607 research outputs found
Erythropoietin production by fetal mouse liver cells in response to hypoxia and adenylate cyclase stimulation
This study was done to investigate aspects of control of extrarenal erythropoietin (Ep) production. To this end we studied the effects of three stimuli of renal Ep production in the adult, i.e. hypoxia, cobalt, and activation of adenylate cyclase on Ep generation by cultured fetal mouse liver cells. The fetal liver was taken as a model for extrarenal Ep production because this organ is considered the predominant site of extrarenal Ep production. We found that Ep production by the cells increased as the oxygen concentration was decreased in the incubation atmosphere from 20% to 1%. Cobalt (10(-4)-10(-5) M) had no effect on Ep production. Activation of adenylate cyclase by forskolin (10(-5) M) or isoproterenol (10(-5) M) greatly enhanced Ep production. These findings indicate that the Ep-stimulating effect of cobalt is specific for the kidney. However, oxygen depletion and activation of adenylate cyclase seem to be more general stimuli in Ep-producing cells. Furthermore we found that Ep production in hypoxia correlated with lactate formation in the cultured liver cells. This finding suggests that Ep production in fetal livers under hypoxic conditions parallels the shift from aerobic to anaerobic cellular energy metabolism
Hydrodynamical simulations of a compact source scenario for G2
The origin of the dense gas cloud G2 discovered in the Galactic Center
(Gillessen et al. 2012) is still a debated puzzle. G2 might be a diffuse cloud
or the result of an outflow from an invisible star embedded in it. We present
here detailed simulations of the evolution of winds on G2's orbit. We find that
the hydrodynamic interaction with the hot atmosphere present in the Galactic
Center and the extreme gravitational field of the supermassive black hole must
be taken in account when modeling such a source scenario. We find that the
hydrodynamic interaction with the hot atmosphere present in the Galactic Center
and the extreme gravitational field of the supermassive black hole must be
taken in account when modeling such a source scenario. We also find that in
this scenario most of the Br\gamma\ luminosity is expected to come from the
highly filamentary densest shocked wind material. G2's observational properties
can be used to constrain the properties of the outflow and our best model has a
mass outflow rate of Mdot,w=8.8 x 10^{-8} Msun/yr and a wind velocity of vw =
50 km/s. These values are compatible with those of a young TTauri star wind, as
already suggested by Scoville & Burkert (2013).Comment: 4 pages, 3 figures; Proceeding of the IAU 303: "The GC: Feeding and
Feedback in a Normal Galactic Nucleus" / September 30 - October 4, 2013,
Santa Fe, New Mexico (USA
3D AMR hydrosimulations of a compact source scenario for the Galactic Centre cloud G2
The nature of the gaseous and dusty cloud G2 in the Galactic Centre is still
under debate. We present three-dimensional hydrodynamical adaptive mesh
refinement (AMR) simulations of G2, modeled as an outflow from a "compact
source" moving on the observed orbit. The construction of mock
position-velocity (PV) diagrams enables a direct comparison with observations
and allow us to conclude that the observational properties of the gaseous
component of G2 could be matched by a massive () and slow ()
outflow, as observed for T Tauri stars. In order for this to be true, only the
material at larger () distances from the source must be
actually emitting, otherwise G2 would appear too compact compared to the
observed PV diagrams. On the other hand, the presence of a central dusty source
might be able to explain the compactness of G2's dust component. In the present
scenario, 5-10 years after pericentre the compact source should decouple from
the previously ejected material, due to the hydrodynamic interaction of the
latter with the surrounding hot and dense atmosphere. In this case, a new
outflow should form, ahead of the previous one, which would be the smoking gun
evidence for an outflow scenario.Comment: resubmitted to MNRAS after referee report, 16 pages, 11 figure
Infrared interferometry to spatially and spectrally resolve jets in X-ray binaries
Infrared interferometry is a new frontier for precision ground based
observing, with new instrumentation achieving milliarcsecond (mas) spatial
resolutions for faint sources, along with astrometry on the order of 10
microarcseconds. This technique has already led to breakthroughs in the
observations of the supermassive black hole at the Galactic centre and its
orbiting stars, AGN, and exo-planets, and can be employed for studying X-ray
binaries (XRBs), microquasars in particular. Beyond constraining the orbital
parameters of the system using the centroid wobble and spatially resolving jet
discrete ejections on mas scales, we also propose a novel method to discern
between the various components contributing to the infrared bands: accretion
disk, jets and companion star. We demonstrate that the GRAVITY instrument on
the Very Large Telescope Interferometer (VLTI) should be able to detect a
centroid shift in a number of sources, opening a new avenue of exploration for
the myriad of transients expected to be discovered in the coming decade of
