21 research outputs found
Animal models for aberrations of gonadotropin action
During the last two decades a large number of genetically modified mouse lines with altered gonadotropin action have been generated. These mouse lines fall into three categories: the lack-of-function mice, gain-of-function mice, and the mice generated by breeding the abovementioned lines with other disease model lines. The mouse strains lacking gonadotropin action have elucidated the necessity of the pituitary hormones in pubertal development and function of gonads, and revealed the processes from the original genetic defect to the pathological phenotype such as hypo- or hypergonadotropic hypogonadism. Conversely, the strains of the second group depict consequences of chronic gonadotropin action. The lines vary from those expressing constitutively active receptors and those secreting follicle-stimulating hormone (FSH) with slowly increasing amounts to those producing human choriogonadotropin (hCG), amount of which corresponds to 2000-fold luteinizing hormone (LH)/hCG biological activity. Accordingly, the phenotypes diverge from mild anomalies and enhanced fertility to disrupted gametogenesis, but eventually chronic, enhanced and non-pulsatile action of both FSH and LH leads to female and male infertility and/or hyper- and neoplasias in most of the gonadotropin gain-of-function mice. Elevated gonadotropin levels also alter the function of several extra-gonadal tissues either directly or indirectly via increased sex steroid production. These effects include promotion of tumorigenesis in tissues such as the pituitary, mammary and adrenal glands. Finally, the crossbreedings of the current mouse strains with other disease models are likely to uncover the contribution of gonadotropins in novel biological systems, as exemplified by the recent crossbreed of LHCG receptor deficient mice with Alzheimer disease mice
Correlation of gene expression and protein production rate - a system wide study
<p>Abstract</p> <p>Background</p> <p>Growth rate is a major determinant of intracellular function. However its effects can only be properly dissected with technically demanding chemostat cultivations in which it can be controlled. Recent work on <it>Saccharomyces cerevisiae </it>chemostat cultivations provided the first analysis on genome wide effects of growth rate. In this work we study the filamentous fungus <it>Trichoderma reesei </it>(<it>Hypocrea jecorina</it>) that is an industrial protein production host known for its exceptional protein secretion capability. Interestingly, it exhibits a low growth rate protein production phenotype.</p> <p>Results</p> <p>We have used transcriptomics and proteomics to study the effect of growth rate and cell density on protein production in chemostat cultivations of <it>T. reesei</it>. Use of chemostat allowed control of growth rate and exact estimation of the extracellular specific protein production rate (SPPR). We find that major biosynthetic activities are all negatively correlated with SPPR. We also find that expression of many genes of secreted proteins and secondary metabolism, as well as various lineage specific, mostly unknown genes are positively correlated with SPPR. Finally, we enumerate possible regulators and regulatory mechanisms, arising from the data, for this response.</p> <p>Conclusions</p> <p>Based on these results it appears that in low growth rate protein production energy is very efficiently used primarly for protein production. Also, we propose that flux through early glycolysis or the TCA cycle is a more fundamental determining factor than growth rate for low growth rate protein production and we propose a novel eukaryotic response to this i.e. the lineage specific response (LSR).</p
Two-parameter boundary condition applied to transverse acoustic impedance of a Fermi liquid
Abstract
We discuss surface scattering in terms of bidirectional reflectance distribution function. We formulate a boundary condition that in addition to diffuse and specular reflection has tilted diffuse reflection. We apply the boundary condition to calculate the transverse acoustic impedance of a normal-state Fermi liquid. Some additional results on the impedance in a slab geometry are presented
Models for supercritical motion in a superfluid Fermi liquid
Abstract
We study the drag force on objects moving in a Fermi superfluid at velocities on the order of the Landau velocity vL. The expectation has been that vL is the critical velocity beyond which the drag force starts to increase toward its normal-state value. This expectation is challenged by a recent experiment measuring the heat generated by a uniformly moving wire immersed in superfluid ³He. We introduce the basis for the calculation of the drag force on a macroscopic object using the Fermi-liquid theory of superfluidity. As a technical tool in the calculations, we propose a boundary condition that describes diffuse reflection of quasiparticles from a surface on a scale that is larger than the superfluid coherence length. We calculate the drag force on steadily moving objects of different sizes. For an object that is small compared to the coherence length, we find a drag force that is in accordance with the expectation. For a macroscopic object, we need to take into account the spatially varying flow field around the object. At low velocities, this arises from ideal flow of the superfluid. At higher velocities, the flow field is modified by excitations that are created when the flow velocity locally exceeds vL. The flow field causes Andreev reflection of quasiparticles and thus leads to change in the drag force. We calculate multiple limiting cases for a cylinder-shaped object. In the absence of quasiparticle-quasiparticle collisions, we find that the critical velocity is larger than vL and the drag force (per cross-sectional area) at 2vL is reduced by an order of magnitude compared to the case of a small object. In a collision-dominated limit, the flow shows signs of instability at a velocity belowvL