Cocaine- and amphetamine-regulated transcript (CART) peptide in the nerve fibres of the porcine gallbladder wall under physiological conditions and after Salmonella Enteritidis lipopolysaccharides administration

Background: Cocaine- and amphetamine-regulated transcript (CART) peptide is the substance distributed in various parts of the nervous system. The majority of previous studies described this substance in the brain, where it takes part in regulatory processes connected with the food intake. CART is also present in the peripheral nervous system, especially in the enteric neurons and nerves located in the wall of the stomach and intestine, but many aspects of distribution and functions of this peptide in the digestive organs remain unknown. The aim of the present study was to investigate the changes of CART-positive nerves in the porcine gallbladder after administration of low-dose Salmonella Enteritidis lipopolysaccharide (LPS) using the single immunofluorescence technique. Materials and methods: Seven days after the injection of 5 μg/kg b.w. LPS S. Enteritidis the gallbladders were collected. CART-positive nerves were studied with standard single immunofluorescence method and counted per observation field (0.1 m2). Results: In control animals the average number of CART-positive nerves per observation field (0.1 mm2) amounted to 5.38 ± 0.32, 11.11 ± 1.56 and 2.97 ± ± 0.24 in gallbladder neck, body and fundus, respectively. LPS administration caused the increase in the number of CART-positive fibres in all parts of gallbladder, and these values amounted to 12.74 ± 0.51, 19.75 ± 0.19 and 5.1 ± 0.05 in the gallbladder neck, body and fundus, respectively. Conclusions: The obtained results suggest that CART is involved in the neuronal regulatory processes in the porcine gallbladder under physiological conditions, but also during pathological processes, but exact functions of this peptide in this organ remain unexplained and require the further investigation

Геология Эльмусской площади и проект поисковых работ на рудное золото в пределах участка Талпус (Республика Карелия)

Выпускная квалификационная работа 126 с., 5 рис., 43 табл., 18 источников, 4 прил. Ключевые слова: рудопроявление золота, участок Талпус, Эльмусская площадь, поисковые работы, геохимическая модель, прогнозные ресурсы. Объектом исследования является (ются) Рудопроявление Талпус. Цель работы – Обоснование постановки поисковых работ и разработка методики поисковых работ. В процессе исследования проводились изучение геохимической характеристики рудопроявления Талпус, разработка методики поисковых работ и экономическая оценка. В результате исследования дано обоснование поисковых работ, построена геохимическая модель рудопроявления, подсчитаны экономические затраты проектируемых работ. Основные конструктивные, технологические и технико-эксплуатационные характеристики: проектируемые работыFinal qualifying work 126 p., 5 fig., 43 tab., 18 sources, Appendix 4. Keywords: ore occurrence of gold, Talpus site Elmusskaya area search works, geochemical model, inferred resources. The object of this study is (are) Levo Talpus. Objective - Rationale statement prospecting and development of methods of prospecting. The study carried out the study of geochemical characteristics of the ore Talpus, development of methods of prospecting and economic evaluation. The study substantiates prospecting, geochemical model built ore, calculated the economic costs projected works. The basic constructive, technological and technical and operational characteristics: the projected works are consistent with the staging of exploration and meet the basic requirements of setting prospecting Degree

Construction of an instant structured illumination microscope

A challenge in biological imaging is to capture high-resolution images at fast frame rates in live cells. The “instant structured illumination microscope” (iSIM) is a system designed for this purpose. Similarly to standard structured illumination microscopy (SIM), an iSIM provides a twofold improvement over widefield microscopy, in x, y and z, but also allows much faster image acquisition, with real-time display of super-resolution images. The assembly of an iSIM is reasonably complex, involving the combination and alignment of many optical components, including three micro-optics arrays (two lenslet arrays and an array of pinholes, all with a pitch of 222 μm) and a double-sided scanning mirror. In addition, a number of electronic components must be correctly controlled. Construction of the system is therefore not trivial, but is highly desirable, particularly for live-cell imaging. We report, and provide instructions for, the construction of an iSIM, including minor modifications to a previous design in both hardware and software. The final instrument allows us to rapidly acquire fluorescence images at rates faster than 100 fps, with approximately twofold improvement in resolution in both x-y and z; sub-diffractive biological features have an apparent size (full width at half maximum) of 145 nm (lateral) and 320 nm (axial), using a 1.49 NA objective and 488 nm excitation

Inhibition of the \u3cem\u3edapE\u3c/em\u3e-Encoded \u3cem\u3eN\u3c/em\u3e-Succinyl- ʟ, ʟ-diaminopimelic Acid Desuccinylase from \u3cem\u3eNeisseria meningitidis\u3c/em\u3e by ʟ-Captopril

Binding of the competitive inhibitor ʟ-captopril to the dapE-encoded N-succinyl-ʟ, ʟ-diaminopimelic acid desuccinylase from Neisseria meningitidis (NmDapE) was examined by kinetic, spectroscopic, and crystallographic methods. ʟ-Captopril, an angiotensin-converting enzyme (ACE) inhibitor, was previously shown to be a potent inhibitor of the DapE from Haemophilus influenzae (HiDapE) with an IC50 of 3.3 μM and a measured Ki of 1.8 μM and displayed a dose-responsive antibiotic activity toward Escherichia coli. ʟ-Captopril is also a competitive inhibitor of NmDapE with a Ki of 2.8 μM. To examine the nature of the interaction of ʟ-captopril with the dinuclear active site of DapE, we have obtained electron paramagnetic resonance (EPR) and magnetic circular dichroism (MCD) data for the enzymatically hyperactive Co(II)-substituted forms of both HiDapE and NmDapE. EPR and MCD data indicate that the two Co(II) ions in DapE are antiferromagnetically coupled, yielding an S = 0 ground state, and suggest a thiolate bridge between the two metal ions. Verification of a thiolate-bridged dinuclear complex was obtained by determining the three-dimensional X-ray crystal structure of NmDapE in complex with ʟ-captopril at 1.8 Å resolution. Combination of these data provides new insights into binding of ʟ-captopril to the active site of DapE enzymes as well as important inhibitor–active site residue interaction’s. Such information is critical for the design of new, potent inhibitors of DapE enzymes

Маркшейдерский учет добычи угля

В монографии приведены результаты исследования точности маркшейдерского учета добычи угля, позволившие установить влияние геомеханического состояния угольного пласта по линии очистного забоя, геометрических параметров тектонических нарушений и искривленности линии очистного забоя на точность определения объемов добычи. Результаты исследований позволяют усовершенствовать методику маркшейдерских замеров выработанного пространства, и обеспечивают повышение точности и оперативности учета добычи

Проектирование информационной системы для валютных операций в банке

Выпускная квалификационная работа выполнена на 97 с., 18 рисунков, 36 табл., 26 источников. Объектом исследования являются валютные операции в банке, расчет доходов и расходов банка, гибкий курс обмена и процесс переоценки валюты в банке. Цель работы – разработка информационной системы для реализации валютных операций в банке. В процессе исследования проводился анализ предметной области – валютные операции банка и правила их учета в валютной бухгалтерии. В результате исследования была спроектирована банковская информационная система 슫Валютные операции슻.Final qualifying work is done on 97 p., 18 figures, 36 tables., 26 sources. Object of research are foreign exchange transactions in the Bank, calculate the income and expenses of the Bank, flexible exchange rate and the process of currency revaluation in Bank. The work purpose – development of information system for realization of currency operations at the Bank. In the process of investigation the analysis of the subject area – foreign exchange operations of the Bank and their accounting in foreign currency accounting. The study was designed by the banking information system "foreign Exchange transactions"