incorporating a pyridazine moiety originating from 4-(4-aminophenyl)-4-oxobutanoic acid (1) were described. All newly synthesized compounds were evaluated for their antimicrobial activity using the disk diffusion method, and their cytotoxicity was evaluated against brine shrimp lethality bioassay. Using density functional theory (DFT), the frontier molecular orbital (FMO) and molecular electrostatic potential (MEPS) were studied to estimate the chemical reactivity and kinetic stability of each structure. Therefore, global descriptor parameters like electronegativity (chi), chemical hardness (eta), and global softness (sigma) were calculated. Consequently, the attained results were compared with the experimental data of the biological activity of the studied structures

Synthesis of some new 4-(2,4-dimethyl-phenyl)-2H-phthalazinone derivatives

Aroylation of m-xylene by phthalic anhydride under Friedel craft’s reaction conditions to afford the corresponding o-aroylbenzoic acid derivative (2), which followed by cyclization reaction with hydroxylamine hydrochloride to produce the correlating benzoxazinone derivative (3), which was utilized as a precursor for the formation of some novel phthalazinone derivatives (4˗6). This transformation was achieved by interactions with thiosemicarbazide, thiocarbohydrazide, and hydrazine monohydrate and/or ammonium acetate under suitable conditions. 4-Substituted-1(2H)- phthalazinone derivative (6), which undergoes N-alkylation using ethyl bromoacetate to produce the phthalazinone acetic acid ethyl ester derivative (7), that followed by the interaction with hydrazine monohydrate to afford the phthalazinone acetic acid hydrazide derivative (8). The latter product was conducted with different aromatic acid compounds in presence of POCl3, to give the correlating oxadiazoles (9,10). The chemical structures of all the synthesized compounds are confirmed using physical and spectral data analyses like FT- IR, 1H-NMR, and mass spectroscopy

Sequence Heterogeneity in NS5A of Hepatitis C Virus Genotypes 2a and 2b and Clinical Outcome of Pegylated-Interferon/Ribavirin Therapy

Pegylated-interferon plus ribavirin (PEG-IFN/RBV) therapy is a current standard treatment for chronic hepatitis C. We previously reported that the viral sequence heterogeneity of part of NS5A, referred to as the IFN/RBV resistance-determining region (IRRDR), and a mutation at position 70 of the core protein of hepatitis C virus genotype 1b (HCV-1b) are significantly correlated with the outcome of PEG-IFN/RBV treatment. Here, we aimed to investigate the impact of viral genetic variations within the NS5A and core regions of other genotypes, HCV-2a and HCV-2b, on PEG-IFN/RBV treatment outcome. Pretreatment sequences of NS5A and core regions were analyzed in 112 patients infected with HCV-2a or HCV-2b, who were treated with PEG-IFN/RBV for 24 weeks and followed up for another 24 weeks. The results demonstrated that HCV-2a isolates with 4 or more mutations in IRRDR (IRRDR[2a]≥4) was significantly associated with rapid virological response at week 4 (RVR) and sustained virological response (SVR). Also, another region of NS5A that corresponds to part of the IFN sensitivity-determining region (ISDR) plus its carboxy-flanking region, which we referred to as ISDR/+C[2a], was significantly associated with SVR in patients infected with HCV-2a. Multivariate analysis revealed that IRRDR[2a]≥4 was the only independent predictive factor for SVR. As for HCV-2b infection, an N-terminal half of IRRDR having two or more mutations (IRRDR[2b]/N≥2) was significantly associated with RVR, but not with SVR. No significant correlation was observed between core protein polymorphism and PEG-IFN/RBV treatment outcome in HCV-2a or HCV-2b infection. Conclusion: The present results suggest that sequence heterogeneity of NS5A of HCV-2a (IRRDR[2a]≥4 and ISDR/+C[2a]), and that of HCV-2b (IRRDR[2b]/N≥2) to a lesser extent, is involved in determining the viral sensitivity to PEG-IFN/RBV therapy

