1,438 research outputs found

    Aspects Of Pulmonary Surfactant Metabolism

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    Pulmonary surfactant stabilizes the lung by reducing the surface tension at the air liquid interface of the alveolus. Phosphatidylcholine represents the principal component of surfactant (70% by weight), other lipids account for 20% of surfactant and the remaining 10% is protein. Four surfactant-associated proteins have been identified, surfactant associated protein (SP) SP-A, SP-B, SP-C and SP-D.;This thesis describes the investigations on the following aspects of surfactant metabolism: (1) phosphatidylcholine synthesis, (2) alterations in surfactant after lung transplantation and (3) mechanisms of alveolar surfactant subtype conversion.;(1) The de novo synthesis of acyl-specific phosphatidylcholine via the acylation of glycerol-3-phosphocholine (GPC) has been investigated in lung and liver. Attempts to acylate radioactive GPC were unsuccessful. It appears the previously reported evidence in favour of the GPC dependent pathway was based on inaccurate identification of radioactive products. In liver, glycerol and glucose were identified as the true reaction products. Lung also formed some glycerol and another unidentified product which was shown not to be GPC. (2) In many types of lung injury, respiratory failure is associated with alterations in pulmonary surfactant. Prolonged storage of a donor lung before transplantation leads to respiratory failure after reperfusion. Analysis of lung lavage after transplantation revealed several alterations in surfactant; phosphatidylglycerol was decreased, sphingomyelin was increased, SP-A was decreased and the ratio of large to small surfactant aggregates was increased. Furthermore, the amount of blood proteins in lung lavage was increased. It was concluded that the effects of surfactant supplementation in lung transplantation should be investigated. (3) The large surfactant aggregate subtype, obtained as a 40,000g pellet after centrifugation of lung lavage, can be converted to the small surfactant aggregate subtype by an in vitro method known as surface area cycling. Subtype conversion leads to the degradation of SP-B, as demonstrated by dot blot analysis, resulting in a loss of biophysical activity. It is proposed that this degradation occurs during lipid adsorption initiated by a changing surface area.;Surface area cycling of different surfactant preparations led to the conclusion that SP-A and SP-B are necessary for large aggregate integrity

    Multi-agent collaborative search : an agent-based memetic multi-objective optimization algorithm applied to space trajectory design

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    This article presents an algorithm for multi-objective optimization that blends together a number of heuristics. A population of agents combines heuristics that aim at exploring the search space both globally and in a neighbourhood of each agent. These heuristics are complemented with a combination of a local and global archive. The novel agent-based algorithm is tested at first on a set of standard problems and then on three specific problems in space trajectory design. Its performance is compared against a number of state-of-the-art multi-objective optimization algorithms that use the Pareto dominance as selection criterion: non-dominated sorting genetic algorithm (NSGA-II), Pareto archived evolution strategy (PAES), multiple objective particle swarm optimization (MOPSO), and multiple trajectory search (MTS). The results demonstrate that the agent-based search can identify parts of the Pareto set that the other algorithms were not able to capture. Furthermore, convergence is statistically better although the variance of the results is in some cases higher

    Time-resolved characterization of a pulsed discharge in a stationary bubble

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    In recent years, plasma generation in water has been proposed for the application of water treatment. The process efficiency is believed to be improved by the introduction of bubbles in the plasma active region. For further optimization, the initiating and developmental mechanisms of plasma inside bubbles need to be understood to a greater extent. In order to meet this necessity, we investigated pulsed electrical discharge inside a stationary bubble in water. This paper deals with the evolution of the discharge and of the bubble shape during discharge, investigated by electrical characterization and fast imaging. Only several microseconds after the application of the voltage pulse, plasma light is observed. Different phases are observed during plasma formation. The plasma is strongest at the bubble surface, causing the surrounding water to evaporate. This leads to both the formation of propagating streamers into the water and the expansion and collapse of the bubble. These observations show that plasma inside a bubble has the strongest activity at the bubble surface, making it attractive for water treatment

    Межфазный катализ: синтез гликозильных эфиров N-ацетилглюкозамина

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    В межфазной системе “твердое тело — органический растворитель” в присутствии каталитических количеств 15-краун-5 перацетат α-D-глюкозаминилхлорида легко образует гликозильные эфиры ряда карбоновых кислот. Полученные 1-0-β-ацилпиранозы идентифицированы с помощью ¹Н ЯМР спектроскопии.У міжфазній системі “тверде тіло — органічний розчинник” у присутності каталітичної кількості 15-краун-5 перацетат α-D-глюкозамінілхлориду легко утворює глікозильні естери ряду карбонових кислот. Отримані 1-O-β-ацилпіранози ідентифіковані за допомогою ¹Н ЯМР-спектроскопії.Peracetate of α-D-glucosaminyl chloride forms easily the N-acetylglucosamine glycosyl esters of the carboxylic acids range in the phase transfer system of “solid-organic solvent” in the presence of catalytic amounts of 15-crown-5. The structure of 1-O-β-acylpyranose synthesized was identified by ¹H NMR spectroscopy

