Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line.

The association between T3 and the T cell antigen receptor was examined using the T3 bearing T cell leukemic line Jurkat. A monoclonal antibody, C305, was produced, which reacted with idiotypic-like determinants expressed on Jurkat. The molecule with which this antibody reacted was a disulfide-linked heterodimer of 90 kD, composed of polypeptides of 42 and 54 kD. Thus, C305 reacted with a molecule with characteristics of the putative T cell antigen receptor described by others. A series of mutants of Jurkat, induced with ethyl methane sulfonate or radiation, was selected for T3 or antigen receptor negativity. In every instance, there was a concomitant loss of both T3 and the antigen receptor as assessed by quantitative absorption, indirect immunofluorescence, and antibody plus complement-mediated cytotoxicity. The absence of antigen receptor molecules was confirmed on diagonal gels, excluding the possibility that conformational changes of the antigen receptor on such T3-negative mutants were responsible for the failure of such mutants to react with C305. Moreover, in a mutant that expressed a marked decrease in the level of T3 expression, there was a comparable decrease in the expression of antigen receptor determinants. These results suggest that there is an obligate requirement for the coexpression of T3 and the T cell antigen receptor. Furthermore, attempts to activate such mutants with the lectin phytohemagglutinin suggested that the expression of T3 and/or the antigen receptor was required for activation of these cells

Resonant-state expansion for open optical systems: Generalization to magnetic, chiral, and bi-anisotropic materials

The resonant-state expansion, a recently developed powerful method in electrodynamics, is generalized here for open optical systems containing magnetic, chiral, or bi-anisotropic materials. It is shown that the key matrix eigenvalue equation of the method remains the same, but the matrix elements of the perturbation now contain variations of the permittivity, permeability, and bi-anisotropy tensors. A general normalization of resonant states in terms of the electric and magnetic fields is presented.Comment: 4 page

Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3.

The T cell antigen receptor consists of the Ti alpha/beta heterodimer which recognizes antigen, and the associated CD3 chains, thought to be involved in signal transduction. To understand the nature of the interaction between Ti and CD3, chimeric molecules which included the COOH-terminal segments of Ti alpha or beta linked to the extracellular segment of CD8, were transfected into a mutant T cell deficient in Ti beta chain expression and cell surface CD3. Both chimeric chains were required to express the chimeric Ti and to restore CD3 surface expression. CD8/Ti and CD3 cointernalized and coimmunoprecipitated. Stimulation of the chimeric receptor induced transmembrane signaling events and cell activation. These results demonstrate that the Ti alpha and beta COOH termini containing the transmembrane domains are sufficient for structural and functional coupling of Ti to CD3

Phosphotyrosines in the killer cell inhibitory receptor motif of NKB1 are required for negative signaling and for association with protein tyrosine phosphatase 1C.

NKB1 is one member of a growing family of killer cell inhibitory receptors (KIR). It is expressed on natural killer (NK) cells and T cells, and has been shown to inhibit cytolytic functions of these cells upon interacting with its ligand, HLA-B (Bw4). We demonstrate here that the cytoplasmic region of NKB1 is capable of inhibiting T cell activation in Jurkat cells. The tyrosine phosphorylation of the NKB1 KIR consensus motif, YxxL(x)26 YxxL, induces an association with the protein tyrosine phosphatase 1C (PTP1C). Importantly, mutation of both tyrosines in the motif abolished the inhibitory functions of NKB1 and abrogated PTP1C association. Mutational analysis of the individual tyrosines suggest that the membrane proximal tyrosine may play a crucial role in mediating the inhibitory signal. These results demonstrate that KIR can not only inhibit cytolytic activity, but can also negatively regulate T cell receptor activation events that lead to downstream gene activation, and further supports a model that implicates PTP1C as a mediator in the KIR inhibitory signal

Gate-tunable split Kondo effect in a carbon nanotube quantum dot

We show a detailed investigation of the split Kondo effect in a carbon nanotube quantum dot with multiple gate electrodes. It is found that the splitting decreases for increasing magnetic field, to result in a recovered zero-bias Kondo resonance at finite magnetic field. Surprisingly, in the same charge state, but under different gate-configurations, the splitting does not disappear for any value of the magnetic field, but we observe an avoided crossing of two high-conductance lines. We think that our observations can be understood in terms of a two-impurity Kondo effect with two spins coupled antiferromagnetically. The exchange coupling between the two spins can be influenced by a local gate, and the non-recovery of the Kondo resonance for certain gate configurations is explained by the existence of a small antisymmetric contribution to the exchange interaction between the two spins.Comment: 12 pages, 4 figures, published versio

Low-luminosity Extragalactic Water Masers toward M82, M51, and NGC4051

Sub-arcsecond observations using the Very Large Array (VLA) are presented for low-luminosity water maser in M82, M51, and NGC4051. New maser features have been detected within the M82 starburst complex. They are largely associated with star-forming activity, such as optically identified starburst-driven winds, H II regions, or the early phase of star formation in the galaxy. The water maser in M51 consists of blueshifted and redshifted features relative to thesystemic velocity of the galaxy. The redshifted features are measured to the northwest of the nuclear radio source, while the location of the blueshifted counterpart is displaced by about 2" from the radio source. A small velocity gradient closely aligned with the radio jet is detected from the redshifted features. The redshifted maser most likely amplifies the background radio continuum jet, while the blueshifted counterpart marks off-nuclear star formation in the galaxy. All of the detected maser features in the narrow-line Seyfert 1 galaxy NGC 4051 remain unresolved by new VLA observations. Due to the low luminosity of the maser, the maser excitation is not directly related to the active galactic nucleus.Comment: 20 pages, 10 figures. Accepted for publication in AJ (17/Oct/2006