Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid

Vitrification is a new method that has been recently introduced in Assisted Reproduction Technique programs. The aim of this study was to design a new medium similar to normal human seminal fluid (SF), formulation artificial seminal fluid (ASF), and to compare the cryoprotective potency of this medium with SF and human tubal fluid (HTF) medium. Thirty normal ejaculates were processed with the swim-up technique and sperm suspensions were divided into four aliquots: (i) fresh sample (control); (ii) vitrification in HTF medium supplemented with 5 mg/mL human serum albumin and 0.25 mol sucrose (Vit HTF); (iii) vitrification with patients' SF (Vit SF); and (iv) vitrification in ASF (Vit ASF). After warming, sperm parameters of motility, viability, and morphology were analyzed using WHO criteria. Also, sperm pellets were fixed in 2.5% glutaraldehyde and processed for scanning electron microscopy and transmission electron microscopy observations. The results showed that progressive motility (46.09 ± 10.33 vs. 36.80 ± 13.75), grade A motility (36.59 ± 11.40 vs. 16.41 ± 11.24), and normal morphology (18.74 ± 8.35 vs. 11.85 ± 5.84) and viability (68.22 ± 10.83 vs. 60.86 ± 11.72) of spermatozoa were significantly higher in Vit ASF than in Vit HTF. All parameters were better in Vit ASF than in Vit SF, but only viability was significantly different (p = 0.006). After cryopreservation, deep invagination in cytoplasm and mechanically weak point sites and folded tail were commonly observed. But, this phenomenon was more significant in Vit HTF and Vit SF than in ASF (p < 0.05). In transmission electron microscopy evaluation, acrosome damage, plasma membrane loss, chromatin vacuolation, and disruption of mitochondria arrangement and structures were observed in all vitrified groups. Adherence of several tail sections together was also seen in all cryo groups. But this was seen more in Vit HTF and Vit SF than in ASF (p < 0.05). In conclusion, vitrification of human spermatozoa with ASF can effectively preserve the quality of sperm motility in comparison with Vit HTF. © 2016 American Society of Andrology and European Academy of Andrology

An elderly man presenting with an acute upper gastrointestinal bleed: a case report

An 80 year old man presented to the Accident and Emergency Department complaining of “black stools”, increasing shortness of breath, chest tightness and epigastric pain. An upper gastro-intestinal bleed was diagnosed and the patient was managed conservatively with aggressive resuscitation and close monitoring. An oesophogastroduodenoscopy found no cause for the bleeding which ceased and the patient was discharged with a general practitioner follow-up

OpenCL Actors - Adding Data Parallelism to Actor-based Programming with CAF

The actor model of computation has been designed for a seamless support of concurrency and distribution. However, it remains unspecific about data parallel program flows, while available processing power of modern many core hardware such as graphics processing units (GPUs) or coprocessors increases the relevance of data parallelism for general-purpose computation. In this work, we introduce OpenCL-enabled actors to the C++ Actor Framework (CAF). This offers a high level interface for accessing any OpenCL device without leaving the actor paradigm. The new type of actor is integrated into the runtime environment of CAF and gives rise to transparent message passing in distributed systems on heterogeneous hardware. Following the actor logic in CAF, OpenCL kernels can be composed while encapsulated in C++ actors, hence operate in a multi-stage fashion on data resident at the GPU. Developers are thus enabled to build complex data parallel programs from primitives without leaving the actor paradigm, nor sacrificing performance. Our evaluations on commodity GPUs, an Nvidia TESLA, and an Intel PHI reveal the expected linear scaling behavior when offloading larger workloads. For sub-second duties, the efficiency of offloading was found to largely differ between devices. Moreover, our findings indicate a negligible overhead over programming with the native OpenCL API.Comment: 28 page

Equality of averaged and quenched large deviations for random walks in random environments in dimensions four and higher

We consider large deviations for nearest-neighbor random walk in a uniformly elliptic i.i.d. environment. It is easy to see that the quenched and the averaged rate functions are not identically equal. When the dimension is at least four and Sznitman's transience condition (T) is satisfied, we prove that these rate functions are finite and equal on a closed set whose interior contains every nonzero velocity at which the rate functions vanish.Comment: 17 pages. Minor revision. In particular, note the change in the title of the paper. To appear in Probability Theory and Related Fields

Near-field properties of plasmonic nanostructures with high aspect ratio

Using the Green's dyad technique based on cuboidal meshing, we compute the electromagnetic field scattered by metal nanorods with high aspect ratio. We investigate the effect of the meshing shape on the numerical simulations. We observe that discretizing the object with cells with aspect ratios similar to the object's aspect ratio improves the computations, without degrading the convergency. We also compare our numerical simulations to finite element method and discuss further possible improvements

Dynamic Voltage Scaling Techniques for Energy Efficient Synchronized Sensor Network Design

Building energy-efficient systems is one of the principal challenges in wireless sensor networks. Dynamic voltage scaling (DVS), a technique to reduce energy consumption by varying the CPU frequency on the fly, has been widely used in other settings to accomplish this goal. In this paper, we show that changing the CPU frequency can affect timekeeping functionality of some sensor platforms. This phenomenon can cause an unacceptable loss of time synchronization in networks that require tight synchrony over extended periods, thus preventing all existing DVS techniques from being applied. We present a method for reducing energy consumption in sensor networks via DVS, while minimizing the impact of CPU frequency switching on time synchronization. The system is implemented and evaluated on a network of 11 Imote2 sensors mounted on a truss bridge and running a high-fidelity continuous structural health monitoring application. Experimental measurements confirm that the algorithm significantly reduces network energy consumption over the same network that does not use DVS, while requiring significantly fewer re-synchronization actions than a classic DVS algorithm.unpublishedis peer reviewe

Economic development effects of major and minor league teams and stadia

Professional teams and leagues claim new stadiums lead to economic development. To test this, we utilize data from the Census Bureau on net establishment and employment changes across 871 Metropolitan and Micropolitan Statistical Areas from 2004 to 2012. Difference-in-differences and panel data techniques allow for a cross-sectional and time series comparison for both teams and new stadia in both professional and development leagues. Nearly all results from hundreds of models are insignificantly different from zero. Results from between- and random-effects models suggest that teams move into markets that already have higher employment and establishment growth. (JEL R58, H71, L83, Z28