PHYSIOCHEMICAL INVESTIGATION AND BIOVALUATION OF TiO2 NANOCRYSTALS SYNTHESIZED BY CHEMICAL AND GREEN ROUTE

Objectives: In this present work, we have compared titanium dioxide nanocrystals synthesized by both chemical method and biological method from Titanium tetra isopropoxide as precursor. The biological method was performed by using the aqueous root extract of Desmodium gangeticum and further they were characterized and checked for their biological activities. Methods: The TiO2 nanocrystals were characterized by UV-Visible spectrophotometer for its maximum SPR peak, X-ray diffractometry for its crystalline size and shape, Fourier transform infrared spectroscopy for functional entity identification and Zeta potential for its colloidal stability. Antioxidant assay and antimicrobial test were performed to evaluate its biological behaviour. Results: The nanocrystals synthesized by biological method were found to have higher antioxidant potential, antimicrobial activity and phenolic content than the chemically synthesized ones. Evaluation of toxicity in cell line LLC-PK1 suggests that TiO2nanocrystals synthesized by green route is less toxic. Conclusion: Green synthesized TiO2 nanocrystals were seen to have less toxicity than the chemical method in showing their biocompatibility nature which can be applied in the field of medical science

DESMODIUM GANGETICUM ROOT AQUEOUS EXTRACT MEDIATED SYNTHESIS OF NI NANOPARTICLE AND ITS BIOLOGICAL EVALUATION

Objective: A novel, eco-friendly method of Nickel nanoparticles synthesis using aqueous extract of Desmodium gangeticum root (DG) has been reported in this study.Methods: Novel approach on synthesis of Ni nanoparticles using NiCl2 as precursor and aqueous extract of Desmodium gangeticum root as the reducing agent. Nanoparticles were characterized for its average size, morphology, functional moieties and thermal stability by UV-Visible spectrophotometry, X-Ray Diffractometry (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR) and Thermo Gravimetric Analysis (TGA) respectively. Cytotoxicity was also determined against LLC PK1 cell lines using LDH assay.Results: Less aggregate spherical shaped and mono-dispersed nanoparticles were synthesised whose size ranges from 20-30 nm in diameter. Nanoparticles were exhibit face centre cubic crystalline phase with an average size of ~23 nm which was obtained from XRD spectral pattern. Strong interaction between Desmodium gangeticum and nanoparticles was shown in TGA-thermogram. The reducing potential and total phenolic content of Ni nanoparticles was found to be same as that of Desmodium gangeticum. All the results were expressed as mean±SD of n = 4-6 independent assays, p<0.05, whose data were analysed using ANOVA.Conclusion: Biological activity of the nanoparticles and its toxicity was assessed and found to possess the good antioxidant and reduction potential with significant antibacterial activity and were nontoxic.Â

Recurrent Zoonotic Transmission of Nipah Virus into Humans, Bangladesh, 2001–2007

More than half of identified cases result from person-to-person transmission

ONE STEP SYNTHESIS OF IRON OXIDE NANOPARTICLES VIA CHEMICAL AND GREEN ROUTE–AN EFFECTIVE COMPARISON

Objective: Magnetic Iron oxide nanoparticles (IONP) are of potential use in the field of biomedical, bioengineering particularly in vivo applications like tissue repair, drug delivery. However, biocompatibility of the nanoparticles is of great concern. Hence in this manuscript, we compare suitability using IONP prepared by two different routes namely chemical and green, for the biological applications.Methods: In the green route, Desmodium gangeticum root extract was used as the reducing agent with no specific capping agent for the synthesis of nanoparticles unlike chemical route, where propylene glycol was used. The synthesised nanoparticles were characterized and compared by UV-Vis spectrophotometry, X-ray Diffractometry (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Zeta analyser and Vibrating Sample Magnetometry (VSM).Results: The results were similar except for that the size of green synthesised IONP was reduced and possesses even distribution (i.e. mono dispersed). Biological activity, as assessed by its free-radical scavenging potential and anti-microbial effect was found to be better in the case of green IONP. Toxicity studies using LLC-PK1 cell line shows relatively low toxicity of green synthesised nanoparticles.Conclusion: Biologically synthesized IONPshow significant antioxidant effect,retains magnetic behaviour and found to be less toxic, therby proving its compatible nature as required in biomedical applications.Â

