88 research outputs found
Grape seed and linseed, alone and in combination, enhance unsaturated fatty acids in the milk of Sarda dairy sheep
Comparison of fatty acid profile in lamb meat and baby food based on lamb meat
The aim of this study was to compare the fatty acid (FA) profile of fresh lamb meat
with those of baby foods based on lamb meat. For this purpose, samples of commercial homogenized
(HO) and lyophilized (LIO) baby food based on lamb meat and fresh lamb meat (FM) were analyzed
for their FA composition. All fatty acids, except for oleic acid and conjugated linoleic acid (CLA), differed
among the three baby products tested. The sum of omega-6 FA and the ratio omega 6/omega 3
FA were lower in FM and LIO meat compared to HO samples. The content of total PUFA n-3 was the highest in FM, because of its highest content of C18:3 n3, EPA, DPA and DHA compared to LIO and
HO baby food. The content of arachidonic acid was more than 6-fold higher in FM compared to LIO and
HO. This study evidenced the possibility of enhancing the FA profile of commercial baby food based on lamb meat by using meat with healthier FA profile
Infusion of casein hydrolizates into the mammary gland simulates the omission of one daily milking in goats
Suppression of one daily milking at weekends, even though socially desirable, may
reduce milk yield. These losses have been attributed to a short-term mechanism: the filling of the cistern and
ductal-alveolar system with milk which contains a peptide called feedback inhibitor of lactation (FIL) (Wilde
and Peaker, 1990). The FIL probably reduces the synthesis and secretion of mammary cells by blocking the
potassium channel of the apical membrane (Silanikove et al., 2000). Shamay et al. (2002) hypothesized that
the FIL can be identified with the AA sequence 1-28, derived from the breakdown of β-casein by plasmin (PL).
The aim of this work was to verify if the infusion of casein hydrolizates (CNH) into the mammary gland simulates
the omission of one milking for two consecutive days
Machine milking management and milk nitrogen fractions in primiparous ewes
Little work has been carried out on management techniques to reduce stress of first machine
milking in ewes. The aim of this work was to evaluate the effect of milking parlour training before weaning on sheep
milk yield and milk protein fractions. One week before weaning, an experimental group (EG) was introduced into
the machine milking parlour and hand-milked once a day, to adapt them to the new environment and noise of the
milking machine, while a control group (CG) was hand-milked once a day in a traditional fold parlour until weaning.
After weaning, both groups were machine milked, separately, twice a day in the milking parlour. Milk yield and
nitrogen fractions were recorded for the first 10 days of machine milking. Differences between samplings within
group were observed for all parameters considered. Only on the first day of machine milking, differences between
groups occurred for total nitrogen, total protein and casein. Higher content of nitrogen fractions in the milk of EG
was due to its lower milk production. In fact, daily production of nitrogen fractions was higher in CG than in EG.
High variability for most parameters was observed during the first 5 days of machine milking in both groups. A
week of training to machine milking parlour was not enough to reduce the negative effects of weaning and machine
milking stresses on primiparous ewes. (interaction?
Maternal and fetal fatty acid composition in ovine muscle tissues
In species characterized by a cotyledonary placenta, as sheep, the relative contribution of maternally derived and
placenta synthesized fatty acids is not fully understood. For this reason, the FA composition of mother muscle and
the deposition of FA in the fetal muscle were studied by gas-chromatography. Five pregnant Sarda ewes were
slaughtered at approximately 145 days of pregnancy. Semitendinosus, semimembranosus and femoral biceps muscles
were immediately removed from ewes and fetuses. Data were analyzed by a paired t-test, to detect differences
in FA composition between fetus and mother tissues. Results showed that FA profile of fat muscle differed markedly
between fetus and mother. The intramuscular fat content were 6.38% and 11.79% on DM basis in fetus and mother
muscle, respectively. Linoleic (LA; 18:2n-6), and linolenic (ALA; 18:3n-3) acid were found at smaller concentrations
in fetus (0.77 and 0.01 mg/100 mg total FA for LA and ALA, respectively) than in maternal muscle (5.55 and
1.04 for LA and ALA, respectively). On the contrary, a higher proportion of their long-chain polyunsaturated
metabolites, like arachidonic (AA; 20:4n-6) and docoexadienoic (DHA; 22:6n-3) acid in fetus compared to mother tissue
(AA, 2.32 vs 1.30; DHA, 0.83 vs 0.09 mg/100 mg total FA) indicates a preferential fetal accumulation of those
FA which are important for fetuses growth and central nervous system development. The c9,t11 Conjugated linoleic
acid (CLA) isomer concentration in mother tissue was 0.72 mg/100 mg FA. It was found also in fetus muscle (0.11
mg/100 mg total FA) suggesting a transplacental fatty acid passage to fetal tissues or a desaturation activity on
vaccenic acid (VA; 18:1 t11) in the placental or in the fetal tissue. Surprisingly, the c9,t11-CLA was not the most
abundant CLA isomer found in fetus fat. Others CLA isomers, separated but not identified, were found in concentration
of 0.17, 0.15 and 0.53 mg/100 mg of FA. Concentrations of these CLA isomers were lower in mother tissue
(0.12, 0.05 and 0.10 mg/100 mg of FA). The concentration of VA was 0.46 and 0.90 in fetus and mother muscle,
respectively. Correlation analysis between FA profile of mothers and fetuses evidenced a close relationship only for
odd-numbered long-chain fatty (r = -0.72 for C15:0 and r = -0.88 for C17:0). The results suggested a different FA
metabolism in the muscle tissue of mother and fetus in dairy sheep
Prenatal exposure to different diets influences programming of glucose and insulin metabolism in dairy ewes.
