732 research outputs found
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Gentian violet for pyoderma gangrenosum: a retrospective chart review
Pyoderma gangrenosum is a rare autoinflammatory skin disease. Treatment is multifactorial, addressing inflammation, pain, underlying disease, if present, and the wound. Gentian violet has been used for hundreds of years in a variety of dermatologic conditions for its anti-inflammatory properties. This study aims to evaluate gentian violet in wound healing for pyoderma gangrenosum. We conducted a retrospective chart review of patients with pyoderma gangrenosum treated with gentian violet at the Wake Forest School of Medicine Department of Dermatology in the last 10 years. The primary outcome was clinical improvement. Of the 34 cases that met inclusion criteria, 70% improved with gentian violet, 24% had no documented change, 3% initially improved then worsened, and 3% had unclear results. Gentian violet is a safe and cheap treatment that may improve resolution of pyoderma gangrenosum lesions in addition to systemic therapy
Selenium Deficiency and Chronic Pancreatitis: Disease Mechanism and Potential for Therapy
Background: It has been suggested that antioxidant
deficiency may play a role in the pathogenesis of
chronic pancreatitis. The aim of this review was to
analyse the evidence for this relationship and to
consider the role of antioxidant supplementation in
the treatment of chronic pancreatitis
Immunostaining in Mohs Micrographic Surgery: A Review
With the advent of incorporating the immunoperoxidase staining technique into the processing of frozen tissue, the use of Mohs micrographic surgery (MMS) has been expanded to include several high-risk tumors such as lentigo maligna, malignant melanoma, and dermatofibrosarcoma protuberans.To thoroughly review the English medical literature pertaining to the use of immunohistochemical staining techniques on frozen sections during MMS and to summarize the basic relevant outcomes from the different relevant studies.Medline search was conducted, with the following words used in the search criteria: âMohs surgery,ââstaining,ââimmunostaining,â and âimmunoperoxidase.âGenerally, all immunostains showed advantage over the traditional hematoxylin and eosin approach. Studies of MART-1 in melanoma chemosurgery indicated that it is typically crisp and has less background staining than MEL-5 and better staining consistency than HMB-45. In cases of desmoplastic melanomas, S100 is the stain of choice.Immunostaining offers an advantage in MMS. Large, randomized, prospective studies comparing the different immunostains are still lacking in the literature. The authors have indicated no significant interest with commercial supporters.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/79305/1/j.1524-4725.2009.01432.x.pd
Analysis of mitotic phosphorylation of Borealin
BACKGROUND: The main role of the chromosomal passenger complex is to ensure that Aurora B kinase is properly localized and activated before and during mitosis. Borealin, a member of the chromosomal passenger complex, shows increased expression during G2/M phases and is involved in targeting the complex to the centromere and the spindle midzone, where it ensures proper chromosome segregation and cytokinesis. Borealin has a consensus CDK1 phosphorylation site, threonine 106 and can be phosphorylated by Aurora B Kinase at serine 165 in vitro. RESULTS: Here, we show that Borealin is phosphorylated during mitosis in human cells. Dephosphorylation of Borealin occurs as cells exit mitosis. The phosphorylated form of Borealin is found in an INCENP-containing complex in mitosis. INCENP-containing complexes from cells in S phase are enriched in the phosphorylated form suggesting that phosphorylation may encourage entry of Borealin into the chromosomal passenger complex. Although Aurora B Kinase is found in complexes that contain Borealin, it is not required for the mitotic phosphorylation of Borealin. Mutation of T106 or S165 of Borealin to alanine does not alter the electrophoretic mobility shift of Borealin. Experiments with cyclohexamide and the phosphatase inhibitor sodium fluoride suggest that Borealin is phosphorylated by a protein kinase that can be active in interphase and mitosis and that the phosphorylation may be regulated by a short-lived phosphatase that is active in interphase but not mitosis. CONCLUSION: Borealin is phosphorylated during mitosis. Neither residue S165, T106 nor phosphorylation of Borealin by Aurora B Kinase is required to generate the mitotic, shifted form of Borealin. Suppression of phosphorylation during interphase is ensured by a labile protein, possibly a cell cycle regulated phosphatase
Substructure in the Andromeda Galaxy Globular Cluster System
In the most prominent current scenario of galaxy formation, galaxies form
hierarchically through the merger of smaller systems. Such mergers could leave
behind dynamical signatures which may linger long after the event. In
particular, the globular cluster system (GCS) of a merging satellite galaxy may
remain as a distinct sub-population within the GCS of a massive galaxy. Using
the latest available globular cluster velocities and metallicities, we present
the results of a search for grouping in the GCS of our nearest large spiral
galaxy neighbor, M31. A modified friends-of-friends algorithm is used to
identify a number of possible merger remnants in projected position, radial
velocity and [Fe/H] parameter space. Numerical simulations are used to check
that such merger remnants are indeed plausible over the timescales of interest.
