Optimized methods to measure acetoacetate, 3-hydroxybutyrate, glycerol, alanine, pyruvate, lactate and glucose in human blood using a centrifugal analyser with a fluorimetric attachment

Optimized methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, D-3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood using the Cobas Bio centrifugal analyser. Glucose and lactate are measured using the photometric mode and other metabolites using the fluorimetric mode. The intra-assay coefficients of variation ranged from 0.7 to 4.1%, except with very low levels of pyruvate and acetoacetate where the coefficients of variation were 7.1 and 12% respectively. All seven metabolites can be measured in a perchloric acid extract of 20 μl of blood. The methods have been optimized with regard to variation in the perchloric acid content of the samples. These variations arise from the method of sample preparation used to minimize changes occurring in metabolite concentration after venepuncture

SuperWASP: Wide Angle Search for Planets

SuperWASP is a fully robotic, ultra-wide angle survey for planetary transits. Currently under construction, it will consist of 5 cameras, each monitoring a 9.5 x 9.5 deg field of view. The Torus mount and enclosure will be fully automated and linked to a built-in weather station. We aim to begin observations at the beginning of 2003.Comment: 4 pages, 1 figure, to be published in proceedings of "Scientific Frontiers in Research on Extrasolar Planets

Current Status of the SuperWASP Project

We present the current status of the SuperWASP project, a Wide Angle Search for Planets. SuperWASP consists of up to 8 individual cameras using ultra-wide field lenses backed by high-quality passively cooled CCDs. Each camera covers 7.8 x 7.8 sq degrees of sky, for nearly 500 sq degrees of sky coverage. SuperWASP I, located in LaPalma, is currently operational with 5 cameras and is conducting a photometric survey of a large numbers of stars in the magnitude range ~7 to 15. The collaboration has developed a custom-built reduction pipeline and aims to achieve better than 1 percent photometric precision. The pipeline will also produce well sampled light curves for all the stars in each field which will be used to detect: planetary transits, optical transients, and track Near-Earth Objects. Status of current observations, and expected rates of extrasolar planetary detections will be presented. The consortium members, institutions, and further details can be found on the web site at: http://www.superwasp.org.Comment: 3 pages, 2 figures, submitted to the Proceedings of the 13th Cool Stars Workshop, Ed. F. Favata, ESA-S

Impact of Laws Aimed at Healthcare-Associated Infection Reduction: A Qualitative Study

Background: Healthcare-associated infections (HAIs) are preventable. Globally, laws aimed at reducing HAIs have been implemented. In the USA, these laws are at the federal and state levels. It is not known whether the state interventions are more effective than the federal incentives alone. Objective: The aims of this study were to explore the impact federal and state HAI laws have on state departments of health and hospital stakeholders in the USA and to explore similarities and differences in perceptions across states. Methods: A qualitative study was conducted. In 2012, we conducted semistructured interviews with key stakeholders from states with and without state-level laws to gain multiple perspectives. Interviews were transcribed and open coding was conducted. Data were analysed using content analysis and collected until theoretical saturation was achieved. Results: Ninety interviews were conducted with stakeholders from 12 states (6 states with laws and 6 states without laws). We found an increase in state-level collaboration. The publicly reported data helped hospitals benchmark and focus leaders on HAI prevention. There were concerns about the publicly reported data (eg, lack of validation and timeliness). Resource needs were also identified. No major differences were expressed by interviewees from states with and without laws. Conclusions: While we could not tease out the impact of specific interventions, increased collaboration between departments of health and their partners is occurring. Harmonisation of HAI definitions and reporting between state and federal laws would minimise reporting burden. Continued monitoring of the progress of HAI prevention is needed

New periodic variable stars coincident with ROSAT sources discovered using SuperWASP

