Role of rodents in transmission of Salmonella and Campylobacter

Salmonella and Campylobacter are generally regarded as the most important food-borne pathogens in the world. Reduction or elimination of these pathogens in the first part of the food chain (on the farm) is important to prevent disease among consumers of animal products. In organic farming, elimination becomes more difficult, as food animals are allowed outdoors and have easy access to potential sources of hazardous pathogens. Whilst rodents are often associated by organic farmers with infrastructural damage and eating or spoiling of stored feed and products, their zoonotic risks are frequently underestimated. They can amplify the number of pathogens in the environment and transfer them to food animals. Thus organic farmers should be aware of the need for rodent control from a food safety perspective. Preferably, rodent control should form an integral part of a total package of hygiene measures to prevent transfer of food-borne pathogens. These should also include e.g. control of wild birds and flies and obligatory disinfection of boots/clothes and equipment for farm workers and visitors

Animal-friendly production systems may cause re-emergence of Toxoplasma gondii

Toxoplasmosis is still one of the most common parasitic infections in the world, although in Europe improvements in hygiene and the introduction of ‘total’ indoor farming in livestock production have rapidly diminished the problem during the past decades. As a result of public dislike, however, introduction of alternative and more acceptable animal-friendly livestock production systems including outdoor access are gaining ground. Potentially these systems can lead to increased prevalence of certain zoonotic diseases, including Toxoplasmosis. To retain prevalence of this disease in humans at current levels, emphasis should be on disease control at farm-level. This article provides an analysis of various risk factors for farm animals to get infected with Toxoplasma gondii. Access of cats to the farm premises, the use of compost and goat whey, and rodent control were identified as possible risk factors that should be addressed. Consumers should be aware of the fact that Toxoplasma infection, besides through meat, can also be caused by the uptake of contaminated water, soil, fruit and vegetables

Spotify tailoring for promoting effectiveness in cross-functional autonomous squads

Organisations tend to tailor agile methods to scale employed practices to have cross-functional autonomous teams while promoting sustainable creative and productive development at a constant pace. Thus, it is important to investigate how organisations tailor agile practices to get the balance right between teams' autonomy and alignment. Spotify model is originally introduced to facilitate the development of music streaming services in a very large-scale project with a Business-to-Consumer (B2C) model. However, developing a large-scale mission-critical project with a Business-to-Business (B2B) model is not essentially supported by the Spotify model. Thus, embracing Spotify model for such projects should be concerned about the question of how Spotify practices are adjusted to promote the effectiveness of cross-functional autonomous squads in a mission-critical project with B2B model? In this paper, we conduct a longitudinal embedded case study, which lasted 21 months during which 14 semi-structured interviews were conducted. The Grounded Theory (GT) is adopted to analyse the collected data. As a result, we identify practices and processes that promote effectiveness in cross-functional autonomous squads, which have never been discussed in terms of Spotify model before. We also present Spotify Tailoring by highlighting modified and newly introduced practices by the organisation in which the case study was conducted

Controlling risks of pathogen transmission by flies on organic pig farms - A review

Fly prevention and control on animal production units is necessary to prevent the transmission of pathogens that could affect animal and human health and the maintenance of good hygiene. Organic farmers are often hesitant to apply insecticides for this purpose because of their farming philosophy. Organic production systems are relatively open as pigs generally have access to the outdoors. Here, we investigate the need for fly control and analyse various possibilities that organic farmers have to reduce the number of flies on their farms. We conclude that although biological control looks promising, more research should be done concerning its side effects. Currently, optimal monitoring and prevention seem to offer the best solution

Analysis of North Sea offshore wind power variability

This paper evaluates, for a 2030 scenario, the impact on onshore power systems in terms of the variability of the power generated by 81 GW of offshore wind farms installed in the North Sea. Meso-scale reanalysis data are used as input for computing the hourly power production for offshore wind farms, and this total production is analyzed to identify the largest aggregated hourly power variations. Based on publicly available information, a simplified representation of the coastal power grid is built for the countries bordering the North Sea. Wind farms less than 60 km from shore are connected radially to the mainland, while the rest are connected to a hypothetical offshore HVDC (High-Voltage Direct Current) power grid, designed such that wind curtailment does not exceed 1% of production. Loads and conventional power plants by technology and associated cost curves are computed for the various national power systems, based on 2030 projections. Using the MATLAB-based MATPOWER toolbox, the hourly optimal power flow for this regional hybrid AC/DC grid is computed for high, low and medium years from the meso-scale database. The largest net load variations are evaluated per market area and related to the extra load-following reserves that may be needed from conventional generators.Parts of this work were funded by Agentschap.NL, the Netherlands, now RVO.nl (Rijksdienst voor Ondernemend Nederland [25], under the project North Sea Transnational Grid (NSTG). The NSTG project is a cooperation between Delft University of Technology and the Energy Research Center of the Netherlands

