Establishing The Equipment-methodical Support For Determining The Properties Of Extracts Of Grape Pomace Extracts Produced In The Subcreative Water Environment

Research objective: development of a high-pressure reactor for researching the process of extraction of grape pomace by the subcritical water and determining the parameters, providing the maximum yield of various target products – biologically active substances; formation of methodological support for raw material preparation, qualitative and quantitative analysis of extracts, produced by the subcritical extraction. As a result of simulation in the ANSYS system of the stress-strain state of the walls of the reactor chamber and a set of calculation operations, a high-pressure reactor was created that meets the requirements. The formed methodical complex for determining the physicochemical properties of extracts and the content of various biologically active substances included methods for preparing samples and determining the yield of dry extractive substances, evaluation of extraction of polyphenols (tannic-catechol complex), evaluation of extraction of reducing substances, identification furfural and gallic acids, estimation of free organic acids in terms of tartaric acid, evaluation of antioxidant activity of extracts). This methodological complex allows us to estimate the physico-chemical properties of the extracted biologically active substances

Four-dimensional integration by parts with differential renormalization as a method of evaluation of Feynman diagrams

It is shown how strictly four-dimensional integration by parts combined with differential renormalization and its infrared analogue can be applied for calculation of Feynman diagrams.Comment: 6 pages, late

Exponential Renormalization II: Bogoliubov's R-operation and momentum subtraction schemes

This article aims at advancing the recently introduced exponential method for renormalisation in perturbative quantum field theory. It is shown that this new procedure provides a meaningful recursive scheme in the context of the algebraic and group theoretical approach to renormalisation. In particular, we describe in detail a Hopf algebraic formulation of Bogoliubov's classical R-operation and counterterm recursion in the context of momentum subtraction schemes. This approach allows us to propose an algebraic classification of different subtraction schemes. Our results shed light on the peculiar algebraic role played by the degrees of Taylor jet expansions, especially the notion of minimal subtraction and oversubtractions.Comment: revised versio

AKMEOLOGICHESKOJ CONCEPTION OF SCIENTIFIC EDUCATION STUDENTS

The potential of acme approach and the possibility of using it in developing students’ research system are observed. It is supposed that the methods of students’ motivation, of their abilities, competence and independence development in the research process can be analyzed from the acmeological point of view.Рассмотрен потенциал и обоснована возможность применения акмеологического подхода к развитию научной подготовки студентов, в частности, методов стимулирования мотивации студентов к научной деятельности и формирования у них разносторонних способностей в процессе деятельности, воспитания самостоятельной личности в процессе научной деятельности

OPE coefficient functions in terms of composite operators only. Singlet case

A method for calculating coefficient functions of the operator product expansion, which was previously derived for the non-singlet case, is generalized for the singlet coefficient functions. The resulting formula defines coefficient functions entirely in terms of corresponding singlet composite operators without applying to elementary (quark and gluon) fields. Both "diagonal" and "non-diagonal" gluon coefficient functions in the product expansion of two electromagnetic currents are calculated in QCD. Their renormalization properties are studied.Comment: 33 pages, 15 figures, minor corrections are mad

YopN and TyeA Hydrophobic Contacts Required for Regulating Ysc-Yop Type III Secretion Activity by Yersinia pseudotuberculosis

Yersinia bacteria target Yop effector toxins to the interior of host immune cells by the Ysc-Yop type III secretion system. A YopN-TyeA heterodimer is central to controlling Ysc-Yop targeting activity. A + 1 frameshift event in the 3-prime end of yopN can also produce a singular secreted YopN-TyeA polypeptide that retains some regulatory function even though the C-terminal coding sequence of this YopN differs greatly from wild type. Thus, this YopN C-terminal segment was analyzed for its role in type III secretion control. Bacteria producing YopN truncated after residue 278, or with altered sequence between residues 279 and 287, had lost type III secretion control and function. In contrast, YopN variants with manipulated sequence beyond residue 287 maintained full control and function. Scrutiny of the YopN-TyeA complex structure revealed that residue W 279 functioned as a likely hydrophobic contact site with TyeA. Indeed, a YopN W 279 G mutant lost all ability to bind TyeA. The TyeA residue F 8 was also critical for reciprocal YopN binding. Thus, we conclude that specific hydrophobic contacts between opposing YopN and TyeA termini establishes a complex needed for regulating Ysc-Yop activity

Operator product expansion coefficient functions in terms of composite operators only. Nonsinglet case

A new method for calculating the coefficient functions of the operator product expansion is proposed which does not depend explicitly on elementary fields. Coefficient functions are defined entirely in terms of composite operators. The method is illustrated in the case of QCD nonsinglet operators.Comment: Derivation of the main formula is improved. References are added. To appear in Physical Review

Methylation, crystallization and SAD phasing of the Csu pilus CsuC-CsuE chaperone-adhesin subunit pre-assembly complex from Acinetobacter baumannii

Acinetobacter baumannii is one of the most difficult Gram-negative bacteria to control and treat. This pathogen forms biofilms on hospital surfaces and medical devices using Csu pili assembled via the archaic chaperone-usher pathway. To uncover the mechanism of bacterial attachment to abiotic surfaces, it was aimed to determine the crystal structure of the pilus tip adhesin CsuE. The CsuC-CsuE chaperone-subunit pre-assembly complex was purified from the periplasm of Escherichia coli overexpressing CsuC and CsuE. Despite the high purity of the complex, no crystals could be obtained. This challenge was solved by the methylation of lysine residues. The complex was crystallized in 0.1 M bis-tris pH 5.5, 17% PEG 3350 using the hanging-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.31 angstrom and belonged to the triclinic space group P1, with unit-cell parameters a = 53.84, b = 63.85, c = 89.25 angstrom, alpha = 74.65, beta = 79.65, gamma = 69.07 degrees. Initial phases were derived from a single anomalous diffraction experiment using a selenomethionine derivative

Zero-mode contribution to the light-front Hamiltonian of Yukawa type models

Light-front Hamiltonian for Yukawa type models is determined without the framework of canonical light-front formalism. Special attention is given to the contribution of zero modes.Comment: 14 pages, Latex, revised version with minor changes, Submitted to J.Phys.