Effects of Propolis on Selected Blood Indicators and Antioxidant Enzyme Activities in Broilers under Heat Stress

In this study, we investigated the antioxidant activity of ethanol extracts of propolis (EEP) and vitamin C on biochemical indicators and antioxidant enzyme activities of broilers exposed to heat stress (at 34 °C). The experimental groups were as follows: group I (positive control) and group II (control) were fed a basal diet, group III (vitamin C) was fed a basal diet supplemented with 250 mg vitamin C as ascorbic acid/kg, group IV (EEP-0.5) was fed a basal diet supplemented with 0.5 g EEP/kg, group V (EEP-1) was fed a basal diet supplemented with 1 g EEP/kg, group VI (EEP-3) was fed a basal diet supplemented with 3 g EEP/kg. Plasma superoxide dismutase levels of positive control, control, vitamin C, EEP-0.5, EEP-1 and EEP-3 groups were found as 0.34, 1.23, 0.50, 0.90, 0.30 and 0.41 μkat/ml, respectively (p p p < 0.05), whereas those of muscle and heart were similar in all groups. The results of the present study suggest that EEP and specially EEP at the supplemented dose of 3 mg/kg diet might be considered to prevent oxidative stress in the broilers exposed to heat stress

Comparison of local rosmarinic acid and topical dexpanthenol applications on wound healing in a rat experimental wound model

Background: The aim of the study was to compare the effects of rosmarinic acid and dexpanthenol in a rat experimental wound model.Materials and methods: Twenty-four Wistar albino rats weighing 200–250 g were randomly divided into three groups. After 2-cm full-thickness skin defects were created, the wounds were washed with sterile 0.9% NaCl solution. After washing, the control group was left untreated, the second group received 5% dexpanthenol cream, and the third group received 10% rosmarinic acid cream. Before excision, the skin was evaluated macroscopically by measuring the reduction in wound size; after excision, histological examination (epithelisation, inflammation, fibrosis, granulation) was performed.Results: Macroscopic comparison of the wound sizes showed that group 3 showed a statistically significant difference in wound size reduction compared to the other two groups. Histopathological examination showed that there was no statistically significant difference between the groups. We found that the rosmarinic acid group had greater wound size reduction than the other two groups. However, epithelialisation was detected in fewer cases.Conclusions: We believe that rosmarinic acid can be used as a topical cream for wound healing, as it leads to significant reduction in wound size, resulting in fewer scars

The infant mortality rates and its causes in Hatay province during the years 2009 and 2010

Amaç: Gelişmekte olan ülkelerde sağlık sisteminin ve Ana-Çocuk Sağlığı hizmetlerinin kalitesini gösteren çağdaş parametrelerden en önemlisi “Bebek Ölüm Hızı”dır. Bu bağlamda bebek ölüm hızının değerlendirilmesi ve aşağıya çekilmesi toplum için çok önemlidir. Ülkemize bakıldığında bebek ölüm hızında son yıllarda ciddi iyileşme göze çarpmakla beraber hala il ve bölgeler bazında farklılıklar söz konusudur. Hatay ili de değişik sosyoekonomik yapısıyla bu farklılığı sunan bölgelerden birisi olup bu çalışmada ilimiz verilerini tartışmayı amaçladık. Gereç ve Yöntem: Hatay İl Sağlık Müdürlüğü Bebek Ölümlerini Takip ve Önleme Komisyonu tarafından saptanan Ocak 2009 ile Aralık 2010 tarihleri arasındaki sağlık ocağı ve hastane verileri incelendi. Bunun sonucunda bebek ölüm hızı, prematüre doğum hızı, diare, pnömoni, menenjit, sepsis, konjenital anomaliler ve bunlara etki eden maternal ve çevresel faktörler değerlendirildi. Bulgular: İlimizde 2009 yılında 29006 ve 2010 yılında ise 28352 canlı doğum gerçekleşmiş olup, 2009 yılında 617 (‰15.1) ve 2010 yılında 585 (‰13.4) bebek ölümü gerçekleşti. En sık karşılaşılan ölüm nedeni prematürite (n;185) iken bunu konjenital anomaliler, konjenital kalp hastalıkları, aspirasyon sendromu, respiratuar distres sendromu, sepsis ve alt solunum yolu enfeksiyonları izledi. Sonuç: İlimizde Bebek Ölüm Hızında anlamlı azalma olmuş, ülke ortalamasının altına inmiştir. Ölümlerin en önemli kısmını prematüre doğumlar ve konjenital anomaliler oluşturmaktadır.Aim: The infant mortality rate is a very important indicator of a mother&amp;#8217;s and child&amp;#8217;s health and health management in developing countries. For this reason, evaluation of the infant mortality rate and precautions for the reduction of it are particularly important. In our country, there is a substantial improvement in the infant mortality rates; however there still remains a disparity between different provinces and regions. In this study we aimed to examine the infant mortality rates and its causes in the province of Hatay, where there is a heterogeneous socioeconomically structure. Materials and Methods: The board established within the Directorate of Health of Hatay reviewed retrospectively the clinical charts and hospital records of babies born between January 2009 and December 2010. As a result, infant mortality and premature birth rates, diarrhea, pneumonia, meningitis, sepsis, congenital anomalies, and contributing maternal and environmental factors were evaluated. Results: In the province, there were 29,006 live births in 2009 and 28,352 in 2010. Of these, 617 (15.1&amp;#8240;) infant deaths occurred in 2009 and 585 (13.4&amp;#8240;) in 2010. Most of the deaths were related to prematurity (n=185), which was followed by congenital anomalies, especially congenital heart defects, aspiration syndrome, respiratory distress syndrome, sepsis and lower respiratory tract infections. Conclusion: In the last two years, the infantile mortality rate decreased in Hatay province, and also declined below the infant mortality rate for the nation. Premature births and congenital abnormalities comprise most of the causes of the deaths