The identification of stellar streams associated with these groups is required
in order to confirm that they represent merger remnants.Comment: 19 pages, 3 figures, accepted for publication in the Ap
Raster-scan imaging with normal-incidence, midinfrared InAs/GaAs quantum dot infrared photodetectors
We demonstrate normal incidence infrared imaging with quantum dot infrared photodetectors using a raster-scan technique. The device heterostructure, containing multiple layers of InAs/GaAs self-organized quantum dots, were grown by molecular-beam epitaxy. Individual devices have been operated at temperatures as high as 150 K and, at 100 K, are characterized by λpeak = 3.72âÎŒm,λpeak=3.72ÎŒm, Jdark = 6Ă10â10âA/cm2Jdark=6Ă10â10A/cm2 for a bias of 0.1 V, and Dâ = 2.94Ă109âcmâHz1/2/WDâ=2.94Ă109cmHz1/2/W at a bias of 0.2 V. Raster-scan images of heated objects and infrared light sources were obtained with a small (13Ă13)(13Ă13) interconnected array of detectors (to increase the photocurrent) at 80 K. © 2002 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70691/2/APPLAB-80-18-3265-1.pd
ATR promotes cilia signalling: links to developmental impacts
Mutations in ATR (ataxia telangiectasia and RAD3-related) cause Seckel syndrome (ATR-SS), a microcephalic primordial dwarfism disorder. Hitherto, the clinical manifestation of ATR deficiency has been attributed to its canonical role in DNA damage response signalling following replication fork stalling/collapse. Here, we show that ATR regulates cilia-dependent signalling in a manner that can be uncoupled from its function during replication. ATR-depleted or patient-derived ATR-SS cells form cilia of slightly reduced length but are dramatically impaired in cilia-dependent signalling functions, including growth factor and Sonic hedgehog signalling. To better understand the developmental impact of ATR loss of function, we also used zebrafish as a model. Zebrafish embryos depleted of Atr resembled ATR-SS morphology, showed a modest but statistically significant reduction in cilia length and other morphological features indicative of cilia dysfunction. Additionally, they displayed defects in left-right asymmetry including ambiguous expression of southpaw, incorrectly looped hearts and randomized localization of internal organs including the pancreas, features typically conferred by cilia dysfunction. Our findings reveal a novel role for ATR in cilia signalling distinct from its canonical function during replication and strengthen emerging links between cilia function and development
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Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation
Centrosome separation in late G2/ early prophase requires precise spatial coordination that is determined by a balance of forces promoting and antagonizing separation. The major effector of centrosome separation is the kinesin Eg5. However, the identity and regulation of Eg5-antagonizing forces is less well characterized. By manipulating candidate components, we find that centrosome separation is reversible and that separated centrosomes congress toward a central position underneath the flat nucleus. This positioning mechanism requires microtubule polymerization, as well as actin polymerization. We identify perinuclear actin structures that form in late G2/early prophase and interact with microtubules emanating from the centrosomes. Disrupting these structures by breaking the interactions of the linker of nucleoskeleton and cytoskeleton (LINC) complex with perinuclear actin filaments abrogates this centrosome positioning mechanism and causes an increase in subsequent chromosome segregation errors. Our results demonstrate how geometrical cues from the cell nucleus coordinate the orientation of the emanating spindle poles before nuclear envelope breakdown
Beneath Illinois roads : from the surface to bedrock
"Surface deposits compiled from Hansel and Johnson (1996), Lineback (1979), and William and Frye (1970)." / Includes col. ill., glacial timetable, and ancillary map. / On verso: "Beneath Illinois roads: from the surface to bedrock," 1 cross section and 5 ancillary maps with text: Land cover -- Surface topography -- Surface deposits -- Bedrock surface -- Bedrock geology. Scale 1:760,320. 1 in. = approx. 12 mi.Ope
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