Thresholds of terrestrial nutrient loading for the development of eutrophication episodes in a coastal embayment in the Aegean Sea

Thresholds of terrestrial nutrient loading (inorganic N and P) for the development of eutrophication episodes were estimated in an enclosed embayment, the gulf of Kalloni, in the Aegean, Eastern Mediterranean. Terrestrial loading was quantified by a watershed runoff model taking into account land use, geomorphology, sewerage, industrial and animal farming by-products. The eutrophication episodes were assessed by an existing scale for the Aegean coastal waters based on chl a, whereas the necessary nutrient concentrations (N and P) for the development of such episodes were defined using a probabilistic procedure. Finally, for the linking between nutrient loading arriving at the gulf and the resulting nutrient enrichment of the marine ecosystem, three loading factors were applied, developed by Vollenweider for lake and marine ecosystems. The first assumes no exchange between the embayment and the open sea, whereas the two others take into account water renewal time. Only the threshold for inorganic nitrogen estimated by the first factor was exceeded in the study area during February after a strong rainfall event coinciding with a eutrophication episode observed in the interior of the gulf, implying that the waters of the gulf are rather confined and the receiving body operates as a lake. The degree of confinement was further examined by studying the temperature, salinity, and density distributions inside the gulf and across the channel connecting the gulf to the open sea. It was found that the incoming freshwater from the watershed during winter results to the formation of a dilute surface layer of low salinity and density, clearly isolated from the open sea. The nutrients from the river inputs are diluted into this isolated water mass and the eutrophication threshold for nitrogen is exceeded. Although phosphorus loading was also high during winter, the corresponding limits were never exceeded. The proposed methodology sets a quantitative relationship between terrestrial nutrient loading and the development of eutrophication episodes in coastal embayments, assuming that information on the physical setting of the system is available. These cause-and-effect relationships can be invaluable tools for managers and decision makers in the framework of Integrated Coastal Zone Management

Limited Depressive and Anxiety Symptoms Late in Pregnancy Are Not Related to Neonatal Outcomes

Background: Prior studies have reported inconsistent findings regarding the link between antenatal depressive and anxiety symptomatology, with neonatal outcomes. Objectives: The aim of the present study was to assess the possible association of prenatal depressive and anxiety symptoms, in the third trimester of pregnancy, with perinatal outcomes (birth weight of the newborn, Apgar score and the newborn’s admission in neonatal intensive care unit) in a sample of pregnant women, in Greece. Patients and Methods: A total of 117 women from Athens, during the 32nd to 35th week of pregnancy, participated in the study. Demographic and obstetric history data, as well as neonatal outcomes, were recorded. Three self-administered psychometric scales (Beck depression inventory (BDI), Edinburg postnatal depression scale (EPDS) and beck anxiety inventory (BAI)) were used to evaluate in detail the prenatal depressive and anxiety symptoms. Descriptive statistics, Spearman’s Rho coefficients, Mann-Whitney U and Kruskal-Wallis testes were applied to analyze the data. Results: On the basis of BDI, 81.1% of the sample showed minimal, 15.4% mild, 2.6% moderate and 0.9% severe depressive symptoms, respectively. Furthermore, 80.3% of the participants, scored on EPDS below the cut-off point for a likely diagnosis of depression. According to BAI scale, 43.6% showed minimal, 42.7% women mild, 10.3% moderate and 3.4% severe anxiety symptoms. No statistically significant correlations were found between depressive and anxiety symptoms and neonatal outcomes (birth weight, Apgar score and admission in neonatal intensive care unit). Conclusions: Limited levels of prenatal depressive and anxiety symptoms do not seem to be associated with neonatal outcomes. In clinical practice, pregnant women, who suffer from low levels of prenatal depressive and anxiety symptoms, may be reassured, in respect of the adverse outcomes of these mood symptoms on the neonate

Linking shyness to social anxiety in children through the Clark and Wells cognitive model

This is the author accepted manuscript. The final version is available from Ellinika Grammata via the link in this record.Past research has begun to show that cognitive biases partially mediate the relation between shyness and social anxiety. In addition, it has been showed that the Clark and Wells (1995) cognitive model generalizes to youth. This study investigated the mediating role of the model in the link between shyness and social anxiety. Participants were 306 preadolescents, who completed measures of shyness, social anxiety, and cognitive variables implicated by the model (anticipatory processing, post-event processing, and social attitudes). The results confirmed that shyness, social anxiety and maladaptive cognitive processes were intercorrelated. Further, in a multiple mediator model, social attitudes, but not anticipatory or post-event processing, partially mediated the relation between shyness and social anxiety. Implications for school prevention interventions are briefly discussed

A new mathematical model for the interpretation of translational research evaluating six CTLA-4 polymorphisms in high-risk melanoma patients receiving adjuvant interferon

Adjuvant therapy of stage IIB/III melanoma with interferon reduces relapse and mortality by up to 33% but is accompanied by toxicity-related complications. Polymorphisms of the CTLA-4 gene associated with autoimmune diseases could help in identifying interferon treatment benefits. We previously genotyped 286 melanoma patients and 288 healthy (unrelated) individuals for six CTLA-4 polymorphisms (SNP). Previous analyses found no significant differences between the distributions of CTLA-4 polymorphisms in the melanoma population vs. controls, no significant difference in relapse free and overall survivals among patients and no correlation between autoimmunity and specific alleles. We report new analysis of these CTLA-4 genetic profiles, using Network Phenotyping Strategy (NPS). It is graph-theory based method, analyzing the SNP patterns. Application of NPS on CTLA-4 polymorphism captures allele relationship pattern for every patient into 6-partite mathematical graph P. Graphs P are combined into weighted 6-partite graph S, which subsequently decomposed into reference relationship profiles (RRP). Finally, every individual CTLA-4 genotype pattern is characterized by the graph distances of P from eight identified RRP's. RRP's are subgraphs of S, collecting equally frequent binary allele co-occurrences in all studied loci. If S topology represents the genetic "dominant model", the RRP's and their characteristic frequencies are identical to expectation-maximization derived haplotypes and maximal likelihood estimates of their frequencies. The graphrepresentation allows showing that patient CTLA-4 haplotypes are uniquely different from the controls by absence of specific SNP combinations. New function-related insight is derived when the 6-partite graph reflects allelic state of CTLA-4. We found that we can use differences between individual P and specific RRPs to identify patient subpopulations with clearly different polymorphic patterns relatively to controls as well as to identify patients with significantly different survival. © 2014 Pancoska et al

RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes

Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S. lycopersicum genome resulted in the discovery of two additional sesquiterpene synthases expressed in trichomes. The sesquiterpene synthases from S. lycopersicum and S. habrochaites have high levels of protein identity. Several of them appeared to encode for non-functional proteins. Functional recombinant proteins produced germacrenes, β-caryophyllene/α-humulene, viridiflorene and valencene from (E,E)-farnesyl diphosphate. However, the activities of these enzymes do not completely explain the differences in sesquiterpene production between the two tomato plants. RT-qPCR confirmed high levels of expression of most of the S. lycopersicum sesquiterpene synthases in stem trichomes. In addition, one sesquiterpene synthase was induced by jasmonic acid, while another appeared to be slightly repressed by the treatment. Our data provide a foundation to study the evolution of terpene synthases in cultivated and wild tomato