Increased levels of RNA oxidation enhance the reversion frequency in aging pro-apoptotic yeast mutants

Despite recent advances in understanding the complexity of RNA processes, regulation of the metabolism of oxidized cellular RNAs and the mechanisms through which oxidized ribonucleotides affect mRNA translation, and consequently cell viability, are not well characterized. We show here that the level of oxidized RNAs is markedly increased in a yeast decapping Kllsm4Δ1 mutant, which accumulates mRNAs, ages much faster that the wild type strain and undergoes regulated-cell-death. We also found that in Kllsm4Δ1 cells the mutation rate increases during chronological life span indicating that the capacity to han- dle oxidized RNAs in yeast declines with aging. Lowering intracellular ROS levels by antioxidants recovers the wild- type phenotype of mutant cells, including reduced amount of oxidized RNAs and lower mutation rate. Since mRNA oxidation was reported to occur in different neurodegen- erative diseases, decapping-deficient cells may represent a useful tool for deciphering molecular mechanisms of cell response to such conditions, providing new insights into RNA modification-based pathogenesis

Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumine in Kluyveromyces lactis

KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity which is specifically induced by ethanol. The promoter of this gene was used for the expression of heterologous proteins in K. lactis, a very promising organism which can be used as an alternative host to Saccharomyces cerevisiae due to its good secretory properties. In this paper we report the ethanol-driven expression in K. lactis of the bacterial beta-glucuronidase and of the human serum albumin (HSA) genes under the control of the KlADH4 promoter. In particular, we studied the extracellular production of recombinant HSA (rHSA) with integrative and replicative vectors and obtained a significant increase in the amount of the protein with multicopy vectors, showing that no limitation of KlADH4 trans-acting factors occurred in the cells. By deletion analysis of the promoter, we identified an element (UASE) which is sufficient for the induction of KlADH4 by ethanol and, when inserted in the respective promoters, allows ethanol-dependent activation of other yeast genes, such as PGK and LAC4. We also analyzed the effect of medium composition on cell growth and protein secretion. A clear improvement in the production of the recombinant protein was achieved by shifting from batch cultures (0.3 g/liter) to fed-batch cultures (1 g/liter) with ethanol as the preferred carbon source

Low mass dimuons within a hybrid approach

We analyse dilepton emission from hot and dense hadronic matter using a hybrid approach based on the Ultrarelativistic Quantum Molecular Dynamics (UrQMD) transport model with an intermediate hydrodynamic stage for the description of heavy-ion collisions at relativistic energies. Focusing on the enhancement with respect to the contribution from long-lived hadron decays after freeze-out observed at the SPS in the low mass region of the dilepton spectra (often referred to as "the excess"), the relative importance of the emission from the equilibrium and the non-equilibrium stages is discussed.Comment: Proceedings of Hot Quarks 2010, 21-26 June 2010 Las Londe Les Maures; v2: Corrected typos and added a commen

The Two-Screen Measurement Setup to Indirectly Measure Proton Beam Self-Modulation in AWAKE

The goal of the first phase of the AWAKE \cite{AWAKE1,AWAKE2} experiment at CERN is to measure the self-modulation \cite{SMI} of the $\sigma_z = 12\,\rm{cm}$ long SPS proton bunch into microbunches after traversing $10\,\rm{m}$ of plasma with a plasma density of $n_{pe}=7\times10^{14}\,\rm{electrons/cm}^3$. The two screen measurement setup \cite{Turner2016} is a proton beam diagnostic that can indirectly prove the successful development of the self-modulation of the proton beam by imaging protons that got defocused by the transverse plasma wakefields after passing through the plasma, at two locations downstream the end of the plasma. This article describes the design and realization of the two screen measurement setup integrated in the AWAKE experiment. We discuss the performance and background response of the system based on measurements performed with an unmodulated Gaussian SPS proton bunch during the AWAKE beam commissioning in September and October 2016. We show that the system is fully commissioned and adapted to eventually image the full profile of a self-modulated SPS proton bunch in a single shot measurement during the first phase of the AWAKE experiment.Comment: 5 pages 8 figure

