Discrimination of a selected set of turmeric, ginger, fenugreek and coriander varieties using ISSR markers

DNA fingerprints are unique to individuals and can be used to identify individuals as in the case of conventional fingerprints. Plant DNA fingerprinting make use of various molecular markers for identifying newly released crop varieties and are all the more important in plant variety registration under the PPV&FR Act of 2001. The trade-related intellectual property rights (TRIPS) and the convention on biological diversity (CBD) insist on the establishment of identity and ownership of genotypes for enforcement of their provisions for securing protection to plant varieties as well as for regulating access to germplasm resources. DNA fingerprints, along with morphological markers, can be efficiently utilized for plant varietal identification, detection of duplicates and adulterants. Here in this particular study, the spice samples received at the DNA fingerprinting facility (DNAFF) of ICAR-Indian Institute of Spices Research (ICAR-IISR) from various centres of All India Coordinated Project on Spices (AICRPS) were DNA fingerprinted using inter simple sequence repeat (ISSR) markers. The DNA profile of a candidate variety vis-a-vis check variety is an essential prerequisite during submission of proposal for release of crop variety to central sub-committee on crop standards notification and release of varieties. The new varieties of turmeric, ginger, coriander and fenugreek were compared with the closely resembling check varieties for establishing distinctness for varietal registration. A total of 118 ISSR primers were screened in the above-given crops, to identify the distinct markers identifying the candidate from the check varieties. Using this technique, the DNAFF at ICAR-IISR could facilitate registration of turmeric varieties, Roma, Rasmi and Suroma; ginger varieties Suruchi, Suravi and Suprabha; coriander varieties, Suguna, Susthira and Suruchi, while varieties of turmeric, Uttara Rupanjana and Uttara Ranjini; fenugreek variety Ajmer fenugreek (AFg-5); coriander varieties Ajmer coriander (ACr-2) and Chhattisgarh Shri Chandra Hasini dhaniya-2 (ICS-4) are in the process of getting registration. ISSR markers were found to be appropriate for establishing distinctness of the new varieties of spices for securing varietal registration

Design and Implementation of Open Journal System (OJS) for Rajagiri Journals: A Review

Open Access (OA) is an alternative business model for the publication of scholarly journals. It makes articles freely available to readers on the Internet and covers the costs associated with publication through means other than subscriptions. Online Journal System (OJS) is an end to end publishing management platform offered by Public Knowledge Project (PKU) which will help Journal publishers and content developers to manage its journal website along with managing pre-publishing editorial activities including manuscript management, peer review process & publishing process. The OJS platform will cover all aspects of online journal publishing, from establishing a journal website to operational tasks such as the author\u27s submission process, peer review, editing, publication, archiving, and indexing of the journal. It also helps to manage the people facets of organizing a journal, including keeping track of the articles, the work done by the editors, reviewers, and authors, notifying readers, and assisting with the communication. In this paper, we try to discuss the practical challenges and way to overcome it which we implemented Rajagiri Journals through OJS platfor

(1Z)-1-(2,4-Dichloro­phen­yl)ethan-1-one semicarbazone

In the title compound, C9H9Cl2N3O, the semicarbazone group is approximately planar, with an r.m.s deviation from the mean plane of 0.011 (2) Å. The dihedral angle between the least-squares planes through the semicarbazone group and the benzene ring is 38.76 (9)°. The crystal structure is further stabilized by N—H⋯O and C—H⋯O hydrogen bonding

Diethyl 4-[5-(4-chloro­phen­yl)-1H-pyrazol-4-yl]-2,6-dimethyl-1,4-dihydro­pyridine-3,5-dicarboxyl­ate

In the title compound, C22H24ClN3O4, intra­molecular C—H⋯O and C—H⋯N hydrogen bonds form S(9) and S(7) ring motifs, respectively. The 1,4-dihydro­pyridine ring adopts a flattened boat conformation. The benzene ring makes a dihedral angle of 33.36 (6)° with the pyrazole ring. In the crystal, pairs of N—H⋯N hydrogen bonds link the mol­ecules into inversion dimers. The dimers are stacked in column along the a axis through N—H⋯O and C—H⋯N hydrogen bonds. The crystal packing also features C—H⋯π inter­actions involving the pyrazole ring

2-(4-Chloro­phen­yl)-2-oxoethyl 3,4-dimeth­oxy­benzoate

In the title compound, C17H15ClO5, the benzene rings forms a dihedral angle of 74.45 (10)°. In the crystal, mol­ecules are linked into C(13) chains along [011] via C—H⋯O hydrogen bonds. The crystal packing also features short Cl⋯Cl contacts of 3.1253 (10) Å

2-(4-Chloro­anilino)-1-(4-chloro­phen­yl)ethanone

In the title compound, C14H11Cl2NO, the benzene rings form a dihedral angle of 3.14 (6)°. Overall, the mol­ecule is close to being planar (r.m.s. deviation for all the non-H atoms = 0.054 Å). No significant directional inter­molecular inter­actions are observed in the crystal structure

Diethyl 4-[2-(4-meth­oxy­phen­yl)-1H-pyrazol-3-yl]-2,6-dimethyl-1,4-dihydro­pyridine-3,5-dicarboxyl­ate

In the title compound, C23H27N3O5, the pyrazole ring is inclined at dihedral angles of 38.16 (6) and 80.80 (6)°, respectively, to the least-squares planes of the benzene and dihydro­pyridine rings. In the crystal, adjacent mol­ecules are linked via a pair of N—H⋯N hydrogen bonds, forming an inversion dimer. The dimers are stacked in a column along the a axis through N—H⋯O hydrogen bonds. Intra- and inter­molecular C—H⋯N and C—H⋯O hydrogen bonds are also observed

Diethyl 4-[5-(biphenyl-4-yl)-1H-pyrazol-4-yl]-2,6-dimethyl-1,4-dihydro­pyridine-3,5-dicarboxyl­ate ethanol monosolvate

In the title compound, C28H29N3O4·C2H6O, the benzene ring makes dihedral angles of 33.72 (13) and 32.86 (13)°, respectively, with the adjacent pyrazole and phenyl rings. In the crystal, the components are connected via inter­molecular N—H⋯O, N—H⋯N, O—H⋯O and C—H⋯O hydrogen bonds, forming a layer parallel to the bc plane

2-Chloro-4-methyl­pyridin-3-amine

Geometric parameters of the title compound, C6H7ClN2, are in the usual ranges. The mol­ecular structure shows one intra­molecular N—H⋯Cl contact and the crystal packing is stabilized by an inter­molecular N—H⋯N hydrogen bond

(2E)-1-(3-Methyl-2-thien­yl)-3-(3-nitro­phen­yl)prop-2-en-1-one

Chalcones are a major class of natural products having inter­esting pharmaceutical activities. The title compound, C14H11NO3S, is of inter­est as a potential bioactive agent. The central acyclic C=C double bond is trans configured. All non-H atoms lie in a common plane (r.m.s. deviation 0.075 Å). In the crystal structure, the mol­ecules form a herringbone pattern