Evaluate the compressive strength of cement paste modified with high reactivity attapulgite and affected by curing temperature

This study explores the influence of partial replacement of high reactivity Attapulgite (HRA) with cement by weight and evaluate the effect of curing temperatures on the compressive strength of modified cement paste (MCP). Recently, the Iraqi clay(Attapulgite) has been processed to pozzolanic material HRA, after extracting it from the quarry the clays crushed and grind to filler then specific the suitable calcinations temperature to make this clays as a pozolznic material. The possibility of replacing the Iraqi clays with cement can be reduce the cost and the impact of cement manufacturing on environment. In this study, three percentages of high reactivity Attapulgite used as a replacement 0, 10 and 20 % by weight of cement. The samples cured in four temperatures 20, 40, 60 and 80 °C. The samples with dimension 50x50x50 mm3 were casted and tested at ages 7, and 28 days. The test result shows that the compressive strength at early ages without HRA and cured at 20 °C were higher than samples with HRA. The maximum percentage of HRA as a replacement with cement has a reduced the compressive strength of the concrete. The result at 28 days for the samples with 10% of HRA at curing temperature 40 °C shows increased in compressive strength up to 60 °C, while when the samples were cured at curing temperature 80 °C shows decreased the compressive strength

Environmental sustainability practices among palm oil millers

Considering the global palm oil production, it can be identified as the second largest vegetable oil. Palm oil is a natural resource that is favorable for the Malaysian climate. In 2017, Malaysia had a total of 454 palm oil mills with a production capacity of approximately 112 million tonnes of fresh fruit bunches. A sustainable environment denotes high income, value addition and zero waste. Nonetheless, palm oil mills are being associated with the discharge of untreated effluent water stream pollution, solid wastes, air pollution, etc. The important objective of this study is to measure the level of sustainable environmental practices parameters and awareness of millers. The primary data were collected through questionnaire survey and interviews from 71 millers in Malaysia. This study used confirmatory factor analysis to describe the relationships between the environmental parameters for measuring environment sustainability. This study found that most of the millers employ positive practices for environmental sustainability, and the highest environment parameter is disposal of solid wastes. However, this study can be implemented in Malaysian palm oil mills for identifying the lowest parameters. This study suggested to the industries that the new national sustainable policies for palm oil mills, especially for small and medium players, may enhance the environmental parameters

Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes

Context: In this study, we aimed to identify the determinants of mitochondrial dysfunction in skeletal muscle (SKLM) of subjects with type 2 diabetes (T2DM), and to evaluate the effect of pioglitazone (PIO) on SKLM mitochondrial proteome. Methods: Two different groups of adults were studied. Group I consisted of 8 individuals with normal glucose tolerance (NGT) and 8 with T2DM, subjected to SKLM mitochondrial proteome analysis by 2D-gel electrophoresis followed by mass spectrometry-based protein identification. Group II included 24 individuals with NGT and 24 with T2DM, whose SKLM biopsies were subjected to immunoblot analysis. Of the 24 subjects with T2DM, 20 were randomized to receive placebo or PIO (15 mg daily) for 6 months. After 6 months of treatment, SKLM biopsy was repeated. Results: Mitochondrial proteomic analysis on Group I revealed that several mitochondrial proteins involved in oxidative metabolism were differentially expressed between T2DM and NGT groups, with a downregulation of ATP synthase alpha chain (ATP5A), electron transfer flavoprotein alpha-subunit (ETFA), cytochrome c oxidase subunit VIb isoform 1 (CX6B1), pyruvate dehydrogenase protein X component (ODPX), dihydrolipoamide dehydrogenase (DLDH), dihydrolipoamide-S-succinyltransferase (DLST), and mitofilin, and an up-regulation of hydroxyacyl-CoA-dehydrogenase (HCDH), 3,2-trans-enoyl-CoA-isomerase (D3D2) and delta3,5-delta2,4-dienoyl-CoA-isomerase (ECH1) in T2DM as compared to NGT subjects. By immunoblot analysis on SKLM lysates obtained from Group II we confirmed that, in comparison to NGT subjects, those with T2DM exhibited lower protein levels of ATP5A (−30%, P = 0.006), ETFA (−50%, P = 0.02), CX6B1 (−30%, P = 0.03), key factors for ATP biosynthesis, and of the structural protein mitofilin (−30%, P = 0.01). T2DM was associated with a reduced abundance of the enzymes involved in the Krebs cycle DLST and ODPX (−20%, P ≤ 0.05) and increased levels of HCDH and ECH1, enzymes implicated in the fatty acid catabolism (+30%, P ≤ 0.05). In subjects with type 2 diabetes treated with PIO for 6 months we found a restored SKLM protein abundance of ATP5A, ETFA, CX6B1, and mitofilin. Moreover, protein levels of HCDH and ECH1 were reduced by −10% and − 15% respectively (P ≤ 0.05 for both) after PIO treatment. Conclusion: Type 2 diabetes is associated with reduced levels of mitochondrial proteins involved in oxidative phosphorylation and an increased abundance of enzymes implicated in fatty acid catabolism in SKLM. PIO treatment is able to improve SKLM mitochondrial proteomic profile in subjects with T2DM