A Proposal for an Environmental Decision Support System at the Regional Level: Concepts, Support Methodology, Tools and their Terminology

One of the goals of IIASA's research activities in the area of environmental quality modeling is the integration of data and models in a unified framework to assist decision makers with the management of complex environmental systems. Building on IIASA's work undertaken within the WELMM (Water, Energy, Land, Materials and Manpower) project of the former Resources and Environment Area and the work on Decision Support Systems of the former Management and Technology Area, a conceptual framework for an environmental decision support system (EDSS) has been developed and is presented in this paper. The proposed EDSS has been developed with the interest and the financial support of the CSI, the Center for Information Systems of the Regional Government of Piemonte, Italy. The main issue addressed by this paper is to devise a system assisting decision makers in tackling environmental problems at the regional level. These decisions are typically characterized by a combination of both structured (formalizable, described in a quantitative model) and unstructured elements (incomplete information, undefined cause-effect relationships, influence of political objectives, public perception, consideration of aesthetics, etc.). The proposed EDSS enables the user to use models and data, of relevance to a particular task, which are embedded in the EDSS in the form of a process information system. The specific feature of this process information system is that it contains processes of anthropogenic nature (the socio-economic activities being the cause of environmental impacts like power plants, industrial production units, etc.) as well as natural processes determining the spatial/temporal distribution and the extent of environmental quality changes (like the dispersion and deposition of air pollutants and their effect on human population, vegetation and wildlife). The system ensures that the data and models, which have been developed in the context of specific EDSS applications are documented right from the outset and become thus equally available for further use. This becomes especially important in view of the long-term effort to be put into the development of data and models dealing with the large number of environmental problems that governments, industry and academic institutions are confronted with at the regional level

Late Quaternary monogenetic volcanoes along Río Salado, Sothwest Mendoza Province, Argentina

On the eastem flank of the Andes, to the north of Río Salado in southwest Mendoza Province (35º07'S-35º10'S), there are 4 monogenetic cones with blocky lava flows. A western group of small volcanoes, Hoyada, Lagunita and Loma Negra, with a total volume of -0.2 km3, are composed of amphibole-bearing basaltic andesite, and the eastem, more voluminous Hoyo Colorado volcano, with 0.44 km3 is composed of olivine (+ oxidised amphibole) basaltic andesite. Although data indicate they were emitted through successive, strombolian eruptions, they are overall coeval and the youngest Late Pleistocene volcanoes located in an "extra-Andean" setting, -70 km east of the main volcanic front. The magmas of the westem group of monogenetic cones show petrographic and geochemical characteristics that support processes of crustal interaction during ascent. In contrast, the magmas of the Hoyo Colorado volcano had a more direct ascent. Structural characteristics of the basement rocks to the volcanoes and the current seismotectonic activity of the Andes at this latitude indicate that the monogenetic cones of Río Salado were emplaced in a dominantly compressive tectonic regime

False claims about false memory research

Pezdek and Lam [Pezdek, K. & Lam, S. (2007). What research paradigms have cognitive psychologists used to study “False memory,” and what are the implications of these choices? Consciousness and Cognition] claim that the majority of research into false memories has been misguided. Specifically, they charge that false memory scientists have been (1) misusing the term “false memory,” (2) relying on the wrong methodologies to study false memories, and (3) misapplying false memory research to real world situations. We review each of these claims and highlight the problems with them. We conclude that several types of false memory research have advanced our knowledge of autobiographical and recovered memories, and that future research will continue to make significant contributions to how we understand memory and memory errors

Group theory analysis of electrons and phonons in N-layer graphene systems

In this work we study the symmetry properties of electrons and phonons in graphene systems as function of the number of layers. We derive the selection rules for the electron-radiation and for the electron-phonon interactions at all points in the Brillouin zone. By considering these selection rules, we address the double resonance Raman scattering process. The monolayer and bilayer graphene in the presence of an applied electric field are also discussed.Comment: 8 pages, 6 figure