Flow cytometry application in the assessment of sperm DNA integrity of men with asthenozoospermia.

Sperm genomic integrity and ultrastructural features of ejaculated spermatozoa contributing to the assessment of gamete fertility potential in patients with asthenozoospermia are discussed. The proportion of TUNEL-positive cells was significantly higher in the semen of patients with low sperm motility (n=40; p4% of TUNEL-positive sperm cells. Low sperm motility seems to be accompanied by serious defects of gamete chromatin expressed as diminished sperm genomic integrity and abnormal DNA condensation and by defects of sperm midpiece. These abnormalities may reflect developmental failure during the spermatogenic remodeling process. The DNA fragmentation test may be considered as an additional assay for the evaluation of spermatozoa beside standard analysis and taken together with electron microscopy may help to determine the actual number of "healthy" spermatozoa thereby playing an important role during diagnosis and treatment of male infertility

Total hemoglobin mass, aerobic capacity, and hbb gene in polish road cyclists

The relationship between genes, amount of hemoglobin, and physical performance are still not clearly defined. The aim of this study was to examine the association between-551C/T and intron 2, +16 C/G polymorphisms in the beta hemoglobin (HBB) gene and total hemoglobin mass (tHbmass) and aerobic capacity in endurance athletes. Total hemoglobin mass and aerobic capacity indices, i. e.,VO2max, oxygen uptake at anaerobic threshold (VO2AT), maximal power output (Pmax), and power at anaerobic threshold (PAT) were determined in 89 young road cyclists, female (n = 39) and male (n = 50), who were genotyped for 2 polymorphisms in the HBB gene. The relative values of aerobic capacity indices differed significantly among intron 2, +16 C/G polymorphisms of the HBB gene only in female cyclists; athletes with GG genotype had significantly higher values of V O2max (p = 0.003), VO2AT (p = 0.007), PAT (p = 0.015), and Pmax (p = 0.004) than C carriers. No relationships were found between the C-carrier model (CC + CG vs. GG in the case of intron 2, +16 C/G and CC + CT vs. TT for -551 C/T polymorphisms of the HBB gene) and relative values of tHbmass. Our results demonstrated that the HBB gene could be related to aerobic capacity, but it seems that it does not result from an increase in the amount of hemoglobin in the blood

A Genetic Tuning to Improve the Performance of Fuzzy Rule-Based Classification Systems with Interval-Valued Fuzzy Sets: Degree of Ignorance and Lateral Position

Fuzzy Rule-Based Systems are appropriate tools to deal with classification problems due to their good properties. However, they can suffer a lack of system accuracy as a result of the uncertainty inherent in the definition of the membership functions and the limitation of the homogeneous distribution of the linguistic labels. The aim of the paper is to improve the performance of Fuzzy Rule-Based Classification Systems by means of the Theory of Interval-Valued Fuzzy Sets and a post-processing genetic tuning step. In order to build the Interval-Valued Fuzzy Sets we define a new function called weak ignorance for modeling the uncertainty associated with the definition of the membership functions. Next, we adapt the fuzzy partitions to the problem in an optimal way through a cooperative evolutionary tuning in which we handle both the degree of ignorance and the lateral position (based on the 2-tuples fuzzy linguistic representation) of the linguistic labels. The experimental study is carried out over a large collection of data-sets and it is supported by a statistical analysis. Our results show empirically that the use of our methodology outperforms the initial Fuzzy Rule-Based Classification System. The application of our cooperative tuning enhances the results provided by the use of the isolated tuning approaches and also improves the behavior of the genetic tuning based on the 3-tuples fuzzy linguistic representation.Spanish Government TIN2008-06681-C06-01 TIN2010-1505