Construction and validation of a games-based intelligence assessment in Minecraft

Video games are a promising tool for the psychometric assessment of cognitive abilities. They can present novel task types and answer formats, they can record process data, and they can be highly motivating for test takers. This paper introduces the first game-based intelligence assessment implemented in Minecraft, an exceptionally popular video game with 176m copies sold. A matrix-based pattern completion task (PC), a mental rotation task (MR) and a spatial construction task (SC) were implemented in the three-dimensional, immersive environment of the game. PC was intended as a measure of inductive reasoning, whereas MR and SC were measures of spatial ability. We tested 129 children aged 10-12 years old on the Minecraft-based tests as well as equivalent pen-and-paper tests. All three scales fit the Rasch model and were moderately reliable. Factorial validity was good with regard to the distinction between PC and SC, but no distinct factor was found for MR. Convergent validity was good as abilities measured with Minecraft and conventional tests were highly correlated at the latent level (r = .72). Subtest-level correlations were in the moderate range. Furthermore, we found that behavioral log-data collected from the game environment was highly predictive of performance in the Minecraft test and, to a lesser extent, also predicted scores in conventional tests. We identify a number of behavioral features associated with spatial reasoning ability, demonstrating the utility of analyzing granular behavioral data in addition to traditional response formats. Overall, our findings indicate that Minecraft is a suitable platform for game-based intelligence assessment and encourage future work aiming to explore game-based problem solving tasks that would not be feasible on paper or in conventional computer-based tests

Localization of three host-protective oncospheral antigens of Taenia ovis

Immunohistochemistry, confocal immunofluorescence and immunogold labelling were used to determine the localisation of the host-protective antigens To16, To18 and To45W in Taenia ovis oncospheres. During maturation of the adult tapeworm the antigens were initially seen as diffuse staining in the developing oncospheres but in mature oncospheres four distinct cells stained positively for the antigens. Confocal fluorescence microscopy using different fluorophores revealed that each of the antigens co-localises within the same cells in the oncosphere. No surface localisation was seen in non-activated or recently activated parasites. Immunogold labelling of non-activated oncosphere sections viewed in transmission electron microscopy revealed labelling of bilateral cells, however the identities of these cells was unclear due to deficiencies in the current level of understanding of oncosphere ultrastructure. Localisation of all the antigens changed dramatically after oncospheres were activated in vitro with each of the antigens being dispersed more generally throughout the parasite parenchyma. During development of the parasites in in vitro culture, surface localisation of the proteins was seen in parasites after 3 or more days in culture. All three antigens were found to be completely absent in parasites by 15. days of culture

Antibody responses and epitope specificities to the Taenia solium cysticercosis vaccines TSOL18 and TSOL45-1A

Taenia solium is a cestode parasite that causes cysticercosis in humans and pigs. This study examined the antibody responses in pigs immunized with the TSOL18 and TSOL45-1A recombinant vaccines against T. solium cysticercosis. Immunization with these proteins induced specific, complement-fixing antibodies against the recombinant antigens that are believed to be associated with vaccine-induced protection against T. solium infection. Sera from immunized pigs were used to define the linear B-cell epitopes of TSOL18 and TSOL45-1A. Prominent reactivity was revealed to one linear epitope on TSOL18 and two linear epitopes on TSOL45-1A. These, and oncosphere antigens from other taeniid cestodes, contain a protein sequence motif suggesting that they may show a tertiary structure similar to the fibronectin type III domain (FnIII). Comparison of the location of linear antigenic epitopes in TSOL18 and TSOL45-1A within the proposed FnIII structure to those within related cestode vaccine antigens reveals conservation in the positioning of the epitopes between oncosphere antigens from different taeniid species

The Bipolarity of Attitudes: Unfolding the Implications of Ambivalence

Recently, some attitude researchers have argued that the traditional bipolar model of attitudes should be replaced, claiming that a bivariate model is superior in several ways, foremost of which is its ability to account for ambivalent attitudes. This study argues that ambivalence is not at odds with bipolarity per se, but rather the conventional view of bipolarity, and that the psychometric evidence supporting a bivariate interpretation has been flawed. To demonstrate this, a scale developed out of the bivariate approach was examined using a unidimensional unfolding item response theory model: general hyperbolic cosine model for polytomous responses. The results were consistent with a bipolar interpretation, providing support for the argument that ambivalent evaluations are the correct middle-point of a bipolar evaluative dimension. Thus, it is argued that attitudinal ambivalence does not necessitate moving beyond bipolarity, but rather, moving beyond the conventional conceptualization and assessment of attitudes

Elimination of Taenia solium transmission to pigs in a field trial of the TSOL18 vaccine in Cameroon

A pilot field trial of the TSOL18 vaccine was undertaken in Cameroon. Two hundred and forty, 2–3 month-old piglets were distributed to 114 individual households in pairs. Vaccinated animals received three immunisations with 200 μg TSOL18 plus 5 mg Quil A and 30 mg/kg oxfendazole at the time of the second immunisation. Necropsies were undertaken when the pigs were approximately 12 months of age. Viable Taenia solium cysticerci were identified in 20 control pigs (prevalence 19.6%); no cysticerci were found in any of the vaccinated animals (P < 0.0001). Combined application of TSOL18 vaccination and a single oxfendazole treatment in pigs may be a relatively simple and sustainable procedure that has the potential to control T. solium transmission in endemic areas and, indirectly, reduce the number of new cases of neurocysticercosis in humans

Efficacy of the EG95 hydatid vaccine in a macropodid host, the tammar wallaby

In Australia, macropodids are common intermediate hosts for the cestode Echinococcus granulosus, and sylvatic transmission is maintained via wild dogs. The parasite causes mortality in a number of macropodid species and the sylvatic cycle provides a source of infection to domestic livestock and humans. We determined the efficacy of the hydatid vaccine, EG95 in the tammar wallaby, Macropus eugenii, challenging either 1 or 9 months post-vaccination. EG95 provides similar protection to that seen in sheep (96-100%). Control tammars were significantly more likely to become infected (odds ratio 29.44; CI 4.13, 209.97; P=0.001) and to develop more cysts (count ratio 26.69; CI 5.83, 122.19;