Ultrafast sensing of photoconductivity decay using microwave resonators

Microwave reflectance probed photoconductivity (or $\mu$-PCD) measurement represents a contactless and non-invasive method to characterize impurity content in semiconductors. Major drawbacks of the method include a difficult separation of reflectance due to dielectric and conduction effects and that the $\mu$-PCD signal is prohibitively weak for highly conducting samples. Both of these limitations could be tackled with the use of microwave resonators due to the well-known sensitivity of resonator parameters to minute changes in the material properties combined with a null measurement. A general misconception is that time resolution of resonator measurements is limited beyond their bandwidth by the readout electronics response time. While it is true for conventional resonator measurements, such as those employing a frequency sweep, we present a time-resolved resonator parameter readout method which overcomes these limitations and allows measurement of complex material parameters and to enhance $\mu$-PCD signals with the ultimate time resolution limit being the resonator time constant. This is achieved by detecting the transient response of microwave resonators on the timescale of a few 100 ns \emph{during} the $\mu$-PCD decay signal. The method employs a high-stability oscillator working with a fixed frequency which results in a stable and highly accurate measurement.Comment: 7 pages, 6 figures+Supplementary Material

Extracelluláris vezikulák és hematológiai malignitásokban játszott szerepük

Absztrakt Extracelluláris vesiculák minden szervezetben képződnek. Három legintenzívebben vizsgált csoportjuk az apoptotikus testek, a microvesiculák és az exosomák. A sejtek közötti kommunikációban, immunreakciókban, angiogenezisben betöltött szerepük csak néhány az eddig megismertek közül. A fiziológiás folyamatok mellett sokféle betegségben leírták változásaikat; a patomechanizmusban betöltött szerepük mellett felvetődik potenciális használatuk biomarkerekként. A szerzők betekintést kívánnak nyújtani az extracelluláris vesiculák kutatásába, kiemelve azt a néhány tanulmányt, amely a hematológiai malignitásokra fókuszált. A microvesiculák és exosomák vérplazmában mért mennyisége, a terápia során megfigyelt minőségi változása miatt felmerült, hogy a diagnosztikában, prognosztikában, illetve a minimális residualis betegség monitorozásában is használhatók lehetnek. Akut myeloid leukaemiában a természetes ölősejtek aktivitásának szupresszálásában bizonyított a blasteredetű exosomák szerepe. Krónikus lymphoid leukaemiában a microvesiculák közreműködése valószínű a gyógyszer-rezisztencia kialakulásában is. Orv. Hetil., 2016, 157(35), 1379–1384. | Abstract Extracellular vesicles are produced in all organisms. The most intensively investigated categories of extracellular vesicles include apoptotic bodies, microvesicles and exosomes. Among a very wide range of areas, their role has been confirmed in intercellular communication, immune response and angiogenesis (in both physiological and pathological conditions). Their alterations suggest the potential use of them as biomarkers. In this paper the authors give an insight into the research of extracellular vesicles in general, and then focus on published findings in hematological malignancies. Quantitative and qualitative changes of microvesicles and exosomes may have value in diagnostics, prognostics and minimal residual disease monitoring of hematological malignancies. The function of extracellular vesicles in downregulation of natural killer cells’ activity has been demonstrated in acute myeloid leukemia. In chronic lymphocytic leukemia, microvesicles seem to play a role in drug resistance. Orv. Hetil., 2016, 157(35), 1379–1384

Tidally locked rotation of the dwarf planet (136199) Eris discovered from long-term ground based and space photometry

The rotational states of the members in the dwarf planet - satellite systems in the transneptunian region are determined by the formation conditions and the tidal interaction between the components, and these rotational characteristics are the prime tracers of their evolution. Previously a number of authors claimed highly diverse values for the rotation period for the dwarf planet Eris, ranging from a few hours to a rotation (nearly) synchronous with the orbital period (15.8 d) of its satellite, Dysnomia. In this letter we present new light curve data of Eris, taken with $\sim$1-2m-class ground based telescopes, and with the TESS and Gaia space telescopes. TESS data could not provide a well-defined light curve period, but could constrain light curve variations to a maximum possible light curve amplitude of $\Delta m$ $\leq$ 0.03 mag (1-$\sigma$) for P $\leq$ 24 h periods. Both the combined ground-based data and the Gaia measurements unambiguously point to a light curve period equal to the orbital period of Dysnomia, P = 15.8 d, with a light curve amplitude of $\Delta m$ $\approx$ 0.03 mag, i.e. the rotation of Eris is tidally locked. Assuming that Dysnomia has a collisional origin, calculations with a simple tidal evolution model show that Dysnomia has to be relatively massive (mass ratio of q = 0.01--0.03) and large (radius of $R_s$ $\geq$ 300 km) to slow down Eris to synchronized rotation. These simulations also indicate that -- assuming tidal parameters usually considered for transneptunian objects -- the density of Dysnomia should be 1.8-2.4 $g cm^{-3}$, an exceptionally high value among similarly sized transneptunian objects, putting important constraints on the formation conditions.Comment: Accepted for publication in Astronomy and Astrophysics Letters, data of tables A.1, A.2 and A.4 are available at https://cloud.konkoly.hu/s/ESiKi4GZyifJmj

