(Sp)-1-Diphenylphosphanyl-2-{(S)-[2-(diphenylphosphanyl)phenyl]hydroxymethyl}ferrocene

The absolute configuration of the title compound, [Fe(C5H5)(C36H29OP2)], is Sp at the ferrocene group and S at the asymmetric C atom. Both P atoms have a trigonal-pyramidal conformation. There is a short intramolecular C-H...P contact with an H...P distance of 2.56 Å. The hydroxy group is involved in an intramolecular O-H...[pi]phenyl interaction. The crystal packing shows five very weak intermolecular C-H...[pi] contacts, with H...Cg distances between 3.26 and 3.39 Å (Cg is the centroid of a phenyl or cyclopentadienyl ring). Key indicators: single-crystal X-ray study; T = 162 K; mean σ(C–C) = 0.004 Å; R factor = 0.038; wR factor = 0.083; data-to-parameter ratio = 22.3

(R p)-1-{(R)-(Dimethyl­amino)[2-(diphenyl­phosphan­yl)phen­yl]methyl}-2-(diphenyl­phosphan­yl)ferrocene chloro­form solvate

The absolute configuration of the title mol­ecule, [Fe(C5H5)(C38H34NP2)]·CHCl3, is R,R p. The mol­ecular structure is similar to the structure of the solvent-free compound [Fukuzawa, Yamamoto & Kikuchi (2007 ▶). J. Org. Chem. 72, 1514–1517], but some torsion angles about the P—Cphen­yl bonds differ by up to 25°. The P atoms and the N atom have a distorted trigonal-pyramidal geometry. The chloro­form solvate group donates a C—H⋯π bond to the central benzene ring and is also involved in six inter­molecular C—H⋯Cl contacts with H⋯Cl distances between 2.96 and 3.13 Å

Dichlorido(3-phenyl­indenyl­idene)bis­(triphenyl­phosphane)ruthenium(II) tetra­hydro­furan disolvate

The RuII atom in the title compound, [RuCl2(C15H10)(C18H15P)2]·2C4H8O, has a distorted square-pyramidal conformation. The P and Cl atoms are at the base of the pyramid and the Ru—Cindenyl­idene bond is in the axial position. The two Cl ligands and the two phosphane ligands are in trans positions. The Cl—Ru—Cl and P—Ru—P angles are 157.71 (2) and 166.83 (2)°, respectively. The two independent tetra­hydro­furan (THF) solvent mol­ecules are disordered. One THF mol­ecule was refined using a split-atom model. The second THF mol­ecule was accounted for by using program PLATON/SQUEEZE [Spek (2009 ▶). Acta Cryst. D65, 148–155]. The molecular conformation shows three intramolecular C—H⋯Cl contacts and two C—H⋯π interactions while the crystal packing features an intermolecular C—H⋯Cl contact and two very weak intermolecular C—H⋯π contacts

Enhanced functional detection of synaptic calcium-permeable AMPA receptors using intracellular NASPM

Calcium-permeable AMPA-type glutamate receptors (CP-AMPARs) contribute to many forms of synaptic plasticity and pathology. They can be distinguished from GluA2-containing calcium-impermeable AMPARs by the inward rectification of their currents, which reflects voltage-dependent channel block by intracellular spermine. However, the efficacy of this weakly permeant blocker is differentially altered by the presence of AMPAR auxiliary subunits - including transmembrane AMPAR regulatory proteins, cornichons and GSG1L - which are widely expressed in neurons and glia. This complicates the interpretation of rectification as a measure of CP-AMPAR expression. Here we show that inclusion of the spider toxin analogue 1‑naphthylacetyl spermine (NASPM) in the intracellular solution results in complete block of GluA1-mediated outward currents irrespective of the type of associated auxiliary subunit. In neurons from GluA2-knockout mice expressing only CP-AMPARs, intracellular NASPM, unlike spermine, completely blocks outward synaptic currents. Thus, our results identify a functional measure of CP-AMPARs, that is unaffected by their auxiliary subunit content

Treatment of Multisystem Inflammatory Syndrome in Children.

BACKGROUND: Evidence is urgently needed to support treatment decisions for children with multisystem inflammatory syndrome (MIS-C) associated with severe acute respiratory syndrome coronavirus 2. METHODS: We performed an international observational cohort study of clinical and outcome data regarding suspected MIS-C that had been uploaded by physicians onto a Web-based database. We used inverse-probability weighting and generalized linear models to evaluate intravenous immune globulin (IVIG) as a reference, as compared with IVIG plus glucocorticoids and glucocorticoids alone. There were two primary outcomes: the first was a composite of inotropic support or mechanical ventilation by day 2 or later or death; the second was a reduction in disease severity on an ordinal scale by day 2. Secondary outcomes included treatment escalation and the time until a reduction in organ failure and inflammation. RESULTS: Data were available regarding the course of treatment for 614 children from 32 countries from June 2020 through February 2021; 490 met the World Health Organization criteria for MIS-C. Of the 614 children with suspected MIS-C, 246 received primary treatment with IVIG alone, 208 with IVIG plus glucocorticoids, and 99 with glucocorticoids alone; 22 children received other treatment combinations, including biologic agents, and 39 received no immunomodulatory therapy. Receipt of inotropic or ventilatory support or death occurred in 56 patients who received IVIG plus glucocorticoids (adjusted odds ratio for the comparison with IVIG alone, 0.77; 95% confidence interval [CI], 0.33 to 1.82) and in 17 patients who received glucocorticoids alone (adjusted odds ratio, 0.54; 95% CI, 0.22 to 1.33). The adjusted odds ratios for a reduction in disease severity were similar in the two groups, as compared with IVIG alone (0.90 for IVIG plus glucocorticoids and 0.93 for glucocorticoids alone). The time until a reduction in disease severity was similar in the three groups. CONCLUSIONS: We found no evidence that recovery from MIS-C differed after primary treatment with IVIG alone, IVIG plus glucocorticoids, or glucocorticoids alone, although significant differences may emerge as more data accrue. (Funded by the European Union's Horizon 2020 Program and others; BATS ISRCTN number, ISRCTN69546370.)

Data Management Plan Implementation, Assessments, and Evaluations: Implications and Recommendations

Data management plans (DMPs) have become nearly a worldwide requirement for research funding. To meet these new funding agency expectations, information professionals across domains and the world have worked to create resources and services to successfully implement and sometimes assess DMPs. This essay presents a series of case studies from different institutions across the globe to highlight current practices and share recommendations for future work. A summary of various projects related to DMP implementation, assessment, and evaluation in different contexts provides a useful overview of current practices. The essay concludes with recommendations for practical oversight and scoring to improve DMPs’ utility in enabling the sharing of data