164 research outputs found
Future of Russia in export presentation
On the basis of the texts of British and American Mass Media hypothetical image of Russia in future is analyzed. Significant elements, that the authors are interested in, are singled out, these elements characterize the degree of detailing of the model of future.ΠΠ° ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π΅ ΡΠ΅ΠΊΡΡΠΎΠ² Π±ΡΠΈΡΠ°Π½ΠΎ-Π°ΠΌΠ΅ΡΠΈΠΊΠ°Π½ΡΠΊΠΈΡ
Π‘ΠΠ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΡΠ΅ΡΡΡ Π³ΠΈΠΏΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΎΠ±ΡΠ°Π· Π±ΡΠ΄ΡΡΠ΅ΠΉ Π ΠΎΡΡΠΈΠΈ. ΠΡΠ΄Π΅Π»ΡΡΡΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ, ΠΈΠ½ΡΠ΅ΡΠ΅ΡΡΡΡΠΈΠ΅ Π°Π²ΡΠΎΡΠΎΠ², Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ΅ΡΡΡ ΡΡΠ΅ΠΏΠ΅Π½Ρ Π΄Π΅ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π±ΡΠ΄ΡΡΠ΅Π³ΠΎ
Development of Lactococcus lactis Biosensors for Detection of Diacetyl
Some secondary metabolites of fermentative bacteria are desired compounds for the food industry. Examples of these compounds are diacetyl and acetaldehyde, which are produced by species of the lactic acid bacteria (LAB) family. Diacetyl is an aromatic compound, giving the buttery flavor associated with dairy products, and acetaldehyde is the compound responsible for the yogurt flavor and aroma. The quantification of these compounds in food matrices is a laborious task that involves sample preparation and specific analytical methods. The ability of bacteria to naturally sense metabolites has successfully been exploited to develop biosensors that facilitate the identification and quantification of certain metabolites (Mahr and Frunzke, 2016). The presence of a specific metabolite is sensed by the biosensors, and it is subsequently translated into the expression of one or more reporter genes. In this study we aimed to develop fluorescence-based biosensors to detect diacetyl and acetaldehyde. Since the metabolic pathways for production and degradation of these compounds are present in Lactococcus lactis, the sensing mechanisms in this bacterium are expected. Thus, we identified diacetyl and acetaldehyde responsive promoters by performing transcriptome analyses in L. lactis. The characterization of the biosensors showed their response to the presence of these compounds, and a further analysis of the diacetyl-biosensors (its dynamics and orthogonality) was performed. Moreover, we attempted to produce natural diacetyl from producer strains, namely L. lactis subsp. lactis biovar diacetylactis, to benchmark the performance of our biosensors. The diacetyl-biosensors responded linearly to the amounts of diacetyl obtained in the bacterial supernatants, i.e., the increases in GFP expression were proportional to the amounts of diacetyl present in the supernatants of L. lactis subsp. lactis biovar diacetylactis MR3-T7 strain. The biosensors developed in this study may eventually be used to engineer strains or pathways for increased diacetyl and acetaldehyde production, and may facilitate the detection of these metabolites in complex food matrices
Maturity model for assessing digitalization process of construction industry companies
The construction industry is on the verge of largeβscale changes as Industry 4.0 is preparing industrial enterprises for serious challenges. Digital transformation can bring significant changes in all areas of enterprise activity. Maturity models are usually used to assess the current situation and measure the digital maturity of an organization. The authorsβ four-stage model of digital maturity assessment for construction industry companies has been proposed. When developing the model, it had been decided to move away from general assessments and use specific changes that would occur at construction industry companies in the process of digital transformation as an assessment tool. The model contains fifteen assessment factors, changes of which at various stages are indicators of digital maturity. The proposed model contains a detailed description of the four stages of digital maturity, therefore it can serve not only to assess the digital maturity of industrial enterprises in the construction industry, but can also be used to indicate the goals planned to be achieved in the future as part of digital transformation, in strategies or business models of enterprises. A distinctive feature of the model is evaluation factors reflecting the degree of integration of the digital environment of industrial enterprises with BIM models of construction organizations
ΠΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²ΠΎ Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΡΠ½ΠΎ-ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅ΡΡ
