Convergent evolution of prehistoric technologies: the entropy and diversity of limited solutions

Linking the likelihood of convergent evolution to the technologies’ complexity, this paper identifies the scales of technological diffusion and convergence, i.e., the evolving of structures that are similar, but not related to a common “ancestor.” Our study provides quantitative measures for understanding complexity and connectivity in technologies. The utility of our approach is exemplified through the case study of Cucuteni-Tripolye pottery kilns in Chalcolithic Southeastern Europe. The analysis shows that technological evolution has to be scaled to the “technologically important” (in quantitative terms) component parts, whose introduction shapes a ground for extinction and self-evolvement caused by the cascade effects along technological design structure. Similar technological solutions to the technological design structure engender the spread of similar devices in various locations. Surprisingly, such a broad distribution may be the result of relatively low internal diversity, rather than arising from higher efficiency. This gives some reasons for the underestimation of convergence as a mechanism for evolution of technology in current prehistoric archaeology

DLS setup for in-situ measurements of photo-induced size changes of microgel-based hybrid particles

Photoinduced size changes in microgel particles loaded with gold nanoparticles (AuNPs) were investigated with an extended multiangle dynamic light scattering (DLS) setup. The DLS setup was equipped with a conventional laser (λ = 633 nm) to determine the microgel particle size. Additionally, a laser (λ = 532 nm) is installed to study the photoresponsive behavior of the AuNP–microgel hybrids. The wavelength of 532 nm is close to the absorption maximum of the plasmon resonance of the AuNPs used in the present study (i.e. spherical AuNPs with a diameter of 14 nm). The extended DLS setup enables us to follow in situ the change in microgel size during irradiation. The light stimulus is directly correlated with the size changes of the hybrid particles and the photothermal effect depends on the intensity of the excitation laser. The increase in excitation laser intensity results in a size reduction of hybrid particles because of the ability of AuNPs to partially transform the absorbed photon energy into heat which is emitted into the surrounding microgel network

Genetic determinants of co-accessible chromatin regions in activated T cells across humans.

Over 90% of genetic variants associated with complex human traits map to non-coding regions, but little is understood about how they modulate gene regulation in health and disease. One possible mechanism is that genetic variants affect the activity of one or more cis-regulatory elements leading to gene expression variation in specific cell types. To identify such cases, we analyzed ATAC-seq and RNA-seq profiles from stimulated primary CD4+ T cells in up to 105 healthy donors. We found that regions of accessible chromatin (ATAC-peaks) are co-accessible at kilobase and megabase resolution, consistent with the three-dimensional chromatin organization measured by in situ Hi-C in T cells. Fifteen percent of genetic variants located within ATAC-peaks affected the accessibility of the corresponding peak (local-ATAC-QTLs). Local-ATAC-QTLs have the largest effects on co-accessible peaks, are associated with gene expression and are enriched for autoimmune disease variants. Our results provide insights into how natural genetic variants modulate cis-regulatory elements, in isolation or in concert, to influence gene expression

Unlike for Human Monocytes after LPS Activation, Release of TNF-α by THP-1 Cells Is Produced by a TACE Catalytically Different from Constitutive TACE

Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine today identified as a key mediator of several chronic inflammatory diseases. TNF-α, initially synthesized as a membrane-anchored precursor (pro-TNF-α), is processed by proteolytic cleavage to generate the secreted mature form. TNF-α converting enzyme (TACE) is currently the first and single protease described as responsible for the inducible release of soluble TNF-α.Here, we demonstrated the presence on THP-1 cells as on human monocytes of a constitutive proteolytical activity able to cleave pro-TNF-α. Revelation of the cell surface TACE protein expression confirmed that the observed catalytic activity is due to TACE. However, further studies using effective and innovative TNF-α inhibitors, as well as a highly selective TACE inhibitor, support the presence of a catalytically different sheddase activity on LPS activated THP-1 cells. It appears that this catalytically different TACE protease activity might have a significant contribution to TNF-α release in LPS activated THP-1 cells, by contrast to human monocytes where the TACE activity remains catalytically unchanged even after LPS activation.On the surface of LPS activated THP-1 cells we identified a releasing TNF-α activity, catalytically different from the sheddase activity observed on human monocytes from healthy donors. This catalytically-modified TACE activity is different from the constitutive shedding activity and appears only upon stimulation by LPS

Decoding human fetal liver haematopoiesis.

Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using single-cell transcriptome profiling of approximately 140,000 liver and 74,000 skin, kidney and yolk sac cells, we identify the repertoire of human blood and immune cells during development. We infer differentiation trajectories from HSC/MPPs and evaluate the influence of the tissue microenvironment on blood and immune cell development. We reveal physiological erythropoiesis in fetal skin and the presence of mast cells, natural killer and innate lymphoid cell precursors in the yolk sac. We demonstrate a shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs, which we functionally validate. Our integrated map of fetal liver haematopoiesis provides a blueprint for the study of paediatric blood and immune disorders, and a reference for harnessing the therapeutic potential of HSC/MPPs.We acknowledge funding from the Wellcome Human Cell Atlas Strategic Science Support (WT211276/Z/18/Z); M.H. is funded by Wellcome (WT107931/Z/15/Z), The Lister Institute for Preventive Medicine and NIHR and Newcastle-Biomedical Research Centre; S.A.T. is funded by Wellcome (WT206194), ERC Consolidator and EU MRG-Grammar awards and; S.B. is funded by Wellcome (WT110104/Z/15/Z) and St. Baldrick’s Foundation; E.L. is funded by a Wellcome Sir Henry Dale and Royal Society Fellowships, European Haematology Association, Wellcome and MRC to the Wellcome-MRC Cambridge Stem Cell Institute and BBSRC

