Cyanobacteria vs green algae:which group has the edge?

The dogma surrounding carbon assimilation has it that, due to their highly effective CO2-concentrating mechanisms, cyanobacteria will always out-perform, for example, green algae where inorganic carbon is in short supply. Working on the cyanobacterial genus Microcystis, Ji et al. (2017) now suggest this might not always be true, with possible improved performance with rises in atmospheric (and hence dissolved) CO2. Many cyanobacteria form extensive toxic blooms that present significant health risks and economic costs: how they will react in a future world with elevated CO2 and temperature is thus of intense interest for water management

FLASH redshift survey - I. Observations and Catalogue

The FLAIR Shapley-Hydra (FLASH) redshift survey catalogue consists of 4613 galaxies brighter than \bJ = 16.7 (corrected for Galactic extinction) over a 605 sq. degree region of sky in the general direction of the Local Group motion. The survey region is an approximately 60\degr \times 10\degr strip spanning the sky from the Shapley Supercluster to the Hydra cluster, and contains 3141 galaxies with measured redshifts. Designed to explore the effect of the galaxy concentrations in this direction (in particular the Supergalactic plane and the Shapley Supercluster) upon the Local Group motion, the 68% completeness allows us to sample the large-scale structure better than similar sparsely-sampled surveys. The survey region does not overlap with the areas covered by ongoing wide-angle (Sloan or 2dF) complete redshift surveys. In this paper, the first in a series, we describe the observation and data reduction procedures, the analysis for the redshift errors and survey completeness, and present the survey data.Comment: 12 pages, 12 figures, mn.sty, submitted to MNRA

Evolutionary temperature compensation of carbon fixation in marine phytoplankton

The efficiency of carbon sequestration by the biological pump could decline in the coming decades because respiration tends to increase more with temperature than photosynthesis. Despite these differences in the short-term temperature sensitivities of photosynthesis and respiration, it remains unknown whether the long-term impacts of global warming on metabolic rates of phytoplankton can be modulated by evolutionary adaptation. We found that respiration was consistently more temperature dependent than photosynthesis across 18 diverse marine phytoplankton, resulting in universal declines in the rate of carbon fixation with short-term increases in temperature. Long-term experimental evolution under high temperature reversed the short-term stimulation of metabolic rates, resulting in increased rates of carbon fixation. Our findings suggest that thermal adaptation may therefore have an ameliorating impact on the efficiency of phytoplankton as primary mediators of the biological carbon pump

Non-Skeletal Biomineralization by Eukaryotes: Matters of Moment and Gravity

Skeletal biomineralisation by microbial eukaryotes significantly affects the global biogeochemical cycles of carbon, silicon and calcium. Non-skeletal biomineralisation by eukaryotic cells, with precipitates retained within the cell interior, can duplicate some of the functions of skeletal minerals, e.g., increased cell density, but not the mechanical and antibiophage functions of extracellular biominerals. However, skeletal biomineralisation does not duplicate many of the functions of non-skeletal biominerals. These functions include magnetotaxis (magnetite), gravity sensing (intracellular barite, bassanite, celestite and gypsum), buffering and storage of elements in an osmotically inactive form (calcium as carbonate, oxalate, polyphosphate and sulfate; phosphate as polyphosphate) and acid-base regulation, disposing of excess hydroxyl ions via an osmotically inactive product (calcium carbonate, calcium oxalate). Although polyphosphate has a wide phylogenetic distribution among microbial eukaryotes, other non-skeletal minerals have more restricted distributions, and as yet there seems to be no definitive evidence that the alkaline earth components (Ba and Sr) of barite and celestite are essential for completion of the life cycle in organisms that produce these minerals.Organismic and Evolutionary Biolog

The Potential effect of Low Cell Osmolarity on Cell Function through decreased concentration of enzyme substrates

© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. Some freshwater algae have lower (180 osmol m −3 ). Low osmolarities are related to the presence of flagella and the low energy cost of active water efflux following downhill water influx unconstrained by cell walls covering the plasmalemma, and the low resource cost of cell wall synthesis with the same mechanical degree of safety. One consequence of low intracellular osmolarity is limitation on the concentration of metabolites, that is, substrates and products of enzyme activity. Models of the flux through metabolic pathways, and hence the specific growth rate, using steady-state concentrations of enzymes and metabolites have involved organisms with intracellular metabolite osmolarities >280 osmol m −3 , where the metabolite concentrations are much greater than the total osmolarity of some freshwater algae. Since the protein concentration (mol m −3 ) in the cells and the specific growth rates of freshwater cells with low and with higher intracellular osmolarity are highly similar, the models of trade-offs between enzyme and metabolite concentrations for cells with high intracellular osmolarity need modification for cells with low intracellular osmolarity. The soluble free-radical scavenger ascorbate can constitute as little as 0.2% of the low intracellular metabolite concentration (mol m −3 ) of low-intracellular-osmolarity cells

Making the most of community energies:Three perspectives on grassroots innovation

Grassroots innovations for sustainability are attracting increasing policy attention. Drawing upon a wide range of empirical research into community energy in the UK, and taking recent support from national government as a case study, we apply three distinct analytical perspectives: strategic niche management; niche policy advocacy; and critical niches. Whilst the first and second perspectives appear to explain policy influence in grassroots innovation adequately, each also shuts out more transformational possibilities. We therefore argue that, if grassroots innovation is to realise its full potential, then we need to also pursue a third, critical niches perspective, and open up debate about more socially transformative pathways to sustainability

Implementation of the partograph in India's JSY cash transfer programme for facility births: a mixed methods study in Madhya Pradesh province

Objectives To study implementation of partograph use to monitor labour in facilities providing the JSY (Janani Suraksha Yojana) cash transfer programme for facility births in India by determining (1) adherence to partograph use, (2) staff abilities at partograph use and (3) staff responsiveness to the policy on partograph use. Design A mixed methods study using Carroll's framework for implementation fidelity. Methods include (1) obstetric case record review, (2) a vignette-based survey among nurse midwives and (3) interviews with staff. Setting Routine use of the partograph is recommended to monitor progress of labour in most low-and middle-income countries (LMICs), including India, although currently available evidence in this regard is insufficient. This study was conducted in the context of the highly successful JSY programme in three districts of Madhya Pradesh province. Participants 73 different level JSY programme facilities participated in the record review, 233 nurse midwives at these facilities participated in the vignette survey and a total of 11 doctors and midwives participated in the interviews. Results The partograph was used in 6% of the 1466 records reviewed. The staff obtained a median score of 1.08 (maximum of 10) at competence in plotting a partograph. Three themes emerged from the qualitative data: (1) partographs are used rarely and retrospectively; (2) training does not support correct use of the partograph; and (3) partographs can be useful but are not feasible. Conclusions Implementation fidelity of partograph use in the JSY programme is low. Successful implementation of the partograph can result in improved quality of care in the JSY programme only if potential moderators to its adherence, such as training, supervision, staff ‘buy in’ and practice environment are addressed so that staff find a conducive practice environment in which to use the partograph and women find it beneficial to present early in labour

What is the limit for photoautotrophic plankton growth rates?

© 2016 The Author. Knowing the potential maximum photoautotrophic growth rate for planktonic primary producers is fundamental to our understanding of trophic and biogeochemical processes, and of importance in applied phycology. When dayintegrated C-specific growth is considered over natural light:dark cycles, plausible RuBisCO activity (Kcat coupled with cellular RuBisCO content) caps growth to less than a few doubling per day. Prolonged periods of C-specific growth rates above ca. 1.3 d thus appear increasingly implausible. Discrepancies between RuBisCO-capped rates and reported microalgal-specific growth rates, including temperature-growth rate relationships, may be explained by transformational errors in growth rate determinations made by reference to cell counts or most often chlorophyll, or by extrapolations from short-Term measurements. Coupled studies of enzyme activity and day-on-day C-specific growth rates are required to provide definitive evidence of high growth rates. It seems likely, however, that selective pressure to evolve a RuBisCO with a high Kcat (with a likely concomitant increase in Km for CO2) would be low, as other factors such as light limitation (developing during biomass growth due to self-shading), nutrient limitations, CO2 depletion and pH elevation, would all rapidly depress realized specific growth rates