Cyclophilin-A is bound to through its peptidylprolyl isomerase domain to the cytoplasmic dynein motor protein complex

Although cyclophilin A (CyP-A) is a relatively abundant small immunophilin present in the cytoplasm of all mammalian cells, its general function(s) in the absence of the immunosuppressant drug cyclosporin A is not known. In contrast, the high molecular weight hsp90-binding immunophilins appear to play a role in protein trafficking in that they have been shown to link glucocorticoid receptor-hsp90 and p53.hsp90 complexes to the dynein motor protein for retrograde movement along microtubules. These immunophilins link to cytoplasmic dynein indirectly through the association of the immunophilin peptidylprolyl isomerase (PPIase) domain with dynamitin, a component of the dynein-associated dynactin complex (Galigniana, M. D., Harrell, J. M., O'Hagen, H. M., Ljungman, M., and Pratt, W. B. (2004) J. Biol. Chem. 279, 22483-22489). Here, we show that CyP-A exists in native heterocomplexes containing cytoplasmic dynein that can be formed in cell-free systems. Prolyl isomerase activity is not required for forming the dynein complex, but the PPIase domain fragment of FKBP52 blocks complex formation and CyP-A binds to dynamitin in a PPIase domain-dependent manner. CyP-A heterocomplexes containing tubulin and dynein can be formed in cytosol prepared under microtubule-stabilizing conditions, and CyP-A colocalizes in mouse fibroblasts with microtubules. Colocalization with microtubules is disrupted by overexpression of the PPIase domain fragment. Thus, we conclude that CyP-A associates in vitro and in vivo with the dynein/dynactin motor protein complex and we suggest that CyP-A may perform a general function related to the binding of cargo for retrograde movement along microtubules.Fil: Galigniana, Mario Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Michigan; Estados UnidosFil: Morishima, Yoshihiro. University of Michigan; Estados UnidosFil: Gallay, Philippe A.. The Scripps Research Institute; Estados UnidosFil: Pratt, William B.. University of Michigan; Estados Unido

Equational reasoning with context-free families of string diagrams

String diagrams provide an intuitive language for expressing networks of interacting processes graphically. A discrete representation of string diagrams, called string graphs, allows for mechanised equational reasoning by double-pushout rewriting. However, one often wishes to express not just single equations, but entire families of equations between diagrams of arbitrary size. To do this we define a class of context-free grammars, called B-ESG grammars, that are suitable for defining entire families of string graphs, and crucially, of string graph rewrite rules. We show that the language-membership and match-enumeration problems are decidable for these grammars, and hence that there is an algorithm for rewriting string graphs according to B-ESG rewrite patterns. We also show that it is possible to reason at the level of grammars by providing a simple method for transforming a grammar by string graph rewriting, and showing admissibility of the induced B-ESG rewrite pattern.Comment: International Conference on Graph Transformation, ICGT 2015. The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-319-21145-9_

Constraining the initial temperature and shear viscosity in a hybrid hydrodynamic model of sNN\sqrt{s_{NN}}=200 GeV Au+Au collisions using pion spectra, elliptic flow, and femtoscopic radii

A new framework for evaluating hydrodynamic models of relativistic heavy ion collisions has been developed. This framework, a Comprehesive Heavy Ion Model Evaluation and Reporting Algorithm (CHIMERA) has been implemented by augmenting UVH 2+1D viscous hydrodynamic model with eccentricity fluctuations, pre-equilibrium flow, and the Ultra-relativistic Quantum Molecular Dynamic (UrQMD) hadronic cascade. A range of initial temperatures and shear viscosity to entropy ratios were evaluated for four initial profiles, $N_{part}$ and $N_{coll}$ scaling with and without pre-equilibrium flow. The model results were compared to pion spectra, elliptic flow, and femtoscopic radii from 200 GeV Au+Au collisions for the 0--20% centrality range.Two sets of initial density profiles, $N_{part}$ scaling with pre-equilibrium flow and $N_{coll}$ scaling without were shown to provide a consistent description of all three measurements.Comment: 21 pages, 32 figures, version 3 includes additional text for clarification, division of figures into more manageable units, and placement of chi-squared values in tables for ease of viewin