    Power laws and self-similar behavior in negative ionization fronts

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    We study anode-directed ionization fronts in curved geometries. When the magnetic effects can be neglected, an electric shielding factor determines the behavior of the electric field and the charged particle densities. From a minimal streamer model, a Burgers type equation which governs the dynamics of the electric shielding factor is obtained. A Lagrangian formulation is then derived to analyze the ionization fronts. Power laws for the velocity and the amplitude of streamer fronts are observed numerically and calculated analytically by using the shielding factor formulation. The phenomenon of geometrical diffusion is explained and clarified, and a universal self-similar asymptotic behavior is derived.Comment: 25 pages, 9 figure

    3-D properties of pulsed corona streamers

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    Properties of pulsed corona streamers are measured and simulated in full three spatial dimensions (3D). Stereo photography is used to measure branching angles and to investigate whether apparent streamers reconnections are real. 3D simulations of two parallel streamers show that they can repel each other electrostatically, but that they also can merge due to photoionization. The electrostatic interaction of several streamers becomes evident through theoretical investigations of a periodic array of streamers

    The Memory Metal Minimal Access Cage: A New Concept in Lumbar Interbody Fusion—A Prospective, Noncomparative Study to Evaluate the Safety and Performance

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    Study Design/Objective. A single-centre, prospective, non-comparative study of 25 patients to evaluate the performance and safety of the Memory Metal Minimal Access Cage (MAC) in Lumbar Interbody Fusion. Summary of Background Data. Interbody fusion cages in general are designed to withstand high axial loads and in the meantime to allow ingrowth of new bone for bony fusion. In many cages the contact area with the endplate is rather large leaving a relatively small contact area for the bone graft with the adjacent host bone. MAC is constructed from the memory metal Nitinol and builds on the concept of sufficient axial support in combination with a large contact area of the graft facilitating bony ingrowth and ease in minimal access implantation due to its high deformability. Methods. Twenty five subjects with a primary diagnosis of disabling back and radicular leg pain from a single level degenerative lumbar disc underwent an interbody fusion using MAC and pedicle screws. Clinical performance was evaluated prospectively over 2 years using the Oswestry Disability Index (ODI), Short Form 36 questionnaire (SF-36) and pain visual analogue scale (VAS) scores. The interbody fusion status was assessed using conventional radiographs and CT scan. Safety of the device was studied by registration of intra- and post-operative adverse effects. Results. Clinical performance improved significantly (P < .0018), CT scan confirmed solid fusion in all 25 patients at two year follow-up. In two patients migration of the cage occurred, which was resolved uneventfully by placing a larger size at the subsequent revision. Conclusions. We conclude that the Memory Metal Minimal Access Cage (MAC) resulted in 100% solid fusions in 2 years and proved to be safe, although two patients required revision surgery in order to achieve solid fusion

    Breakdown in mm-sized discharges : modifying the electric field

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    Due to the small size of the gas gap in micro- and millimeter sized discharges, the presence of a metallic structure in its vicinity profoundly influences the breakdown process. This is a drawback because it makes electrical probing impossible, but can also be an advantage because it allows control over the electric field in the discharge reactor. Optical and electrical measurements were performed in an argon atmosphere at 0.3 or 0.7 bar. A pin-pin geometry was used, with 4 or 7mm between the electrode tips. We found that both active and passive structures influence breakdown, and we demonstrated the differences between the two types and their effects on the breakdown process

    Maternal protein restriction during perinatal life affects lung mechanics and the surfactant system during early postnatal life in female rats.

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    Limited information is available on how fetal growth retardation (FGR) affects the lung in the neonatal period in males and females. This led us to test the hypothesis that FGR alters lung mechanics and the surfactant system during the neonatal period. To test this hypothesis a model of FGR was utilized in which pregnant rat dams were fed a low protein diet during both the gestation and lactation period. We subsequently analyzed lung mechanics using a FlexiVent ventilator in male and female pups at postnatal day 7 and 21. Lung lavage material was obtained at postnatal day 1, 7 and 21, and was used for analysis of the surfactant system which included measurement of the pool size of surfactant and its subfraction as well as the surface tension reducing ability of the surfactant. The main result of the study was a significantly lower lung compliance and higher tissue elastance which was observed in FGR female offspring at day 21 compared to control offspring. In addition, female LP offspring exhibited lower surfactant pool sizes at postnatal day 1compared to controls. These changes were not observed in the male offspring. It is concluded that FGR has a different impact on pulmonary function and on surfactant in female, as compared to male, offspring
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