Major histocompatibility complex class I during pregnancy and at parturition in the horse placenta

"Materials and methods" folder contains details about RT-qPCR, conditions in which PCR and RT-PCR were performed, protocols for freezing tissues before staining and protocols for IHC-F, IF, IHC-P."Sequencing results" folder contains sequences of primer products for both RT-PCR and RT-qPCR."Data" folder contains data from PCR and RT-PCR (PCR and RT-PCR), relative expression of pooled MHC-I and B2M mRNA (RT-qPCR), relative expression of MHC-I protein (Western blots)."Scripts" folder contains scripts for mRNA and protein statistical analyses

Major histocompatibility complex class I during pregnancy and at parturition in the horse placenta

"Materials and methods" folder contains details about RT-qPCR, conditions in which PCR and RT-PCR were performed, protocols for freezing tissues before staining and protocols for IHC-F, IF, IHC-P."Sequencing results" folder contains sequences of primer products for both RT-PCR and RT-qPCR."Data" folder contains data from PCR and RT-PCR (PCR and RT-PCR), relative expression of pooled MHC-I and B2M mRNA (RT-qPCR), relative expression of MHC-I protein (Western blots)."Scripts" folder contains scripts for mRNA and protein statistical analyses.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Estimation of infection of internal parasites in horses from different type of farms

Studies were carried out in year 2014 during the pasture period (from April to October) in Warmia and Mazury Region. Fecal samples were taken from cold- and warmblood horses from individual and agrotouristic farms with the different housing, feeding and pasture- care practices. Total of 512 horses were examined (320 mares, 170 geldings and 22 stallions). In the group of 185 horses from individual farms, 119 animals (64.3%) were infected with gastro-intestinal parasites. Among the 372 horses from agrotouristic farms 169 (51.7%) were infected with parasites. Most of the animals expelled the eggs of Cyathostominae. In some individuals occurred eggs of Strongylus spp., Parascaris equorum, Strongyloides westeri and tapeworm of Anoplocephala. The number of infected horses from agrotouristic farms was lower than from individual farms, probably due to more regular deworming (usually 2 times a year) and bigger care paid to cleaning pastures