Radio Astronomical Polarimetry and Point-Source Calibration

A mathematical framework is presented for use in the experimental determination of the polarimetric response of observatory instrumentation. Elementary principles of linear algebra are applied to model the full matrix description of the polarization measurement equation by least-squares estimation of non-linear, scalar parameters. The formalism is applied to calibrate the center element of the Parkes Multibeam receiver using observations of the millisecond pulsar, PSR J0437-4715, and the radio galaxy, 3C 218 (Hydra A).Comment: 8 pages, 4 figures, to be published in ApJ

An essential requirement for β1 integrin in the assembly of extracellular matrix proteins within the vascular wall

Abstractβ1 integrin has been shown to contribute to vascular smooth muscle cell differentiation, adhesion and mechanosensation in vitro. Here we showed that deletion of β1 integrin at the onset of smooth muscle differentiation resulted in interrupted aortic arch, aneurysms and failure to assemble extracellular matrix proteins. These defects result in lethality prior to birth. Our data indicates that β1 integrin is not required for the acquisition, but it is essential for the maintenance of the smooth muscle cell phenotype, as levels of critical smooth muscle proteins are gradually reduced in mutant mice. Furthermore, while deposition of extracellular matrix was not affected, its structure was disrupted. Interestingly, defects in extracellular matrix and vascular wall assembly, were restricted to the aortic arch and its branches, compromising the brachiocephalic and carotid arteries and to the exclusion of the descending aorta. Additional analysis of β1 integrin in the pharyngeal arch smooth muscle progenitors was performed using wnt1Cre. Neural crest cells deleted for β1 integrin were able to migrate to the pharyngeal arches and associate with endothelial lined arteries; but exhibited vascular remodeling defects and early lethality. This work demonstrates that β1 integrin is dispensable for migration and initiation of the smooth muscle differentiation program, however, it is essential for remodeling of the pharyngeal arch arteries and for the assembly of the vessel wall of their derivatives. It further establishes a critical role of β1 integrin in the protection against aneurysms that is particularly confined to the ascending aorta and its branches

Full Stokes polarimetric observations with a single-dish radio-telescope

The study of the linear and circular polarization in AGN allows one to gain detailed information about the properties of the magnetic fields in these objects. However, especially the observation of circular polarization (CP) with single-dish radio-telescopes is usually difficult because of the weak signals to be expected. Normally CP is derived as the (small) difference of two large numbers (LHC and RHC); hence an accurate calibration is absolutely necessary. Our aim is to improve the calibration accuracy to include the Stokes parameter V in the common single-dish polarimetric measurements, allowing a full Stokes study of the source under examination. A detailed study, up to the 2nd order, of the Mueller matrix elements in terms of cross-talk components allows us to reach the accuracy necessary to study circular polarization. The new calibration method has been applied to data taken at the 100-m Effelsberg radio-telescope during regular test observations of extragalactic sources at 2.8, 3.6, 6 and 11 cm. The D-terms in phase and amplitude appear very stable with time and the few known values of circular polarization have been confirmed. It is shown that, whenever a classical receiver and a multiplying polarimeter are available, the proposed calibration scheme allows one to include Stokes V in standard single-dish polarimetric observations as difference of two native circular outputs.Comment: 10 pages, to be published in A&

Mesenchymal Stromal Cell secretome is affected by tissue source and donor age.

Variation in Mesenchymal Stromal Cell (MSC) function depending on their origin is problematic, as it may confound clinical outcomes of MSC therapy. Current evidence suggests that the therapeutic benefits of MSCs are attributed to secretion of biologically active factors (secretome). However, the effect of donor characteristics on the MSC secretome remains largely unknown. Here, we examined the influence of donor age, sex and tissue source, on the protein profile of the equine MSC secretome. We used dynamic metabolic labelling with stable isotopes combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify secreted proteins in MSC conditioned media (CM). Seventy proteins were classified as classically-secreted based on the rate of label incorporation into newly synthesised proteins released into the extracellular space. Next, we analysed CM of bone marrow- (n = 14) and adipose-derived MSCs (n = 16) with label-free LC-MS/MS. Clustering analysis of 314 proteins detected across all samples identified tissue source as the main factor driving variability in MSC CM proteomes. Linear modelling applied to the subset of 70 secreted proteins identified tissue-related difference in the abundance of 23 proteins. There was an age-related decrease in the abundance of CTHRC1 and LOX, further validated with orthogonal techniques. Due to the lack of flow cytometry characterisation of MSC surface markers, the analysis could not account for the potential effect of cell population heterogeneity. This study provides evidence that tissue source and donor age contribute to differences in the protein composition of MSC secretomes which may influence the effects of MSC therapy