Mutations in the Arabidopsis Peroxisomal ABC Transporter COMATOSE Allow Differentiation between Multiple Functions In Planta: Insights from an Allelic Series

COMATOSE (CTS), the Arabidopsis homologue of human Adrenoleukodystrophy protein (ALDP), is required for import of substrates for peroxisomal β-oxidation. A new allelic series and a homology model based on the bacterial ABC transporter, Sav1866, provide novel insights into structure-function relations of ABC subfamily D proteins. In contrast to ALDP, where the majority of mutations result in protein absence from the peroxisomal membrane, all CTS mutants produced stable protein. Mutation of conserved residues in the Walker A and B motifs in CTS nucleotide-binding domain (NBD) 1 resulted in a null phenotype but had little effect in NBD2, indicating that the NBDs are functionally distinct in vivo. Two alleles containing mutations in NBD1 outside the Walker motifs (E617K and C631Y) exhibited resistance to auxin precursors 2,4-dichlorophenoxybutyric acid (2,4-DB) and indole butyric acid (IBA) but were wild type in all other tests. The homology model predicted that the transmission interfaces are domain-swapped in CTS, and the differential effects of mutations in the conserved "EAA motif" of coupling helix 2 supported this prediction, consistent with distinct roles for each NBD. Our findings demonstrate that CTS functions can be separated by mutagenesis and the structural model provides a framework for interpretation of phenotypic data

Suppression of combustion oscillations with mechanical devices Interim report

Static rocket thrust chamber simulator for cylindrical cold flow-type apparatus desig

Mixing effectiveness in the Apollo oxygen tanks of spin-up and rotation-reversal maneuvers

Two-dimensional simulations of stratified flows in the Apollo oxygen tanks have been used to estimate the mixing effectiveness of spin-up and rotation-reversal maneuvers. Calculations have been made for square and circular cylindrical tank geometries. Differences arising from heater position on the tank wall or near the center of the tank have been investigated. In the event of a prolonged period without normal maneuvers, the potential pressure decay (drop in pressure that would result from adiabatic mixing) can be suppressed by more than a factor of two through the use of spin-up and rotation-reversal maneuvers. Changes in rotation rate of order three revolutions per hour or greater are sufficient for this purpose

Sub-surface damage issues for effective fabrication of large optics

A new ultra precision large optics grinding machine, BoX®has been developed at Cranfield University. BoX®islocated at the UK's Ultra Precision Surfaces laboratory at the OpTIC Technium. This machine offers a rapidand economic solution for grinding large off-axis aspherical and free-form optical components.This paper presents an analysis of subsurface damage assessments of optical ground materials produced usingdiamond resin bonded grinding wheels. The specific materials used, Zerodur®and ULE®are currently understudy for making extremely large telescope (ELT) segmented mirrors such as in the E-ELT project.The grinding experiments have been conducted on the BoX®grinding machine using wheels with grits sizes of76 μm, 46 μm and 25 μm. Grinding process data was collected using a Kistler dynamometer platform. Thehighest material removal rate (187.5 mm3/s) used ensures that a 1 metre diameter optic can be ground in lessthan 10 hours. The surface roughness and surface profile were measured using a Form Talysurf. The subsurfacedamage was revealed using a sub aperture polishing process in combination with an etching technique.These results are compared with the targeted form accuracy of 1 μm p-v over a 1 metre part, surface roughnessof 50-150 nm RMS and subsurface damage in the range of 2-5 μm. This process stage was validated on a 400mm ULE®blank and a 1 metre hexagonal Z

The Nature of the H2-Emitting Gas in the Crab Nebula

Understanding how molecules and dust might have formed within a rapidly expanding young supernova remnant is important because of the obvious application to vigorous supernova activity at very high redshift. In previous papers, we found that the H2 emission is often quite strong, correlates with optical low-ionization emission lines, and has a surprisingly high excitation temperature. Here we study Knot 51, a representative, bright example, for which we have available long slit optical and NIR spectra covering emission lines from ionized, neutral, and molecular gas, as well as HST visible and SOAR Telescope NIR narrow-band images. We present a series of CLOUDY simulations to probe the excitation mechanisms, formation processes and dust content in environments that can produce the observed H2 emission. We do not try for an exact match between model and observations given Knot 51's ambiguous geometry. Rather, we aim to explain how the bright H2 emission lines can be formed from within the volume of Knot 51 that also produces the observed optical emission from ionized and neutral gas. Our models that are powered only by the Crab's synchrotron radiation are ruled out because they cannot reproduce the strong, thermal H2 emission. The simulations that come closest to fitting the observations have the core of Knot 51 almost entirely atomic with the H2 emission coming from just a trace molecular component, and in which there is extra heating. In this unusual environment, H2 forms primarily by associative detachment rather than grain catalysis. In this picture, the 55 H2-emitting cores that we have previously catalogued in the Crab have a total mass of about 0.1 M_sun, which is about 5% of the total mass of the system of filaments. We also explore the effect of varying the dust abundance. We discuss possible future observations that could further elucidate the nature of these H2 knots.Comment: 51 pages, 15 figures, accepted for publication in MNRAS, revised Figure 12 results unchange

Use of Nack Oriented Reliable Multicast (NORM) Protocol for Transport of Spacecraft Telemetry in Ground Networks

Frequently it is necessary to distribute spacecraft telemetry to multiple destinations on Internet Protocol (IP) networks. There are various methods of delivering this data, but when the data is high rate and there are many destinations IP multicast provides the best solution from a network perspective. However, IP multicast only supports the User Datagram Protocol (UDP) which is connectionless and unreliable. This nature of UDP and by extension the low priority treatment of UDP packets by some network hardware creates a difficult environment for distribution of high rate telemetry to a number of recipients over IP networks. NACK (Negative ACKnowledgment) Oriented Reliable Multicast (NORM) is defined by IETF (Internet Engineering Task Force) RFC (Request for Comments) 5740 in 2009 by B. Adamson et al. NORM provides a mechanism of retransmission and or forward error correction for UDP packets, potentially overcoming the limits of UDP for the transmission of spacecraft telemetry. Marshall Space Flight Centers (MSFC) Huntsville Operations Support Center (HOSC) manages the delivery of the International Space Station (ISS) payload science and health data. The ISS IP Ground Routed (IIGoR) network provides the delivery of the data from the ground stations to the control centers. HOSC personnel have been experimenting with the use of NORM on the link between the ground stations and the front end processor equipment in Huntsville, AL