Water Stress Alters Lignin Content And Related Gene Expression In Two Sugarcane Genotypes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The lignin deposition in the stem of two sugarcane genotypes was assessed on exposure to water stress. The lignin content and the morphoanatomical characterization of the stem indicated that IACSP94-2094 plants are more lignified than those of IACSP95-5000 genotype, under normal water supply conditions, which was especially associated with higher lignin contents in the rind of mature internodes. Water deficit had negative impact on the biomass production, mostly with IACSP94-2094 plants, possibly due to stress severity or higher susceptibility of that genotype during the stem-lengthening phase. Water deficit led to significant alterations in the expression levels of lignin biosynthesis genes and led to an approximate 60% increase of lignin content in the rind of young internodes in both genotypes. It is concluded that the young rind region was more directly affected by water stress and, depending on the genotype, a higher lignin accumulation may occur in the stem, thus implying lower quality biomass for bioethanol production.631947084720Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2008/58035-6

Lignin biosynthesis in sugarcane is affected by low temperature

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sugarcane is an abundant and promising source of plant biomass for biofuel production. The use of biomass for conversion to ethanol is limited by the recalcitrance of lignocellulosic material mainly due to the presence of lignin. This study characterised some aspects of lignification in the stem of two sugarcane genotypes grown under low (Cl') and warmer (HT - reference) temperatures. Stems were separated in young and mature culms and the culms were separated in cortex (rind) and medulla (pith). Plants of the genotype IACSP04-627, which has more lignin in the stem, grew better (fresh weight of the stem) than IACSP04-065 under HT. Cold negatively affected plant growth but apparently IACSPO4-065 was more sensitive than the other genotype. Lignin content was significantly increased in the young rind of IACSP04-627 plants at CT, what could not be directly correlated with the expression profile of genes of the monolignols biosynthesis. Lignin content in mature rind was reduced in IACSP04-065 plants exposed to CT, what could be correlated with the low expression level of the genes ShCAD2, ShCOMT1 and ShCCoAOMT1. The high expression of ShF5H occurred preferentially in mature pith of both sugarcane genotypes, what is possibly related with the early formation of the secondary cell wall induced by low temperature. In conclusion, lignin deposition in sugarcane under low temperature seems to be differentially regulated in rind and pith tissues and it is genotype-dependent. (C) 2015 Elsevier B.V. All rights reserved.Sugarcane is an abundant and promising source of plant biomass for biofuel production. The use of biomass for conversion to ethanol is limited by the recalcitrance of lignocellulosic material mainly due to the presence of lignin. This study characterised1203142FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORCNQP - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2008/58035-6]2008/58035-6SEM INFORMAÇÃOSEM INFORMAÇÃOThis study was funded by Fundac ̧ão de Amparo à Pesquisa doEstado de São Paulo (FAPESP – grant 2008/58035-6). The authors thank Coordenac ̧ão de Aperfeic ̧oamento de Pessoal de Nível Supe-rior (Capes – Brazil) and Conselho Nacional de DesenvolvimentoCien

Water Stress Alters Lignin Content and Related Gene Expression in Two Sugarcane Genotypes

The lignin deposition in the stem of two sugarcane genotypes was assessed on exposure to water stress. The lignin content and the morphoanatomical characterization of the stem indicated that IACSP94-2094 plants are more lignified than those of IACSP95-5000 genotype, under normal water supply conditions, which was especially associated with higher lignin contents in the rind of mature internodes. Water deficit had negative impact on the biomass production, mostly with IACSP94-2094 plants, possibly due to stress severity or higher susceptibility of that genotype during the stem-lengthening phase. Water deficit led to significant alterations in the expression levels of lignin biosynthesis genes and led to an approximate 60% increase of lignin content in the rind of young internodes in both genotypes. It is concluded that the young rind region was more directly affected by water stress and, depending on the genotype, a higher lignin accumulation may occur in the stem, thus implying lower quality biomass for bioethanol production