SYNTHESIS OF COPPER OXIDE NANOPARTICLES USING DESMODIUM GANGETICUM AQUEOUS ROOT EXTRACT

Objective: In this present work, we have synthesized Copper oxide nanoparticles using Desmodium gangeticum root extracts and explored its biological activity.Methods: The Copper oxide nanoparticles were synthesized by the reduction process. Copper oxide nanoparticles were purified and dried. Scanning electron micrograph analysis showed evenly distributed Copper oxide nanoparticles and was confirmed by UV visible spectroscopy. Characterization of Copper oxide nanoparticles was done with Fourier Transform Infra-Red spectroscopy (FTIR) and Thermo Gravimetric Analysis (TGA). Gram negative and gram positive, bacteria were used to evaluate its antibacterial activity.Results: Reducing potential and free radical scavenging ability of Copper oxide nanoparticles was measured and found to be high. Indeed, toxicological evaluation in LLC-PK1 cell line suggests that Copper oxide nanoparticles were safer for biomedical applications.Conclusion: Biologically synthesized copper oxide nanoparticles show significant antioxidant effect and found to be less toxic as compared to the precursor thereby can be used as potential candidate species for various biomedical applications.Â

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF IRON OXIDE NANOPARTICLES PREPARED BY DESMODIUM GANGETICUM ROOT AQUEOUS EXTRACT

Objective: Synthesis, characterization and biological evaluation of iron oxide nanoparticles prepared by Desmodium gangeticum root aqueous extract. Methods: Iron Oxide nanoparticles (FeO NPs) were synthesized by the reduction of iron chlorides using phyto-constituents as bio-reductants. The synthesis of FeO NPs was confirmed by UV-Visible spectrophotometry and the average particle size was determined by X-ray diffractometry (XRD). Morphology of the synthesized FeO NPs was identified using Scanning Electron Microscopy (SEM). Further characterization of FeO NPs was done to study its nature by FTIR and TGA. Biological activity of FeO NPs was analyzed through its free radical scavenging activity and antimicrobial effect. Further, cytotoxicity was examined against LLC-PK1 cell lines using LDH assay.Results: Thus synthesized particles exhibit rhombohedra crystalline phase. Morphology was identified as spherical and particle size ranges from 25 to 35 nm in diameter with an average size of 30 nm analyzed from SEM micrograph. FeO NPs showed a comparable antimicrobial activity as that of control drug (ciprofloxacin) towards Gram positive bacteria. Iron oxide nanoparticles were also found to be non-toxic by the LDH assayConclusion: Green synthesis of Fe nanoparticles using Desmodium gangeticum root aqueous extract was considered to be relatively safe and particles possesses significant biological activity.Â

GREEN AND CHEMICALLY SYNTHESIZED COPPER OXIDE NANOPARTICLES-A PRELIMINARY RESEARCH TOWARDS ITS TOXIC BEHAVIOUR

Objectives: Metal oxide nanoparticles have been widely explored in various applications like biosensors, solar cells, biomedical applications in the recent times. However, biological applications of these nanoparticles needs to be low compatible and this is often found as a limitation. The present study aims to compare CuO nanoparticles prepared by chemical and green route to evaluate its suitability for biological applications.Methods: CuO nanoparticles prepared by both methods were characterized by using XRD, UV-Visible spectroscopy, FTIR, ZETA for phase structure, size, the functional group presence, thermal stability and surface charge stability respectively. Free radical scavenging potential and phenolic contents, was assessed for biologically prepared CuO nanoparticles.Results: Green synthesized CuO nanoparticles were found to have higher free radical scavenging potential with good colloidal stability and was also found to have well defined monodisperse nature as compared to chemically synthesized CuO nanoparticles. Evaluation of toxicity in cell line LLC PK1 was found to be slightly toxic.Conclusion: Green synthesized CuO nanoparticles was found to have good stability when compared to chemically synthesized ones showing that they possess desired attributes to be used for biomedical applications.Â