XMM-Newton detection of two clusters of galaxies with strong SPT Sunyaev-Zel'dovich effect signatures

We report on the discovery of two galaxy clusters, SPT-CL J2332-5358 and SPT-CL J2342-5411, in X-rays. These clusters were also independently detected through their Sunyaev-Zel'dovich effect by the South Pole Telescope, and confirmed in the optical band by the Blanco Cosmology Survey. They are thus the first clusters detected under survey conditions by all major cluster search approaches. The X-ray detection is made within the frame of the XMM-BCS cluster survey utilizing a novel XMM-Newton mosaic mode of observations. The present study makes the first scientific use of this operation mode. We estimate the X-ray spectroscopic temperature of SPT-CL J2332-5358 (at redshift z=0.32) to T = 9.3 (+3.3/-1.9) keV, implying a high mass, M_{500} = 8.8 +/- 3.8 \times 10^{14} M_{sun}. For SPT-CL J2342-5411, at z=1.08, the available X-ray data doesn't allow us to directly estimate the temperature with good confidence. However, using our measured luminosity and scaling relations we estimate that T = 4.5 +/- 1.3 keV and M_{500} = 1.9 +/- 0.8 \times 10^{14} M_{sun}. We find a good agreement between the X-ray masses and those estimated from the Sunyaev-Zel'dovich effect.Comment: Submitted to A&A, 8 pages, 5 figures, 1 tabl

Canonical and Microcanonical Distributions for Fermi Systems

Recursion relations are presented that allow exact calculation of canonical and microcanonical partition functions of degenerate Fermi systems, assuming no explicit two-body interactions. Calculations of the level density, sorted by angular momentum, are presented for Ni-56 are presented. The issue of treating unbound states is also addressed.Comment: 5 pages, 5 figure

Isospin Fluctuations from a Thermally Equilibrated Hadron Gas

Partition functions, multiplicity distributions, and isospin fluctuations are calculated for canonical ensembles in which additive quantum numbers as well as total isospin are strictly conserved. When properly accounting for Bose-Einstein symmetrization, the multiplicity distributions of neutral pions in a pion gas are significantly broader as compared to the non-degenerate case. Inclusion of resonances compensates for this broadening effect. Recursion relations are derived which allow calculation of exact results with modest computer time.Comment: 10 pages, 5 figure

Relation between concurrence and Berry phase of an entangled state of two spin 1/2 particles

We have studied here the influence of the Berry phase generated due to a cyclic evolution of an entangled state of two spin 1/2 particles. It is shown that the measure of formation of entanglement is related to the cyclic geometric phase of the individual spins. \\Comment: 6 pages. Accepted in Europhys. Letters (likely to be published in vol 73, pp1-6 (2006)

Is the attentional SNARC effect truly attentional? : Using temporal order judgements to differentiate attention from response

Funding: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Financial support for this study was provided in part by a Natural Science and Engineering Research Council of Canada Discovery Grant (2016-06359) awarded to Jay Pratt. The funding agreement ensured the authors’ independence in designing the study, interpreting the data, writing, and publishing the report.Peer reviewe

First measurements of the flux integral with the NIST-4 watt balance

In early 2014, construction of a new watt balance, named NIST-4, has started at the National Institute of Standards and Technology (NIST). In a watt balance, the gravitational force of an unknown mass is compensated by an electromagnetic force produced by a coil in a magnet system. The electromagnetic force depends on the current in the coil and the magnetic flux integral. Most watt balances feature an additional calibration mode, referred to as velocity mode, which allows one to measure the magnetic flux integral to high precision. In this article we describe first measurements of the flux integral in the new watt balance. We introduce measurement and data analysis techniques to assess the quality of the measurements and the adverse effects of vibrations on the instrument.Comment: 7 pages, 8 figures, accepted for publication in IEEE Trans. Instrum. Meas. This Journal can be found online at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=1