Preferred levels for background ducking to produce esthetically pleasing audio for TV with clear speech

In audio production, background ducking facilitates speech intelligibility while allowing the background to fulfill its purpose, e.g., to create ambience, set the mood, or convey semantic cues. Technical details for recommended ducking practices are not currently documented in the literature. Hence, we first analyzed common practices found in TV documentaries. Second, a listening test investigated the preferences of 22 normal-hearing participants on the Loud- ness Difference (LD) between commentary and background during ducking. Highly personal preferences were observed, highlighting the importance of object-based personalization. Sta- tistically significant difference was found between non-expert and expert listeners. On average, non-experts preferred LDs that were 4 LU higher than the ones preferred by experts. A sta- tistically significant difference was also found between Commentary over Music (CoM) and Commentary over Ambience (CoA). Based on the test results, we recommend at least 10 LU difference for CoM and at least 15 LU for CoA. Moreover, a computational method based on the Binaural Distortion-Weighted Glimpse Proportion (BiDWGP) was found to match the median preferred LD for each item with good accuracy (mean absolute error = 1.97 LU ± 2.50)

Immunopathogenesis of canine chronic ulcerative stomatitis.

Canine Chronic Ulcerative Stomatitis is a spontaneously occurring inflammatory disease of the oral mucosa. An immune-mediated pathogenesis is suspected though not yet proven. We have recently reported on the clinical and histologic features, and identification of select leukocyte cell populations within the lesion. A clinical and histologic similarity to oral lichen planus of people was proposed. In the present study, these initial observations are extended by examining lesions from 24 dogs with clinical evidence of chronic ulcerative stomatitis. Because dogs with chronic ulcerative stomatitis often have concurrent periodontal disease, we wondered if dental plaque/biofilm may be a common instigator of inflammation in both lesions. We hypothesized that dogs with chronic ulcerative stomatitis would exhibit a spectrum of pathologic changes and phenotype of infiltrating leukocytes that would inform lesion pathogenesis and that these changes would differ from inflammatory phenotypes in periodontitis. Previously we identified chronic ulcerative stomatitis lesions to be rich in FoxP3+ and IL17+ cells. As such, we suspect that these leukocytes play an important role in lesion pathogenesis. The current study confirms the presence of moderate to large numbers of FoxP3+ T cells and IL17+ cells in all ulcerative stomatitis lesions using confocal immunofluorescence. Interestingly, the majority of IL17+ cells were determined to be non-T cells and IL17+ cell frequencies were negatively correlated with severity on the clinical scoring system. Three histologic subtypes of ulcerative stomatitis were determined; lichenoid, deep stomatitis and granulomatous. Periodontitis lesions, like stomatitis lesions, were B cell and plasma cell rich, but otherwise differed from the stomatitis lesions. Direct immunofluorescence results did not support an autoantibody-mediated autoimmune disease process. This investigation contributes to the body of literature regarding leukocyte involvement in canine idiopathic inflammatory disease pathogenesis

The small quinolone derived compound HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo.

HT61 is a small quinolone-derived compound previously demonstrated to exhibit bactericidal activity against gram-positive bacteria including methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). When combined with the classical antibiotics and antiseptics neomycin, gentamicin, mupirocin and chlorhexidine, HT61 demonstrated synergistic bactericidal activity against both MSSA and MRSA infections in vitro. In this study, we investigated the individual antimicrobial activity of HT61 alongside its capability to increase the efficacy of tobramycin against both a tobramycin sensitive laboratory reference strain (PAO1) and tobramycin resistant clinical isolates (RP73, NN2) of the gram-negative bacteria Pseudomonas aeruginosa (P. aeruginosa). Using broth microdilution methods, the MICs of HT61 against all strains were assessed, as well as the effect of HT61 in combination with tobramycin using both the chequerboard method and bacterial time-kill assays. A murine model of pulmonary infection was also used to evaluate the combination therapy of tobramycin and HT61 in vivo. In these studies, we demonstrated significant synergism between HT61 and Tobramycin against the tobramycin resistant P. aeruginosa strains RP73 and NN2, whilst an additive/intermediate effect was observed for P. aeruginosa strain PA01 which was further confirmed using bacterial time kill analysis. In addition, the enhancement of tobramycin by HT61 was also evident in in vitro assays of biofilm eradication. Finally, in vivo studies revealed analogous effects to those observed in vitro with HT61 when administered in combination with tobramycin against each of the three P. aeruginosa strains at the highest tested dose (10 mg/kg)

A note on the index of closed minimal hypersurfaces of flat tori

Generalizing earlier work by Ros in ambient dimension three, we prove an affine lower bound for the Morse index of closed minimal hypersurfaces inside a flat torus in terms of their first Betti number (with purely dimensional coefficients)