Green synthesized extracts/Au complex of <i>Phyllospongia lamellosa</i>:unrevealing the anti-cancer and anti-bacterial potentialities, supported by metabolomics and molecular modeling

The anti-cancer and anti-bacterial potential of the Red Sea sponge Phyllospongia lamellosa in its bulk (crude extracts) and gold nanostructure (loaded on gold nanaoparticles) were investigated. Metabolomics analysis was conducted, and subsequently, molecular modeling studies were conducted to explore and anticipate the P. lamellosa secondary metabolites and their potential target for their various bioactivities. The chloroformic extract (CE) and ethyl acetate extract (EE) of the P. lamellosa predicted to include bioactive lipophilic and moderately polar metabolites, respectively, were used to synthesize gold nanoparticles (AuNPs). The prepared AuNPs were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and UV–vis spectrophotometric analyses. The cytotoxic activities were tested against MCF-7, MDB-231, and MCF-10A. Moreover, the anti-bacterial, antifungal, and anti-biofilm activity were assessed. Definite classes of metabolites were identified in CE (terpenoids) and EE (brominated phenyl ethers and sulfated fatty amides). Molecular modeling involving docking and molecular dynamics identified Protein-tyrosine phosphatase 1B (PTP1B) as a potential target for the anti-cancer activities of terpenoids. Moreover, CE exhibited the most powerful activity against breast cancer cell lines, matching our molecular modeling study. On the other hand, only EE was demonstrated to possess powerful anti-bacterial and anti-biofilm activity against Escherichia coli. In conclusion, depending on their bioactive metabolites, P. lamellosa-derived extracts, after being loaded on AuNPs, could be considered anti-cancer, anti-bacterial, and anti-biofilm bioactive products. Future work should be completed to produce drug leads

The impact of laparoscopic ovarian drilling on AMH and ovarian reserve: a meta-analysis

Laparoscopic ovarian drilling (LOD) has been widely used as an effective treatment of anovulatory women with polycystic ovarian syndrome (PCOS). However, there has been a growing concern over a possible damaging effect of this procedure on ovarian reserve. The objective of this study was to investigate the hypothesis that LOD compromises ovarian reserve as measured by post-operative changes in circulating anti-Müllerian hormone (AMH). This meta-analysis included all cohort studies as well as randomised controlled trials (RCTs) investigating serum AMH concentrations and other ovarian reserve markers in women with PCOS undergoing LOD. Various databases were searched including MEDLINE, EMBASE, Dynamed Plus, ScienceDirect, TRIP database, ClinicalTrials.gov and Cochrane Library from January 2000 to December 2016. Sixty studies were identified, of which seven were deemed eligible for this review. AMH data were extracted from each study and entered into the RevMan software to calculate the weighted mean difference (WMD) between pre- and post-operative values. Pooled analysis of all studies (n = 442) revealed a statistically significant decline in serum AMH concentration after LOD (WMD −2.13 ng/mL; 95% confidence interval (CI) −2.97 to −1.30). Subgroup analysis based on duration of follow-up, AMH kit, laterality of surgery and amount of energy applied during LOD consistently showed a statistically significant fall in serum AMH concentration. In conclusion, although LOD seems to markedly reduce circulating AMH, it remains uncertain whether this reflects a real damage to ovarian reserve or normalisation of the high pre-operative serum AMH levels. Further long-term studies on ovarian reserve after LOD are required to address this uncertainty

New Longitudinal Waves in Electron-Positron-Ion Quantum Plasmas

A general quantum dispersion equation for electron-positron(hole)-ion quantum plasmas is derived and studied for some interesting cases. In an electron-positron degenerate Fermi gas, with or without the Madelung term, a new type of zero sound waves are found. Whereas in an electron-hole plasmas a new longitudinal quantum waves are revealed, which have no analogies in quantum electron-ion plasmas. The excitation of these quantum waves by a low-density monoenergetic straight electron beam is examined. Furthermore, the KdV equation for novel quantum waves is derived and the contribution of the Madelung term in the formation of the KdV solitons is discussed