Thermal Assessment of Convective Heat Transfer in Air- Cooled PEMFC Stacks: An Experimental Study

AbstractThis study presents an experimental stack-level thermal and hydrodynamic assessment of a model air-cooled PEM fuel cell. To mimic the heat generation inside the MEA, rubber heater films are used. Pressure drop along the stack channels and temperature distribution on the bipolar plate surface are measured for the channel Reynolds number range of 200-500 and the stack input power range of 100-250W. Tests are performed with and without gas diffusion layers (GDL) to investigate the effect of GDL and its surface characteristics on the pressure drop and heat transfer. Our results indicate that, with the existing length of bipolar plates, a major part of plate minichannel is filled with the developing region. This leads to a higher heat transfer rates, thus more uniform stack temperature can be obtained with the penalty of higher fan power. The minimum measured temperature difference is about 10°C and the values become more pronounced when the Reynolds number decreases. The existence of the GDL is observed to have negligible effect on the pressure drop

Determination of Settling Tanks Performance Using an Eulerian- Lagrangian Method

Circulation regions always exist in settling tanks. These regions reduce the tank’s performance and decrease its effective volume. The recirculation zones would result in short-circuiting and high flow mixing problems. The inlet position would also affect the size and location of the recirculation region. Using a proper baffle configuration could substantially increase the performance of the settling tanks. A common procedure for the comparison of the performances of different tanks has been using the Flow Through Curves (FTC) method. FTC, however, neglects tendencies for particles sedimentation. In this work, a new method for evaluation of the settling tanks performance is presented. The new method which is referred to as the particle Tracking Method (PTM) is based on an Eulerian-Lagrangian approach. In this paper, by using FTC and PTM the effects of the inlet position and the baffle configuration on the hydraulic performance of the primary settling tanks were studied and results were compared. Then, shortcomings of the FTC approach were stated. The optimal positioning of the baffles was also determined though a series of computer simulations

In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing

Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and in vivo results show that the in situ GelMA crosslinking induces a strong scaffold adhesion and enables printing on curved surfaces of wet tissues, without the need for any sutures. The scaffold is further shown to offer a sustained release of VEGF, enhancing the migration of endothelial cells in vitro. Histological analyses demonstrate that the administration of the VEGF-eluting GelMA scaffolds that remain adherent to the wound bed significantly improves the quality of healing in porcine wounds. The introduced in vivo printing strategy for wound healing applications is translational and convenient to use in any place, such as an operating room, and does not require expensive bioprinters or imaging modalities

A textile platform using mechanically reinforced hydrogel fibres towards engineering tendon niche

INTRODUCTION: Tendon injuries can result from tendon overuse or trauma, resulting in substantial pain and disability. Given that natural or surgical repair of tendons lead to a poor outcome in terms of mechanical properties and functionality, there is a great need for tissue engineering strategies. Textile platforms enable the generation of biomimetic constructs [1]. Therefore, the main goal of this study is the development of cell-laden hybrid hydrogel fibers reinforced with a mechanically robust core fiber and their assembly into braided constructs towards replicating tendon mechanical properties and architecture. METHODS: To fabricate mechanically reinforced hydrogel fibres, a commercially available suture was coated using a cell-hydrogel mixture of methacryloyl gelatine (GelMA) and alginate. Composite fibres (CFs) were obtained by ionic crosslinking of alginate followed by photocrosslinking of GelMA. CFs were assembled using braiding technique and the mechanical properties of single fibres and braided constructs were evaluated. Different cells were encapsulated in the hydrogel layer, including MC-3T3, mesenchymal stem cells (MSCs) and human tendon-derived cells (TDCs). Cell viability and metabolic activity were evaluated by LIVE/DEAD staining and presto blue assay of metabolic activity. The expression of tendon-related markers and matrix deposition were also investigated. RESULTS: CFs were fabricated with a GelMA:alginate hydrogel layer and using multifilament twisted cotton or biodegradable suturing threads. The biocompatibility of this system was evaluated on encapsulated cells (Fig.1a). Cells (MC-3T3, MSCs and TDCs) were homogeneously distributed along the hydrogel layer, being viable up to 14 days in culture. In addition, TDCs were spreading inside the hydrogel after less than 48 h. Moreover, to further improve the mechanical properties of CFs, braided constructs were generated (Fig. 1b). Braiding CFs together enhanced their tensile strength and the process did not affect the viability of encapsulated cells.DISCUSSION & CONCLUSIONS: CFs were generated with a load bearing core and a hydrogel layer towards mimicking both mechanical properties and the matrix-rich microenvironment of tendon tissue. Accordingly, cell behaviour can be further modulated by modifying the hydrogel composition or, ultimately, through the addition of bioactive cues. Finally, braiding CFs together allows tuning the mechanical properties of developed constructs to match those of native tendon tissues.Fundação para a Ciência e a Tecnologia in the framework of FCT-POPH-FSE, the PhD grant SFRH/BD/96593/2013 of R.C-

The Effect of Poly (Glycerol Sebacate) Incorporation within Hybrid Chitin–Lignin Sol–Gel Nanofibrous Scaffolds