ABSTRACT Nutrition in fetal and postnatal life can influence the development of several biological systems, with permanent effects in adult life. The aim of this work was to investigate in dairy sheep whether diets rich in starch or fiber during intrauterine life (75 d before lambing) and postnatal life (from weaning to first pregnancy; growth phase) program glucose and insulin metabolism in the female offspring during their first pregnancy. Starting from intrauterine life, 20 nulliparous Sarda ewes were exposed to 4 dietary regimens (n = 5 per group) based on different dietary carbohydrates during their intrauterine life and their subsequent growth phase: (1) the fiber (FI) diet during both intrauterine and growth life, (2) the starch (ST) diet during both intrauterine and growth life, (3) the FI diet in intrauterine life followed by the ST diet in the growth phase, and (4) the ST diet in intrauterine life followed by the FI diet in the growth phase. After the end of the growth phase, all growing ewes were fed the same diet and naturally mated. When ewes were pregnant, on average at 124 ± 2 d of gestation they were challenged with an intravenous glucose tolerance test, and peripheral concentrations of glucose and insulin were determined. Basal insulin concentrations were higher in ewes exposed to the ST diet (0.97 μg/L) than in ewes exposed to the FI diet (0.52 μg/L) in intrauterine life. After glucose infusion, glucose and insulin concentrations were not affected by intrauterine diet. Insulin resistance, determined by the homeostasis model assessment, was affected by the intrauterine × growth phases interaction. Insulin sensitivity, assessed by the quantitative insulin check index, was lower in ewes exposed to the ST diet than in those exposed to the FI diet in intrauterine life (ST = 0.28; FI = 0.30). Diet in growth life had no effect on glucose and insulin metabolism. In conclusion, starchy diets offered during intrauterine life but not during postnatal life increased basal insulin level and lowered insulin sensitivity during the first pregnancy. Nutritional strategies of metabolic programming should consider that exposure to starchy diets in late fetal life might favor the programming of dietary nutrient partitioning toward organs with high requirements, such as the gravid uterus or the mammary gland
Mapping quantitative trait loci (QTL) in sheep. II. Meta-assembly and identification of novel QTL for milk production traits in sheep
An (Awassi × Merino) × Merino backcross family of 172 ewes was used to map quantitative trait loci (QTL) for different milk production traits on a framework map of 200 loci across all autosomes. From five previously proposed mathematical models describing lactation curves, the Wood model was considered the most appropriate due to its simplicity and its ability to determine ovine lactation curve characteristics. Derived milk traits for milk, fat, protein and lactose yield, as well as percentage composition and somatic cell score were used for single and two-QTL approaches using maximum likelihood estimation and regression analysis. A total of 15 significant (P < 0.01) and additional 25 suggestive (P < 0.05) QTL were detected across both single QTL methods and all traits. In preparation of a meta-analysis, all QTL results were compared with a meta-assembly of QTL for milk production traits in dairy ewes from various public domain sources and can be found on the ReproGen ovine gbrowser http://crcidp.vetsci.usyd.edu.au/cgi-bin/gbrowse/oaries_genome/. Many of the QTL for milk production traits have been reported on chromosomes 1, 3, 6, 16 and 20. Those on chromosomes 3 and 20 are in strong agreement with the results reported here. In addition, novel QTL were found on chromosomes 7, 8, 9, 14, 22 and 24. In a cross-species comparison, we extended the meta-assembly by comparing QTL regions of sheep and cattle, which provided strong evidence for synteny conservation of QTL regions for milk, fat, protein and somatic cell score data between cattle and sheep
Produção e composição do leite de ovelhas Santa Inês e mestiças Lacaune e Santa Inês e desenvolvimento de seus cordeiros
Estimates of genomic heritability and genome-wide association study for fatty acids profile in Santa Inês sheep
Background: Despite the health concerns and nutritional importance of fatty acids, there is a relative paucity of studies in the literature that report genetic or genomic parameters, especially in the case of sheep populations. To investigate the genetic architecture of fatty acid composition of sheep, we conducted genome-wide association studies (GWAS) and estimated genomic heritabilities for fatty acid profile in Longissimus dorsi muscle of 216 male sheep.
Results: Genomic heritability estimates for fatty acid content ranged from 0.25 to 0.46, indicating that substantial genetic variation exists for the evaluated traits. Therefore, it is possible to alter fatty acid profiles through selection. Twenty-seven genomic regions of 10 adjacent SNPs associated with fatty acids composition were identified on chromosomes 1, 2, 3, 5, 8, 12, 14, 15, 16, 17, and 18, each explaining ≥0.30% of the additive genetic variance. Twenty-three genes supporting the understanding of genetic mechanisms of fat composition in sheep were identified in these regions, such as DGAT2, TRHDE, TPH2, ME1, C6, C7, UBE3D, PARP14, and MRPS30.
Conclusions: Estimates of genomic heritabilities and elucidating important genomic regions can contribute to a better understanding of the genetic control of fatty acid deposition and improve the selection strategies to enhance meat quality and health attributes
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