Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.

Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1-3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome

Analytical Method Development and Validation for the Simultaneous Estimation of Azelnidipine and Telmisartan by RP-HPLC in Bulk and Tablet Dosage Forms

An accurate, precise and reproducible RP-HPLC method was developed for the simultaneous quantitative determination of Azelnidipine and Telmisartan in tablet dosage forms. Agilent (S.K) Gradient System UV Detector and C18 column with 250mm x 4.6mm i.d and 5μm particle size Acetonitrile: ph Buffer (80:20v/v) pH 3 was used as the mobile phase for the method. The detection wavelength was 237nm and flow rate was 0.9 ml/min. In the developed method, the retention time of Azelnidipine and Telmisartan were found to be 4.77 min and 11.97min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. Hence the method was found to be simple, accurate, precise, economic and reproducible. The developed method was validated according to the ICH guidelines. In this methods linearity, precision, range, robustness were observed. The method was found to be simple, accurate, precise, economic and reproducible. So the proposed methods can be used for the routine quality control analysis of AZN and TMZ in bulk drug as well as in formulations.</jats:p

Natural dye yielding plants

228-234Every herb can be used to make dye. Herbal dyes being natural tend to be softer and their range of tones is very pleasant. At present total market of herbal dyes is to the tune of US $ 1 billion and is growing tremendously at the rate of 12%per annum. Per capita consumption of dyes is 400g to 15 kg in developed and underdeveloped countries for their utility in paints, inks, textiles, polymers, etc. India is a major exporter of herbal dyes mostly due to ban on production of some of the synthetic dyes and intermediates in the developed countries due to pollution problem. Nature has gifted us more than 500 colour yielding plants. The present paper is an aid to a collective enquiry into the Indian dye yielding plants, their parts and chemical constituents