Lentiviral Engineered Fibroblasts Expressing Codon Optimized COL7A1 Restore Anchoring Fibrils in RDEB

Cells therapies, engineered to secrete replacement proteins, are being developed to ameliorate otherwise debilitating diseases. Recessive dystrophic epidermolysis bullosa (RDEB) is caused by defects of type VII collagen (C7), a protein essential for anchoring fibril formation at the dermal-epidermal junction (DEJ). Whilst allogeneic fibroblasts injected directly into the dermis can mediate transient disease modulation, autologous gene-modified fibroblasts should evade immunological rejection and support sustained delivery of C7 at the DEJ. We demonstrate the feasibility of such an approach using a therapeutic grade, self-inactivating-lentiviral vector, encoding codon optimized COL7A1, to transduce RDEB fibroblasts under conditions suitable for clinical application. Expression and secretion of C7 was confirmed, with transduced cells exhibiting supra-normal levels of protein expression and ex vivo migration of fibroblasts was restored in functional assays. Gene modified RDEB fibroblasts also deposited C7 at the DEJ of human RDEB skin xenografts placed on NOD-scid IL2Rgamma(null) recipients, with reconstruction of human epidermal structure and regeneration of anchoring fibrils at the DEJ. Fibroblast mediated restoration of protein and structural defects in this RDEB model strongly supports proposed therapeutic applications in man

Indices of insulin sensitivity and secretion from a standard liquid meal test in subjects with type 2 diabetes, impaired or normal fasting glucose

<p>Abstract</p> <p>Background</p> <p>To provide an initial evaluation of insulin sensitivity and secretion indices derived from a standard liquid meal tolerance test protocol in subjects with normal (NFG), impaired fasting glucose (IFG) or type 2 diabetes mellitus.</p> <p>Methods</p> <p>Areas under the curve (AUC) for glucose, insulin and C-peptide from pre-meal to 120 min after consumption of a liquid meal were calculated, as were homeostasis model assessments of insulin resistance (HOMA2-IR) and the Matsuda index of insulin sensitivity.</p> <p>Results</p> <p>Subjects with NFG (n = 19), IFG (n = 19), and diabetes (n = 35) had mean ± SEM HOMA2-IR values of 1.0 ± 0.1, 1.6 ± 0.2 and 2.5 ± 0.3 and Matsuda insulin sensitivity index values of 15.6 ± 2.0, 8.8 ± 1.2 and 6.0 ± 0.6, respectively. The log-transformed values for these variables were highly correlated overall and within each fasting glucose category (r = -0.91 to -0.94, all p < 0.001). Values for the product of the insulin/glucose AUC ratio and the Matsuda index, an indicator of the ability of the pancreas to match insulin secretion to the degree of insulin resistance, were 995.6 ± 80.7 (NFG), 684.0 ± 57.3 (IFG) and 188.3 ± 16.1 (diabetes) and discriminated significantly between fasting glucose categories (p < 0.001 for each comparison).</p> <p>Conclusion</p> <p>These results provide initial evidence to support the usefulness of a standard liquid meal tolerance test for evaluation of insulin secretion and sensitivity in clinical and population studies.</p

Cloud-To-Ground Lightning Flashes Under The Influence Of Pollution In Malaysia And Some Countries

The effect of air pollution produced from world war, industrial activities and transportation clearly can change the earth ecology system such as the atmospheric conditions. Base on few studies reported from by researcher in USA, Brazil, Spain and South Korea researcher have proven that the pollution effect can enhance the activity of lightning. Those information above motivate Advanced Multidisciplinary System Technology researcher in UTeM to report the cloud-to-ground lightning flashes in Malaysia the influence of pollution. Particulate Matter (PM10) and Sulfur Dioxide (SO2) concentration were used as gross indicator of Cloud Condensation Nuclei (CCN) and examined in relation to the urban increase in CG lightning activity. PM10 concentration recorded in Subang, Ipoh and Bayan Lepas were 51, 50 and 49μgm/m³ respectively. Meanwhile, SO2 concentration recorded were 8, 7.9, 7.7μgm/m³. The three urban areas have average number of CG lightning flashes of 200, 197 and 184. An analysis related number of CG flashes against pollutants show positive correlation in Malaysia with reading of 0.94. This indicates PM10 and SO2 concentration have high influential factor in enhancement of CG lightning activity in Malaysia

Cardiovascular Disease Mortality in Europeans in Relation to Fasting and 2-h Plasma Glucose Levels Within a Normoglycemic Range

OBJECTIVE - To study mortality in relation to fasting plasma glucose (FPG) and 2-h plasma glucose levels within the normoglycemic range