21 research outputs found
Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development
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Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle
Skeletal muscle regeneration is mainly enabled by a population of adult stem cells known as satellite cells. Satellite cells have been shown to be indispensable for adult skeletal muscle repair and regeneration. In the last two decades, other stem/progenitor cell populations resident in the skeletal muscle interstitium have been identified as "collaborators" of satellite cells during regeneration. They also appear to have a key role in replacing skeletal muscle with adipose, fibrous, or bone tissue in pathological conditions. Here, we review the role and known functions of these different interstitial skeletal muscle cell types and discuss their role in skeletal muscle tissue homeostasis, regeneration, and disease, including their therapeutic potential for cell transplantation protocols
Transcriptome analysis of cattle muscle identifies potential markers for skeletal muscle growth rate and major cell types
Parental and sexual conflicts over the Peg3 imprinted domain
In the current study, the imprinting control region of the mouse Peg3 domain was deleted to test its functional impact on animal growth and survival. The paternal transmission of the deletion resulted in complete abolition of the transcription of two paternally expressed genes, Peg3 and Usp29, causing the reduced body weight of the pups. In contrast, the maternal transmission resulted in the unexpected transcriptional up-regulation of the remaining paternal allele of both Peg3 and Usp29, causing the increased body weight and survival rates. Thus, the imprinted maternal allele of the ICR may be a suppressor antagonistic to the active paternal allele of the ICR, suggesting a potential intralocus allelic conflict. The opposite outcomes between the two transmissions also justify the functional compromise that the maternal allele has become epigenetically repressed rather than genetically deleted during mammalian evolution. The mice homozygous for the deletion develop normally but with a skewed sex ratio, one male per litter, revealing its sex-biased effect. Overall, the Peg3 locus may have evolved to an imprinted domain to cope with both parental and sexual conflicts driven by its growth-stimulating paternal versus growth-suppressing maternal alleles
A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells
Influence of the built environment of Nanjing’s Urban Community on the leisure physical activity of the elderly: an empirical study
Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression
Resistance wheel exercise from mid-life has minimal effect on sciatic nerves from old mice in which sarcopenia was prevented
The ability of resistance exercise, initiated from mid-life, to prevent age-related changes in old sciatic nerves, was investigated in male and female C57BL/6J mice. Aging is associated with cellular changes in old sciatic nerves and also loss of skeletal muscle mass and function (sarcopenia). Mature adult mice aged 15Â months (M) were subjected to increasing voluntary resistance wheel exercise (RWE) over a period of 8Â M until 23Â M of age. This prevented sarcopenia in the old 23Â M aged male and female mice. Nerves of control sedentary (SED) males at 3, 15 and 23Â M of age, showed a decrease in the myelinated axon numbers at 15 and 23Â M, a decreased g-ratio and a significantly increased proportion of myelinated nerves containing electron-dense aggregates at 23Â M. Myelinated axon and nerve diameter, and axonal area, were increased at 15Â M compared with 3 and 23Â M. Exercise increased myelinated nerve profiles containing aggregates at 23Â M. S100 protein, detected with immunoblotting was increased in sciatic nerves of 23Â M old SED females, but not males, compared with 15Â M, with no effect of exercise. Other neuronal proteins showed no significant alterations with age, gender or exercise. Overall the RWE had no cellular impact on the aging nerves, apart from an increased number of old nerves containing aggregates. Thus the relationship between cellular changes in aging nerves, and their sustained capacity for stimulation of old skeletal muscles to help maintain healthy muscle mass in response to exercise remains unclear