Noise-Driven Stem Cell and Progenitor Population Dynamics

BACKGROUND: The balance between maintenance of the stem cell state and terminal differentiation is influenced by the cellular environment. The switching between these states has long been understood as a transition between attractor states of a molecular network. Herein, stochastic fluctuations are either suppressed or can trigger the transition, but they do not actually determine the attractor states. METHODOLOGY/PRINCIPAL FINDINGS: We present a novel mathematical concept in which stem cell and progenitor population dynamics are described as a probabilistic process that arises from cell proliferation and small fluctuations in the state of differentiation. These state fluctuations reflect random transitions between different activation patterns of the underlying regulatory network. Importantly, the associated noise amplitudes are state-dependent and set by the environment. Their variability determines the attractor states, and thus actually governs population dynamics. This model quantitatively reproduces the observed dynamics of differentiation and dedifferentiation in promyelocytic precursor cells. CONCLUSIONS/SIGNIFICANCE: Consequently, state-specific noise modulation by external signals can be instrumental in controlling stem cell and progenitor population dynamics. We propose follow-up experiments for quantifying the imprinting influence of the environment on cellular noise regulation.Engineering and Applied SciencesOther Research Uni

Analysis of the chosen attributes of different types of luxmeters

Podstawowym i najczęściej stosowanym miernikiem w fotometrii jest luksomierz. Aby mierzone natężenie oświetlenia odpowiadało wartości odniesienia, miernik ten musi spełniać szereg wymagań, do których zalicza się m.in. korekcję widmową oraz przestrzenną głowicy foto-metrycznej luksomierza. W ostatnich latach na rynku można spotkać coraz więcej fotometrów, które z uwagi na niską cenę cieszą się dużym zainteresowaniem. Bardzo często ich koszt jednostkowy jest niższy od ceny wzorcowania. W artykule, na podstawie przeprowadzonych pomiarów laboratoryjnych, przeanalizowano wybrane właściwości metrologiczne powszechnie używanych typów luksomierzy podczas weryfikacji parametrów oświetlenia we wnętrzach. Pomiarom poddano zarówno mierniki o wysokiej, jak i o niższej dokładności. Wobec faktu, że do oświetlania wnętrz stosowane są źródła światła o różnych rozkładach widmowych emitowanego promieniowania, zarejestrowano wskazania poszczególnych luksomierzy przy oświetleniu ich powierzchni światłoczułych lampami wyładowczymi oraz źródłami LED. Uzyskane wartości odniesiono do pomiarów otrzymanych przy użyciu lampy żarowej o temperaturze barwowej Tc = 2856 K – źródle, którego promieniowanie wykorzystywane jest przy wzorcowaniu fotometrów. Na podstawie przeprowadzonych badań wyznaczono błędy względne oraz błędy kosinusowe luksomierzy, a także współczynniki (wskaźniki) korekcji barwy. Pomiary uzupełniono rejestracją rozkładów widmowych źródeł światła wykorzystanych podczas pomiarów eksperymentalnych.An iluminance meter is the basic and most frequently used measuring instrument in photometry. This instrument must meet a number of requirements e.g. a spectral and space correction of the photoelement in order to have the illuminance corresponding to the actual value. In the recent years, more and more photometers can be found on the market, which have generated a lot of attention because of their low price. Their individual cost is very often lower than the calibration price. On the grounds of the performed laboratory measurements, the chosen metrological attributes of commonly used luxmeters during the indoor verification of lighting parameters were analysed in the article. Instruments of high as well as low accuracy were tested. During the indoor lighting the light sources of different spectral concentration are used. Concerning this fact the individual luxmeters read-outs with light-sensitive surfaces lighted by the discharge lamp as well as LED sources were registered. Obtained results were compared with the values received during measurements carried out with the use of an electric lamp of the colour temperature Tc = 2856 K, i.e. a source, which radiation is used during the calibration of photometers. The relative and cosine errors as well as the colour correction coefficients (factors) were set down based on the carried out measurements. They were complemented with the registration of the spectral concentration locus of the light sources used during the experimental measurements

Product Quality Assessment – Measurement and Analysis of Surface Topography

This paper concerns the issues of measurement techniques, analysis and assessment of the machined surface geometric structure. The aim of this work was to show the application of surface analysis in diagnosing the causes of discrepancies occurring in the manufacturing process, which may result from ill-matched (poorly fitting) process parameters. An appropriate system of control and interpretation of results may allow early reaction to unfavorable trends (for example blunting of the tool) and prevention of undesirable defects. The subject of research was a waste basket used in the construction of retaining sewer systems. In this paper, the quality of the waste basket as well as its manufacturing process were analyzed and assessed. The research was carried out with the use of three measurement stands, i.e. optical microscopy (OM), scanning electron microscopy (SEM) and white light interferometer (WLI). The surface analysis proved to be important from the viewpoint of outlining the production process as well as improving the product quality. The software used for topographical analysis appeared to be significant for the success of the analysis, providing notable economic effects, namely the lack of defects