Pro- and anti-inflammatory factors, vascular stiffness and outcomes in chronic hemodialysis patients

In this observational study we addressed accelerated arteriosclerosis (AS) in patients with chronic renal failure (CRF) on hemodialysis (HD) by measuring vascular stiffness (VS) parameters and attempted to relate them to pro-inflammatory and protective factors.96 consecutive patients receiving regular HD were included. 20 adult patients without major renal, cardiovascular or metabolic morbidities served as controls.AS parameters (carotid-femoral pulse wave velocity - PWV, aortic augmentation index - Aix) were measured by using applanation tonometry (SphygmoCor, AtCor Medical, Sidney). In addition to routine laboratory tests 25(OH) vitamin D3 (vitamin D3) and high-sensitivity C-reactive protein (hsCRP) were quantified by immunometric assay; whereas fetuin-A, α-Klotho, tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) were determined by ELISA.Pro-inflammatory biomarkers (hsCRP, TNF-α and TGF-β1) were markedly elevated (P < 0.01), while anti-inflammatory factors (fetuin-A: P < 0.05, α-Klotho: P < 0.01, vitamin D3: P < 0.01) significantly depressed in HD patients when compared to controls. PWV was significantly affected only by total cholesterol, fetuin-A and dialysis time. Multiple linear regression analyses revealed that several clinical and laboratory parameters were associated with pro- and anti-inflammatory biomarkers rather than VS. The impact of baseline clinical and biochemical variables on outcome measures were also analyzed after three-year follow-up, and it was demonstrated that low levels of vitamin D, α-Klotho protein and fetuin-A were related to adverse cardiovascular outcomes, whereas all-cause mortality was associated with elevated hsCRP and depressed vitamin D.Our results provide additional information on the pathomechanism of accelerated AS in patients with CRF, and documented direct influence of pro- and anti-inflammatory biomarkers on major outcome measures

Új és hagyományos irányok a gyermekkori akut lymphoblastos leukaemia biológiájában és ellátásában

Owing to clinical trials and improvement over the past few decades, the majority of children with acute lymphoblastic leukemia (ALL) survive by first-line chemotherapy and combat with the problems of returning to community. However, many patients may have severe acute or late therapeutic side effects, and the survival rate in some groups (e.g., patients with MLL rearrangements, hypodiploidy, IKZF1 mutation or early precursor T cell phenotype) is far behind the average. Innovative strategies in medical attendance provide better clinical outcomes for them: complete gene diagnostics, molecularly targeted anticancer treatment, immuno-oncology and immune cell therapy. The number of genes with identified alterations in leukemic lymphoblasts is over thirty and their pathobiologic role is only partly clear. There are known patient groups where the use of specific drugs is based on gene expression profiling (e.g., tyrosine kinase inhibitors in Philadelphia-like B-cell ALL). The continuous assessment of minimal residual disease became a routine due to the determination of a leukemia-associated immunophenotype by flow cytometry or a sensitive molecular marker by molecular genetics at diagnosis. Epitopes of cluster differentiation antigens on blast surface (primarily CD19, CD20 and CD22 on malignant B cells) can be attacked by monoclonal antibodies. Moreover, antitumor immunity can be strengthened utilizing either cell surface markers (bispecific T cell engagers, chimeric antigen receptor T cell therapy) or tumor-specific immune cells (immune checkpoint inhibitors). This review gives an insight into current knowledge in these innovative therapeutic directions. Orv Hetil. 2018; 159(20): 786-797

Complexity of equational theory of relational algebras with standard projection elements

The class $\mathsf{TPA}$ of t rue p airing a lgebras is defined to be the class of relation algebras expanded with concrete set theoretical projection functions. The main results of the present paper is that neither the equational theory of $\mathsf{TPA}$ nor the first order theory of $\mathsf{TPA}$ are decidable. Moreover, we show that the set of all equations valid in $\mathsf{TPA}$ is exactly on the $\Pi ^1_1$ level. We consider the class $\mathsf{TPA}^-$ of the relation algebra reducts of $\mathsf{TPA}$’s, as well. We prove that the equational theory of $\mathsf{TPA}^-$ is much simpler, namely, it is recursively enumerable. We also give motivation for our results and some connections to related work

Tidally locked rotation of the dwarf planet (136199) Eris discovered via long-term ground-based and space photometry

