Differentiation of memory T cells to virus plaque-forming cells and cytotoxic T lymphocytes

The aims of this study were to define the T-cell subpopulation(s) detected by the virus plaque assay, and particularly to determine whether the virus plaque assay could be used to enumerate cytotoxic T lymphocytes. In addition, studies were undertaken to ascertain whether cell proliferation was required for development of cytotoxic effector function and virus plaque formation by these subpopulations. The results of experiments with a secondary mouse mixed lymphocyte culture (MLC) model indicated that 70 percent of virus plaque-forming cells bore the Ly 1 phenotype and 30 percent the Ly 2,3 phenotype. Three lines of evidence suggested that cytotoxic T lymphocytes (CTL) can be detected by this assay: the fact that some virus plaque-forming cells (V-PFC) bear the same Ly phenotype as CTL; the use of an inhibitor of DNA synthesis indicated that proliferating cells could be eliminated with no effect on V-PFC production and cytotoxic activity of the Ly 2,3 cell population; and that infection of primed lymphocyteswith vesicular stomatitis virus before (MLC) stimulation eliminated cytotoxic activity. In primary MLC, development of V-PFC and CTL was completely abolished by cytosine arabinoside. In contrast, in secondary MLC, some CTL and V- PFC were generated by antigenic stimulation in the absence of proliferation. However, the development of both functions became progressively more susceptible to cytosine arabinoside as the time between primary immunization and in vitro boosting is increased. It is suggested that there may be a considerable disparity between the number of existing effector cells at any given time and the cytotoxic potential, i.e. the number of cells capable of being generated by antigenic stimulation

The Emerging Aversion to Inequality: Evidence from Poland 1992-2005

This paper provides an illustration of the changing tolerance for inequality in a context of radical political and economic transformation and rapid economic growth. We focus on the Polish experience of transition and explore self-declared attitudes of the citizens. Using monthly representative surveys of the population, realized by the Polish poll institute (CBOS) from 1992 to 2005, we identify a structural break in the relation between income inequality and subjective evaluation of well-being. The downturn in the tolerance for inequality (1997) coincides with the increasing distrust of political elites.http://deepblue.lib.umich.edu/bitstream/2027.42/64387/1/wp919.pd

Surface ozone variability at Kislovodsk Observatory

The results of the surface ozone observations at the Observatory 'Kislovodsk', situated in the North Caucasus at the altitude 2070 m a.s.l., are given. The observatory is in the background conditions and the variations of the surface ozone are determined by the natural dynamic and photochemical processes. The mean value of the concentration and its seasonal variations are very near to those obtained at the high-mountain stations in Alps. The daily variations have the features, which remain stable during all warm period of the year (April-October). These features, including the minimum of the surface ozone at noon, are formed by the mountain-valley circulation. The significant variations of the surface ozone are connected with the unstationary lee waves

Discovery of an unusual bright eclipsing binary with the longest known period: TYC 2505-672-1 / MASTER OT J095310.04+335352.8

We report on the MASTER Global Robotic Net discovery of an eclipsing binary, MASTER OT J095310.04+335352.8, previously known as unremarkable star TYC 2505-672-1, which displays extreme orbital parameters. The orbital period P=69.1 yr is more than 2.5 times longer than that of epsilon-Aurigae, which is the previous record holder. The light curve is characterized by an extremely deep total eclipse with a depth of more than 4.5 mag, which is symmetrically shaped and has a total duration of 3.5 yrs. The eclipse is essentially gray. The spectra acquired with the Russian 6 m BTA telescope both at minimum and maximum light mainly correspond to an M0-1III--type red giant, but the spectra taken at the bottom of eclipse show small traces of a sufficiently hot source. The observed properties of this system can be better explained as the red giant eclipsed by a large cloud (the disk) of small particles surrounding the invisible secondary companion.Comment: 8 figures, 9 pages, Astronomy and astrophysics in prin

Surface ozone at the Caucasian site Kislovodsk High Mountain Station and the Swiss Alpine site Jungfaujoch: data analysis and trends (1990–2006)

Long-term ozone measurements of two background mountain sites, namely the Kislovodsk High Mountain Station in Caucasus, Russia (KHMS, 43.70° N, 42.70° E, 2070 m a.s.l.) and the Jungfraujoch in Switzerland (JFJ, 46.55° N, 7.98° E, 3580 m a.s.l.) are compared. Despite of more than 1.5 km altitude difference ozone mixing ratios are comparable at JFJ an KHMS in the beginning of measurements (1990–1993) while the annually averaged levels at JFJ are around 15 ppb higher than the ones at KHMS for the most recent years (1997–2006). The seasonal cycle of the surface ozone mixing ratios is characterized by a double spring-summer maximum at both sites with a spring one being more pronounced for the air masses with the longest contact with the upper free troposphere and stratosphere. Ozone mixing ratio increased at JFJ but decreased at KHMS for the period 1990–2006. Trends are more pronounced for the 1990s (+0.73±0.20 ppb/year at JFJ and −0.91±0.17 ppb/year at KHMS for the period 1991–2001) in comparison with the later years (+0.04±0.21 ppb/year at JFJ and −0.37±0.14 ppb/year at KHMS for the period 1997–2006). Trends show a distinct seasonality, which is different for the different periods. To investigate possible reasons for this remarkable trends difference 3-D trajectories using LAGRANTO trajectory model are used. Effects of horizontal and vertical transport on ozone trends are considered. No substantial systematic changes in the transport patterns were detected which could lead to strong changes in the trend magnitude between 1991–2001 and 1997–2006. The geographical position of the sites relative to the main topographic features and emission sources as well as distance from the coast are interpreted to be among the main reasons for the opposite surface ozone trends. During the 90s the JFJ trend reflects increase of the ozone in the upper free troposphere/lower stratosphere, while KHMS is not sensitive to this change or even showing the opposite tendency. The analysis provided evidence for a stronger influence of processes in the lower troposphere, in particular the dramatic emission decrease in the earlier 1990s in former USSR and emissions regulations in Western Europe on the surface ozone trend at KHMS.ISSN:1680-7375ISSN:1680-736

Photometric observations of the supernova 2009nr

We present the results of our UBVRI CCD photometry for the second brightest supernova of 2009, SN 2009nr, discovered during a sky survey with the telescopes of the MASTER robotic network. Its light and color curves and bolometric light curves have been constructed. The light-curve parameters and the maximum luminosity have been determined. SN 2009nr is shown to be similar in light-curve shape and maximum luminosity to SN 1991T, which is the prototype of the class of supernovae Ia with an enhanced luminosity. SN 2009nr exploded far from the center of the spiral galaxy UGC 8255 and most likely belongs to its old halo population. We hypothesize that this explosion is a consequence of the merger of white dwarfs

Measurements of the total column amount of NO2 at Kislovodsk Observatory in 1979 - 1990

The results of the measurements of the NO2 total content at the 'Kislovodsk' observatory (North Caucasus 2070 m a.s.l.) in 1979-1990 are presented. The measurements were based on the absorption of the direct sunlight in the visible region of the spectrum. The variation of the NO2 content from the morning to the evening and the seasonal and year to year variations are analyzed. The less amplitude of the daily variations of the NO2 total content in comparison with the variations of the stratospheric NO2 content says about the significant insert of the tropospheric and lower stratospheric NO2 in its total content. The year to year variations of NO2 and ozone are in antiphase. Their values depends on the regional features of the atmospheric circulation. The quasibiennial oscillations of the total NO2 content are picked out