Specific features of the communication space in the library and information sphere are discussed. The communication process players and factors are named. The communication space scheme for the library and information sphere is proposed. The scheme comprises: library and information staff, target audience (users), partners, and regulatory authorities. The formal and informal communication channels are characterized and compared with their advantages and drawbacks named. Several procedures for choosing communication channels relevant to target audience are proposed to increase effectiveness of the communication process and validity of communicant-recipient information transfer. Optimum communication channel selection stages and factors are characterized. Communication barriers due to wrong communication channel and their negative consequences for the communication process are described. Knowledge of the communication space in the library and information sphere and communication channel selection algorithm enables to exclude the communication barriers.Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΡΠ½ΠΎ-ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅ΡΡ. ΠΠ°Π·Π²Π°Π½Ρ ΡΡΠ°ΡΡΠ½ΠΈΠΊΠΈ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΈ ΡΠ°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΡ. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π° ΡΡ
Π΅ΠΌΠ° ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΡΠ½ΠΎ-ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅ΡΡ, ΠΊΠΎΡΠΎΡΠ°Ρ Π²ΠΊΠ»ΡΡΠ°Π΅Ρ Π² ΡΠ΅Π±Ρ Π΅Ρ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠΎΠ², ΡΠ΅Π»Π΅Π²ΡΡ Π°ΡΠ΄ΠΈΡΠΎΡΠΈΡ (ΡΠΈΡΠ°ΡΠ΅Π»Π΅ΠΉ), ΠΏΠ°ΡΡΠ½ΡΡΠΎΠ² ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠΈΠ΅ ΠΎΡΠ³Π°Π½Ρ. ΠΠ°Π½Π° ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΡΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ
ΠΈ Π½Π΅ΡΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΊΠ°Π½Π°Π»ΠΎΠ², ΡΠΊΠ°Π·Π°Π½Ρ ΠΈΡ
Π΄ΠΎΡΡΠΎΠΈΠ½ΡΡΠ²Π° ΠΈ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠΈ. Π Π΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°Π½Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ½Π½ΡΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ, ΡΠ²ΡΠ·Π°Π½Π½ΡΠ΅ Ρ Π²ΡΠ±ΠΎΡΠΎΠΌ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π΄Π»Ρ ΡΠ΅Π»Π΅Π²ΠΎΠΉ Π°ΡΠ΄ΠΈΡΠΎΡΠΈΠΈ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°Π»Π°, Ρ ΡΠ΅Π»ΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΈ ΠΊΠΎΡΡΠ΅ΠΊΡΠ½ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΡ ΠΎΡ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°Π½ΡΠ°. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΡΡΠ°ΠΏΡ Π²ΡΠ±ΠΎΡΠ° ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°Π»Π°. ΠΡΡΠ°ΠΆΠ΅Π½Ρ ΡΠ°ΠΊΡΠΎΡΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΡΡΠΈΡΡΠ²Π°ΡΡ ΠΏΡΠΈ Π²ΡΠ±ΠΎΡΠ΅ Π²ΠΎΡΡΡΠ΅Π±ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°Π»Π° ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΡ. Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΠ΅ Π±Π°ΡΡΠ΅ΡΡ, Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡΠΈΠ΅ ΠΏΡΠΈ Π½Π΅Π²Π΅ΡΠ½ΠΎ Π²ΡΠ±ΡΠ°Π½Π½ΠΎΠΌ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΊΠ°Π½Π°Π»Π΅ ΠΈ ΠΈΡ
Π½Π΅Π³Π°ΡΠΈΠ²Π½ΡΠ΅ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΠ²ΠΈΡ Π΄Π»Ρ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΈ. ΠΡΠΌΠ΅ΡΠ΅Π½ΠΎ: Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ΅ΠΌ, ΡΡΠΎ ΡΡΠ»ΠΎΠ²ΠΈΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ, ΠΏΡΠΈΠΎΡΠΈΡΠ΅ΡΡ ΠΈ Π½Π°ΡΡΡΠΎΠ΅Π½ΠΈΡ Π»ΡΠ΄Π΅ΠΉ Π½Π΅ΠΏΠΎΡΡΠΎΡΠ½Π½Ρ, Π²Π°ΠΆΠ½ΠΎ Π²ΠΎΠ²ΡΠ΅ΠΌΡ ΠΏΡΠΈΠ²Π½Π΅ΡΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΏΡΠΎΡΠ΅ΡΡ Ρ ΡΡΡΡΠΎΠΌ Π½ΠΎΠ²ΡΡ
ΠΎΠ±ΡΡΠΎΡΡΠ΅Π»ΡΡΡΠ². ΠΠΎΠ΄ΡΡΡΠΊΠ½ΡΡΠΎ, ΡΡΠΎ Π·Π½Π°Π½ΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΡΠ½ΠΎ-ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅ΡΡ ΠΈ Π°Π»Π³ΠΎΡΠΈΡΠΌΠ° Π²ΡΠ±ΠΎΡΠ° ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΡΡΠ΅Π³ΠΎ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΊΠ°Π½Π°Π»Π° ΠΏΠΎΠΌΠΎΠΆΠ΅Ρ ΠΈΠ·Π±Π΅ΠΆΠ°ΡΡ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΡΡ
Π±Π°ΡΡΠ΅ΡΠΎΠ²
Some implementation issues of the bologna process in the russian federation
Π Π°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° Π½Π΅ΡΠ°Π²Π΅Π½ΡΡΠ²Π° Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈ ΠΏΠΎΡΠΎΠΆΠ΄Π°Π΅ΠΌΡΠ΅ ΠΠΎΠ»ΠΎΠ½ΡΠΊΠΈΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠΌ ΡΠ»ΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ°ΠΌΠΎΡΡΠΎΡΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ²The article is devoted to the problem of inequality in education and the difficulties in realizing the independent work of students, generated by the Bologna proces
Religion as Source Domain of Metaphors in World War II Media Discourse
The article studies the English-language media discourses of the World War II period from a retrospective point of view. The aim is to identify the patterns of modeling the images of war and peace in British, American and Australian media discourses. The definition of military media discourse as an institutional form of communication is proposed, its essential characteristics are highlighted. The source of the material was the authoritative digitized archives of the UK, the USA and Australia. The material was extracted using corpus linguistics tools. The qualitative analysis was carried out within the framework of the cognitive-discourse methodology using the method of metaphorical modeling. The object of the research is the dominant metaphor used when representing the images of war and peace in the three discourses. The authors show common and specific features of the use of the religious metaphor, conditioned by cultural and extra-linguistic factors, and conclude that the religious metaphor places war and peace at different poles of the scale of values and actualizes the binary axiological opposition βgood β evilβ, βlight β darknessβ