We present optical lightcurves of 428 periodic variable stars coincident with ROSAT X-ray sources, detected using the first run of the SuperWASP photometric survey. Only 68 of these were previously recognised as periodic variables. A further 30 of these objects are previously known pre-main sequence stars, for which we detect a modulation period for the first time. Amongst the newly identified periodic variables, many appear to be close eclipsing binaries, their X-ray emission is presumably the result of RS CVn type behaviour. Others are probably BY Dra stars, pre-main sequence stars and other rapid rotators displaying enhanced coronal activity. A number of previously catalogued pulsating variables (RR Lyr stars and Cepheids) coincident with X-ray sources are also seen, but we show hat these are likely to be misclassifications. We identify four objects which are probable low mass eclipsing binary stars, based on their very red colour and light curve morphology

Distances, ages, and epoch of formation of globular clusters

We review the results on distances and absolute ages of galactic globular clusters (GCs) obtained after the release of the Hipparcos catalogue. Several methods for the Population II local distance scale are discussed, exploiting NEW RESULTS for RR Lyraes in the Large Magellanic Cloud (LMC). We find that the so-called Short and Long Distance Scales may be reconciled whether a consistent reddening scale is adopted for Cepheids and RR Lyrae variables in the LMC. Distances and ages for the 9 clusters discussed in Paper I are re-derived using an enlarged sample of local subdwarfs, which includes about 90% of the metal-poor dwarfs with accurate parallaxes (Delta p/p < 0.12) in the whole Hipparcos catalogue. On average, our revised distance moduli are decreased by 0.04 mag with respect to Paper I. The corresponding age of the GCs is t=11.5+-2.6 Gyr (95% confidence range). The relation between Mv(ZAHB) and metallicity for the nine programme clusters turns out to be Mv(ZAHB)=(0.18+-0.09)([Fe/H]+1.5)+(0.53+-0.12).Thanks to Hipparcos the major contribution to the total error budget associated with the subdwarf fitting technique has been moved from parallaxes to photometric calibrations, reddening and metallicity scale. This total uncertainty still amounts to about +-0.12 mag. Comparing the corresponding (true) LMC distance modulus 18.64+-0.12 mag with other existing determinations, we conclude that at present the best estimate for the distance of the LMC is: 18.54+-0.03+-0.06, suggesting that distances from the subdwarf fitting method are 1 sigma too long. Consequently, our best estimate for the age of the GCs is revised to: Age = 12.9+-2.9 Gyr (95% confidence range). The best relation between Mv(ZAHB) and [Fe/H] is: Mv(ZAHB) =(0.18+-0.09)([Fe/H]+1.5)+(0.63+-0.07).Comment: 76 pages, 6 encapsulated figures and 6 tables. Latex, uses aasms4.sty. Revised and improved version, with new data on field RR Lyraes in LMC. Accepted in the Astrophysical Journa

The Age Of Globular Clusters In Light Of Hipparcos: Resolving the Age Problem?

We review five independent techniques which are used to set the distance scale to globular clusters, including subdwarf main sequence fitting utilizing the recent Hipparcos parallax catalogue. These data together all indicate that globular clusters are farther away than previously believed, implying a reduction in age estimates. This new distance scale estimate is combined with a detailed numerical Monte Carlo study designed to assess the uncertainty associated with the theoretical age-turnoff luminosity relationship in order to estimate both the absolute age and uncertainty in age of the oldest globular clusters. Our best estimate for the mean age of the oldest globular clusters is now $11.5\pm 1.3$Gyr, with a one-sided, 95% confidence level lower limit of 9.5 Gyr. This represents a systematic shift of over 2 $\sigma$ compared to our earlier estimate, due completely to the new distance scale---which we emphasize is not just due to the Hipparcos data. This now provides a lower limit on the age of the universe which is consistent with either an open universe, or a flat, matter dominated universe (the latter requiring H_0 \le 67 \kmsmpc). Our new study also explicitly quantifies how remaining uncertainties in the distance scale and stellar evolution models translate into uncertainties in the derived globular cluster ages. Simple formulae are provided which can be used to update our age estimate as improved determinations for various quantities become available.Comment: 41 pages, including 10 eps figs, uses aaspp4.sty and flushrt.sty, submitted to Ap.J., revised to incorporate FULL Hipparcos catalogue dat

Optimization of a Morphing Wing Based on Coupled Aerodynamic and Structural Constraints

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77091/1/AIAA-39016-101.pd