radio all-sky surveys. We also present the first proof-of-concept GRAVITY
observation of a low-mass X-ray binary transient, MAXI J1820+070, to search for
extended jets on mas scales. We place the tightest constraints yet via direct
imaging on the size of the infrared emitting region of the compact jet in a
hard state XRB.Comment: 12 Pages, 3 figures, accepted for publication in MNRA
On the Ca2 + binding and conformational change in EF-hand domains: Experimental evidence of Ca2 +-saturated intermediates of N-domain of calmodulin
Double mutation of Q41L and K75I in the N-domain of calmodulin (N-Cam) stabilizes the closed form of N-Cam such that binding of Ca2 + in solution no longer triggers a conformational change to the open form, and its Ca2 + binding affinity decreases dramatically. To further investigate the solvation effects on the structure, Ca2 + binding affinity and conformational dynamics of this N-Cam double mutant in the Ca2 + saturated state, we solved its X-ray structure. Surprisingly, the structure revealed an open conformation of the domain which contradicts its closed conformation in solution. Here we provide evidence that crystallization conditions were responsible for this Ca2 +-saturated domain open conformation in the crystal. Importantly, we demonstrate that the presence of the crystallization co-precipitant and alcohols were able to induce a progressive opening of the closed form of this domain, in Ca2 + saturated state, in solution. However, in the Ca2 + depleted state, addition of alcohols was unable to induce any opening of this domain in solution. In addition, in the Ca2 + saturated state, the molecular dynamics simulations show that while N-Cam can populate the open and closed conformation, the N-Cam double mutant exclusively populates the closed conformation. Our results provide experimental evidence of intermediate conformations of Ca2 +-saturated N-Cam in solution. We propose that conformational change of Ca2 + sensor EF-hand domains depends on solvation energetics, Ca2 + binding to promote the full open form, Ca2 + depleted state conformational dynamics, and the chemical properties of the molecules nearby key residues such as those at positions 41 and 75 in N-Cam
The interferometric baselines and GRAVITY astrometric error budget
GRAVITY is a new generation beam combination instrument for the VLTI. Its
goal is to achieve microarsecond astrometric accuracy between objects separated
by a few arcsec. This accuracy on astrometric measurements is the most
important challenge of the instrument, and careful error budget have been
paramount during the technical design of the instrument. In this poster, we
will focus on baselines induced errors, which is part of a larger error budget.Comment: SPIE Meeting 2014 -- Montrea
The Post-Pericenter Evolution of the Galactic Center Source G2
In early 2014 the fast-moving near-infrared source G2 reached its closest
approach to the supermassive black hole Sgr A* in the Galactic Center. We
report on the evolution of the ionized gaseous component and the dusty
component of G2 immediately after this event, revealed by new observations
obtained in 2015 and 2016 with the SINFONI integral field spectrograph and the
NACO imager at the ESO VLT. The spatially resolved dynamics of the Br
line emission can be accounted for by the ballistic motion and tidal shearing
of a test-particle cloud that has followed a highly eccentric Keplerian orbit
around the black hole for the last 12 years. The non-detection of a drag force
or any strong hydrodynamic interaction with the hot gas in the inner accretion
zone limits the ambient density to less than a few 10 cm at the
distance of closest approach (1500 ), assuming G2 is a spherical cloud
moving through a stationary and homogeneous atmosphere. The dust continuum
emission is unresolved in L'-band, but stays consistent with the location of
the Br emission. The total luminosity of the Br and L' emission
has remained constant to within the measurement uncertainty. The nature and
origin of G2 are likely related to that of the precursor source G1, since their
orbital evolution is similar, though not identical. Both object are also likely
related to a trailing tail structure, which is continuously connected to G2
over a large range in position and radial velocity.Comment: 17 pages, 12 figures; accepted for publication in Ap
GRAVITY: the Calibration Unit
We present in this paper the design and characterisation of a new sub-system
of the VLTI 2nd generation instrument GRAVITY: the Calibration Unit. The
Calibration Unit provides all functions to test and calibrate the beam combiner
instrument: it creates two artificial stars on four beams, and dispose of four
delay lines with an internal metrology. It also includes artificial stars for
the tip-tilt and pupil guiding systems, as well as four metrology pick-up
diodes, for tests and calibration of the corresponding sub-systems. The
calibration unit also hosts the reference targets to align GRAVITY to the VLTI,
and the safety shutters to avoid the metrology light to propagate in the
VLTI-lab. We present the results of the characterisation and validtion of these
differrent sub-units.Comment: 12 pages, 11 figures. Proceeding of SPIE 9146 "Optical and Infrared
Interferometry IV
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