Chitin and lignin primarily accumulate as bio-waste resulting from byproducts of crustacean crusts and plant biomass. Recently, their use has been proposed for diverse and unique bioengineering applications, amongst others. However, their weak mechanical properties need to be improved in order to facilitate their industrial utilization. In this paper, we fabricated hybrid fibers composed of a chitin–lignin (CL)-based sol–gel mixture and elastomeric poly (glycerol sebacate) (PGS) using a standard electrospinning approach. Obtained results showed that PGS could be coherently blended with the sol–gel mixture to form a nanofibrous scaffold exhibiting remarkable mechanical performance and improved antibacterial and antifungal activity. The developed hybrid fibers showed promising potential in advanced biomedical applications such as wound care products. Ultimately, recycling these sustainable biopolymers and other bio-wastes alike could propel a “greener” economy

Neuroprotective and anti-inflammatory effects of Rhus coriaria extract in a mouse model of ischemic optic neuropathy

Modulating oxidative stresses and inflammation can potentially prevent or alleviate the pathological conditions of diseases associated with the nervous system, including ischemic optic neuropathy. In this study we evaluated the anti-neuroinflammatory and neuroprotective activities of Rhus coriaria (R. coriaria) extract in vivo. The half maximal inhibitory concentration (IC50) for DPPH, ABTS and \u3b2-carotene were 6.79 \ub1 0.009 \u3bcg/mL, 10.94 \ub1 0.09 \u3bcg/mL, and 6.25 \ub1 0.06 \u3bcg/mL, respectively. Retinal ischemia was induced by optic nerve crush injury in albino Balb/c mice. The anti-inflammatory activity of ethanolic extract of R. coriaria (ERC) and linoleic acid (LA) on ocular ischemia was monitored using Fluorescence Molecular Tomography (FMT). Following optic nerve crush injury, the mice treated with 400 mg/kg of ERC and LA exhibited an 84.87% and 86.71% reduction of fluorescent signal (cathepsin activity) respectively. The results of this study provide strong scientific evidence for the neuroprotective activity of the ERC, identifying LA as one of the main components responsible for the effect. ERC may be useful and worthy of further development for its adjunctive utilization in the treatment of optic neuropathy

Theoretical study of Oldroyd-b visco-elastic fluid flow through curved pipes with slip effects in polymer flow processing

The characteristics of the flow field of both viscous and viscoelastic fluids passing through a curved pipe with a Navier slip boundary condition have been investigated analytically in the present study. The Oldroyd-B constitutive equation is employed to simulate realistic transport of dilute polymeric solutions in curved channels. In order to linearize the momentum and constitutive equations, a perturbation method is used in which the ratio of radius of cross section to the radius of channel curvature is employed as the perturbation parameter. The intensity of secondary and main flows is mainly affected by the hoop stress and it is demonstrated in the present study that both the Weissenberg number (the ratio of elastic force to viscous force) and slip coefficient play major roles in determining the strengths of both flows. It is also shown that as a result of an increment in slip coefficient, the position of maximum velocity markedly migrates away from the pipe center towards the outer side of curvature. Furthermore, results corresponding to Navier slip scenarios exhibit non-uniform distributions in both the main and lateral components of velocity near the wall which can notably vary from the inner side of curvature to the outer side. The present solution is also important in polymeric flow processing systems because of experimental evidence indicating that the no-slip condition can fail for these flows, which is of relevance to chemical engineers

In-plane gas permeability of proton exchange membrane fuel cell gas diffusion layers. J. Power Sour, 196: 3559–3564. Tafaoli-Masoule et al

a b s t r a c t A new analytical approach is proposed for evaluating the in-plane permeability of gas diffusion layers (GDLs) of proton exchange membrane fuel cells. In this approach, the microstructure of carbon papers is modeled as a combination of equally-sized, equally-spaced fibers parallel and perpendicular to the flow direction. The permeability of the carbon paper is then estimated by a blend of the permeability of the two groups. Several blending techniques are investigated to find an optimum blend through comparisons with experimental data for GDLs. The proposed model captures the trends of experimental data over the entire range of GDL porosity. In addition, a compact relationship is reported that predicts the inplane permeability of GDL as a function of porosity and the fiber diameter. A blending technique is also successfully adopted to report a closed-form relationship for in-plane permeability of three-directional fibrous materials

ESFuelCell2011-54257 Through-Plane Gas Permeability of Proton Exchange Membrane Fuel Cell Gas Diffusion Layers

Abstract Effects of mechanical compression and PTFE content on the through-plane gas permeability of gas diffusion layers (GDLs) of PEM fuel cells are investigated both experimentally and theoretically. A new test bed is designed and built which allows pressure drop and air flow rate measurement for various fibrous samples. The measured values are used to calculate the through-plane permeability. Various GDLs are obtained and tested over a wide range of PTFE content and compression ratio. The experimental data shows a reverse relationship between the through-plane permeability and both PTFE content and mechanical compression. An existing model for through-plane permeability of planar structures is revisited to develop a model that accommodates effects of PTFE content and GDL compression. The proposed model captures the trends of the experimental data for through-plane permeability, measured in the present study or reported by others