Forest maturity has a stronger influence on the prevalence of spider monkeys than howler monkeys in an anthropogenically impacted rainforest landscape

[EN] The transformation and depletion of primary forest over the past few decades have placed almost half of the world's primate species under the threat of extinction. Developing any successful conservation program for primates requires distribution and demography data, as well as an understanding of the relationships between these factors and their habitat. Between March and June 2010 and 2011 we collected data on the presence and demographic parameters of howler and spider monkeys by carrying out surveys, and validated our findings using local knowledge. We then examined the relationship between forest type and the presence of these primates at 54 sites in the northern area of the Selva Zoque Corridor, Mexico. We detected 86 spider monkey groups across 31 plots and censused 391 individuals (mean +/- SD = 5.9 +/- 3.0 individuals per sub-group, n = 67 sub-groups). We also detected 69 howler monkey groups across 30 plots and censused 117 individuals (mean +/- SD = 5.3 +/- 2.4 individuals per group, n = 22 groups). Howler monkey presence was not related to any specific vegetation type, while spider monkeys were present in areas with a higher percentage of tall forest (trees > 25 m high). Overall, spider monkeys were more prevalent than howler monkeys in our sampling sites and showed demographic characteristics similar to those in better protected areas, suggesting that the landscape features in the Uxpanapa Valley are suitable for their needs. Conversely, howler monkey presence was found to be more limited than in other regions, possibly due to the extended presence of spider monkeys.We thank the National Council of Science of Technology (CONACyT) and the Veracruz State Government for providing financial support to the project (grant no. 108990). ASG received support from Bournemouth University, the Centro de Investigaciones Tropicales and CONACyT (registration 195409) to conduct all the data analysis as part of her PhD. JCD received funding from the Isaac Newton Trust. We thank C. A. Munoz-Robles for providing the base map of the study site. We are very grateful to the communities of the Uxpanapa Valley for helping us throughout the development of this project. We are grateful to Dr Bicca-Marques and to two anonymous reviewers for their comments, which greatly helped in improving this manuscript. This study complied with the legal requirements of Mexico (SEMARNAT-DGVS/03660/11) and was approved by Universidad Veracruzana

MALDI Profiling of Human Lung Cancer Subtypes

Proteomics is expected to play a key role in cancer biomarker discovery. Although it has become feasible to rapidly analyze proteins from crude cell extracts using mass spectrometry, complex sample composition hampers this type of measurement. Therefore, for effective proteome analysis, it becomes critical to enrich samples for the analytes of interest. Despite that one-third of the proteins in eukaryotic cells are thought to be phosphorylated at some point in their life cycle, only a low percentage of intracellular proteins is phosphorylated at a given time.In this work, we have applied chromatographic phosphopeptide enrichment techniques to reduce the complexity of human clinical samples. A novel method for high-throughput peptide profiling of human tumor samples, using Parallel IMAC and MALDI-TOF MS, is described. We have applied this methodology to analyze human normal and cancer lung samples in the search for new biomarkers. Using a highly reproducible spectral processing algorithm to produce peptide mass profiles with minimal variability across the samples, lineal discriminant-based and decision tree–based classification models were generated. These models can distinguish normal from tumor samples, as well as differentiate the various non–small cell lung cancer histological subtypes.A novel, optimized sample preparation method and a careful data acquisition strategy is described for high-throughput peptide profiling of small amounts of human normal lung and lung cancer samples. We show that the appropriate combination of peptide expression values is able to discriminate normal lung from non-small cell lung cancer samples and among different histological subtypes. Our study does emphasize the great potential of proteomics in the molecular characterization of cancer

Forest maturity has a stronger influence on the prevalence of spider monkeys than howler monkeys in an anthropogenically impacted rainforest landscape.

Funder: Bournemouth UniversityThe transformation and depletion of primary forest over the past few decades have placed almost half of the world's primate species under the threat of extinction. Developing any successful conservation program for primates requires distribution and demography data, as well as an understanding of the relationships between these factors and their habitat. Between March and June 2010 and 2011 we collected data on the presence and demographic parameters of howler and spider monkeys by carrying out surveys, and validated our findings using local knowledge. We then examined the relationship between forest type and the presence of these primates at 54 sites in the northern area of the Selva Zoque Corridor, Mexico. We detected 86 spider monkey groups across 31 plots and censused 391 individuals (mean ± SD = 5.9 ± 3.0 individuals per sub-group, n = 67 sub-groups). We also detected 69 howler monkey groups across 30 plots and censused 117 individuals (mean ± SD = 5.3 ± 2.4 individuals per group, n = 22 groups). Howler monkey presence was not related to any specific vegetation type, while spider monkeys were present in areas with a higher percentage of tall forest (trees > 25 m high). Overall, spider monkeys were more prevalent than howler monkeys in our sampling sites and showed demographic characteristics similar to those in better protected areas, suggesting that the landscape features in the Uxpanapa Valley are suitable for their needs. Conversely, howler monkey presence was found to be more limited than in other regions, possibly due to the extended presence of spider monkeys