Role of p38 MAPK, Akt and NFκB in renoprotective effects of nebivolol on renal ischemia-reperfusion injury in rats

Renal ischemia-reperfusion (I-R) injury is a complex pathophysiologic condition characterized by oxidative stress, inflammation and apoptosis. We investigated the potential renoprotective effect of nebivolol, a β1 adrenergic receptor blocker, against renal I-R injury. We focused on the role of nebivolol in activating p38 mitogen-activated protein kinase (MAPK) signaling, Akt (protein kinase B) and nuclear factor-κB (NFκB) transcription factors, which contribute to oxidative stress, inflammation and apoptosis during renal I-R. We divided 20 adult male Wistar albino rats into three experimental groups. Group 1 was a sham control in which only laparotomy was performed. Group 2 was the I-R group in which both kidneys were made ischemic for 45 min, then reperfused for 24 h. Group 3 was the I-R + nebivolol group in which 10 mg/kg nebivolol was administrated by gavage for 7 days before I-R. We measured Inflammation, oxidative stress and active caspase-3 as well as activation of p38 MAPK, Akt (protein kinase B) and NFκB transcription factor. Nebivolol significantly reduced oxidative stress and increased superoxide dismutase levels during renal I-R. We found that nebivolol significantly decreased interstitial inflammation, and TNF-α and interleukin-1β mRNA expression. Nebivolol significantly reduced active caspase-3 and kidney injury molecule-1 (KIM-1) expressions. Nebivolol also significantly decreased activation of p38 MAPK signaling and NFκB, and induced Akt activation during renal I-R. Our findings suggest that nebivolol may be useful for management of renal I-R injury. © 2023 The Biological Stain Commission

Cutaneous analgesia before transradial access for coronary intervention to prevent radial artery spasm

PMID = 2882535

Whole exome sequencing of consanguineous families of clinically diagnosed with neurodevelopmental disorders

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The Mir-644A/Ctbp1/P53 Axis Suppresses Drug Resistance By Simultaneous Inhibition Of Cell Survival And Epithelial-Mesenchymal Transition In Breast Cancer

Tumor cells develop drug resistance which leads to recurrence and distant metastasis. MicroRNAs are key regulators of tumor pathogenesis; however, little is known whether they can sensitize cells and block metastasis simultaneously. Here, we report miR-644a as a novel inhibitor of both cell survival and EMT whereby acting as pleiotropic therapy-sensitizer in breast cancer. We showed that both miR-644a expression and its gene signature are associated with tumor progression and distant metastasis-free survival. Mechanistically, miR-644a directly targets the transcriptional co-repressor C-Terminal Binding Protein 1 (CTBP1) whose knock-outs by the CRISPR-Cas9 system inhibit tumor growth, metastasis, and drug resistance, mimicking the phenotypes induced by miR-644a. Furthermore, downregulation of CTBP1 by miR-644a upregulates wild type- or mutant-p53 which acts as a ‘molecular switch’ between G1-arrest and apoptosis by inducing cyclin-dependent kinase inhibitor 1 (p21, CDKN1A, CIP1) or pro-apoptotic phorbol-12-myristate-13-acetate-induced protein 1 (Noxa, PMAIP1), respectively. Interestingly, an increase in mutant-p53 by either overexpression of miR-644a or downregulation of CTBP1 was enough to shift this balance in favor of apoptosis through upregulation of Noxa. Notably, p53-mutant patients, but not p53-wild type ones, with high CTBP1 have a shorter survival suggesting that CTBP1 could be a potential prognostic factor for breast cancer patients with p53 mutations. Overall, re-activation of the miR-644a/CTBP1/p53 axis may represent a new strategy for overcoming both therapy resistance and metastasis.PubMedWoSScopu