This is an Open Access article, published by EDP Sciences, under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.The rotational states of the members in the dwarf planet-satellite systems in the trans-Neptunian region are determined by formation conditions and the tidal interaction between the components. These rotational characteristics serve as prime tracers of their evolution. A number of authors have claimed a very broad range of values for the rotation period for the dwarf planet Eris, ranging from a few hours to a rotation that is (nearly) synchronous with the orbital period (15.8 d) of its satellite, Dysnomia. In this Letter, we present new light curve data for Eris, taken with ∼1–2 m-class ground based telescopes and with the TESS and Gaia space telescopes. The TESS data did not provide a well-defined light curve period, but it could be used to constrain light curve variations to a maximum possible light curve amplitude of Δm ≤ 0.03 mag (1-σ) for P ≤ 24 h periods. Both the combined ground-based data and Gaia measurements unambiguously point to a light curve period equal to the orbital period of Dysnomia, P = 15.8 d, with a light curve amplitude of Δm ≈ 0.03 mag, indicating that the rotation of Eris is tidally locked. Assuming that Dysnomia has a collisional origin, calculations with a simple tidal evolution model show that Dysnomia must be relatively massive (mass ratio of q = 0.01–0.03) and large (radius of Rs ≥ 300 km) to have the potential to slow Eris down to a synchronised rotation. These simulations also indicate that (assuming tidal parameters usually considered for trans-Neptunian objects) the density of Dysnomia should be 1.8–2.4 g cm−3. This is an exceptionally high value among similarly sized trans-Neptunian objects, setting important constraints on their formation conditions. © The Authors 2023.The research leading to these results has received funding from the K-138962 grant of the National Research, Development and Innovation Office (NKFIH, Hungary). The data presented in this Letter were obtained from the Mikulski Archive for Space Telescopes (MAST). STScI is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS5-26555. Support for MAST for non-HST data is provided by the NASA Office of Space Science via grant NNX09AF08G and by other grants and contracts. This research has made use of data and services provided by the International Astronomical Union’s Minor Planet Center. Part of the funding for GROND (both hardware as well as personnel) was generously granted from the Leibniz-Prize to Prof. G. Hasinger (DFG grant HA 1850/28-1). We are grateful to the CAHA and OSN staff. This research is partially based on observations collected at the Centro Astronómico Hispano Alemán (CAHA) at Calar Alto, operated jointly by Junta de Andalucía and Consejo Superior de Investigaciones Científicas (IAA-CSIC). This research was also partially based on observation carried out at the Observatorio de Sierra Nevada (OSN) operated by Instituto de Astrofísica de Andalucía (CSIC). P.S-S. acknowledges financial support by the Spanish grant AYA-RTI2018-098657-J-I00 “LEO-SBNAF”(MCIU/AEI/FEDER, UE). P.S-S., J.L.O., N.M., and R.D. acknowledge financial support from the State Agency for Research of the Spanish MCIU through the “Center of Excellence Severo Ochoa” award for the Instituto de Astrofísica de Andalucía (SEV-2017-0709), they also acknowledge the financial support by the Spanish grants AYA-2017-84637-R and PID2020-112789GB-I00, and the Proyectos de Excelencia de la Junta de Andalucía 2012-FQM1776 and PY20-01309. This work made use of Astropy: (http://www.astropy.org) a community-developed core Python package and an ecosystem of tools and resources for astronomy (Astropy Collaboration 2013, 2018, 2022). This work has made use of data from the European Space Agency (ESA) mission Gaia (https://www.cosmos.esa.int/gaia), processed by the Gaia Data Processing and Analysis Consortium (DPAC, https://www.cosmos.esa.int/web/gaia/dpac/consortium). Funding for the DPAC has been provided by national institutions, in particular the institutions participating in the Gaia Multilateral Agreement.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).Peer reviewe

Discovery of nucleotide polymorphisms in the Musa gene pool by Ecotilling

Musa (banana and plantain) is an important genus for the global export market and in local markets where it provides staple food for approximately 400 million people. Hybridization and polyploidization of several (sub)species, combined with vegetative propagation and human selection have produced a complex genetic history. We describe the application of the Ecotilling method for the discovery and characterization of nucleotide polymorphisms in diploid and polyploid accessions of Musa. We discovered over 800 novel alleles in 80 accessions. Sequencing and band evaluation shows Ecotilling to be a robust and accurate platform for the discovery of polymorphisms in homologous and homeologous gene targets. In the process of validating the method, we identified two single nucleotide polymorphisms that may be deleterious for the function of a gene putatively important for phototropism. Evaluation of heterozygous polymorphism and haplotype blocks revealed a high level of nucleotide diversity in Musa accessions. We further applied a strategy for the simultaneous discovery of heterozygous and homozygous polymorphisms in diploid accessions to rapidly evaluate nucleotide diversity in accessions of the same genome type. This strategy can be used to develop hypotheses for inheritance patterns of nucleotide polymorphisms within and between genome types. We conclude that Ecotilling is suitable for diversity studies in Musa, that it can be considered for functional genomics studies and as tool in selecting germplasm for traditional and mutation breeding approaches