Formation of an intelligent control system in the field of electric power industry based on the technological development of power supply components
The relevance of the paper is determined by the fact that the digital transformation of the country is recognized as one of the priorities for the economic development of the state. It is necessary to remove barriers for the digital transformation in the most promising areas of production and life by stimulating the introduction of digital technologies and attracting investments, overcoming the digital divide, deepening cooperation between states in the digital sphere and developing the country's digital innovation infrastructure. The novelty of the study is determined by the fact that the transition to a new level of digital functioning of electric systems is possible in the context of formation of a smart energy supply system and the introduction of modern information technologies. The authors show that the introduction of new energy meters is the basis for creating an innovative infrastructure of the fuel and energy complex of the national economy. The authors believe that this is the basis for the transition to a new level of energy supply and the introduction of new economic mechanisms for demand management and further development of energy markets. This in turn contributes to the integration of energy systems of different countries. The practical significance of the study is determined by the possibilities of stratification of the economic assessment of the energy industry and the formation of a sustainable assessment of the formation of energy supply systems. It is proposed to determine the needs of industry in sustainable energy supply
Tapping into rhythm generation circuitry in humans during simulated weightlessness conditions
An ability to produce rhythmic activity is ubiquitous for locomotor pattern generation and modulation. The role that the rhythmogenesis capacity of the spinal cord plays in injured populations has become an area of interest and systematic investigation among researchers in recent years, despite its importance being long recognized by neurophysiologists and clinicians. Given that each individual interneuron, as a rule, receives a broad convergence of various supraspinal and sensory inputs and may contribute to a vast repertoire of motor actions, the importance of assessing the functional state of the spinal locomotor circuits becomes increasingly evident. Air-stepping can be used as a unique and important model for investigating human rhythmogenesis since its manifestation is largely facilitated by a reduction of external resistance. This article aims to provide a review on current issues related to the βlocomotorβ state and interactions between spinal and supraspinal influences on the central pattern generator circuitry in humans, which may be important for developing gait rehabilitation strategies in individuals with spinal cord and brain injuries
Simulation of alkylation products processing to study the duration of period between reactor washings
Riboflavin Biosynthesis and Overproduction by a Derivative of the Human Gut Commensal Bifidobacterium longum subsp. infantis ATCC 15697
Riboflavin or vitamin B2 is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Despite increased interest in microbial synthesis of this water-soluble vitamin, the metabolic pathway for riboflavin biosynthesis has been characterized in just a handful of bacteria. Here, comparative genome analysis identified the genes involved in the de novo biosynthetic pathway of riboflavin in certain bifidobacterial species, including the human gut commensal Bifidobacterium longum subsp. infantis (B. infantis) ATCC 15697. Using comparative genomics and phylogenomic analysis, we investigated the evolutionary acquisition route of the riboflavin biosynthesis or rib gene cluster in Bifidobacterium and the distribution of riboflavin biosynthesis-associated genes across the genus. Using B. infantis ATCC 15697 as model organism for this pathway, we isolated spontaneous riboflavin overproducers, which had lost transcriptional regulation of the genes required for riboflavin biosynthesis. Among them, one mutant was shown to allow riboflavin release into the medium to a concentration of 60.8 ng mLβ1. This mutant increased vitamin B2 concentration in a fecal fermentation system, thus providing promising data for application of this isolate as a functional food ingredient
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