Evidence of local and regional freshening of Northeast Greenland coastal waters

The supply of freshwater to fjord systems in Greenland is increasing as a result of climate change-induced acceleration in ice sheet melt. However, insight into the marine implications of the melt water is impaired by lack of observations demonstrating the fate of freshwater along the Greenland coast and providing evaluation basis for ocean models. Here we present 13 years of summer measurements along a 120 km transect in Young Sound, Northeast Greenland and show that sub-surface coastal waters are decreasing in salinity with an average rate of 0.12 ± 0.05 per year. This is the first observational evidence of a significant freshening on decadal scale of the waters surrounding the ice sheet and comes from a region where ice sheet melt has been less significant. It implies that ice sheet dynamics in Northeast Greenland could be of key importance as freshwater is retained in southward flowing coastal currents thus reducing density of water masses influencing major deep water formation areas in the Subarctic Atlantic Ocean. Ultimately, the observed freshening could have implications for the Atlantic meridional overturning circulation

Play behaviour positively relates to weight gain, feeding behaviour and drinking behaviour in weaner pigs (Sus scrofa)

Engagement in play behaviour has been associated with the presence of positive affective states and, thus, proposed to be an indicator of positive animal welfare. However, the interpretation of play in animals remains challenging due to the complexity of motivating factors. Accordingly, we aimed to clarify whether Yorkshire × Landrace weaner pigs would engage more in play behaviour the more well-nourished they were by examining the effects of weight gain, feeding behaviour, and drinking behaviour on two types of play behaviour [locomotor-rotational play (LOC) and social play (SOC)]. In total, 24 litters [pigs/litter: (mean ± SD) 13 ± 2] raised under conventional husbandry conditions were included in this study. Each pig was manually weighed within 24 h of birth and on days − 7, 0, 1, 2 relative to the weaning day (day 0) at approximately 26 days of age. All behavioural measures were registered via video at individual level. Visits to feeder and drinker were registered from 07:00 h to 21:59 h on days − 1 and 1 using 2-min interval instantaneous sampling. The proportion of visits to each resource was calculated by dividing the number of scans visiting the resource by the total number of daily scans. The latencies to visit the feeder and drinker within the first 24 h post-weaning were continuously recorded. Both LOC and SOC were registered between 14:00 h and 22:00 h on days − 1, 1 and 2. Both before and after weaning, heavier pigs spent more time performing LOC. Before weaning, heavier pigs spent more time performing SOC. Proportion of visits to the feeder positively related to LOC after weaning. On the day before weaning, the proportion of visits to the drinker positively related to LOC. No clear relationships between the latency to feed and drink after weaning and play behaviour were found. Our study supports the hypothesis that motivation to play is higher when animals are in more stable conditions, e.g., well-nourished, and healthier than under suboptimal conditions. However, the fact that the nutritional measures did not similarly affect LOC and SOC suggests that these two types of play behaviour may be differently affected by the weaning context and questions whether they have the same underlying motivation. This study represents a step toward the validation of play as a positive animal welfare indicator

Automated detection and quantification of contact behaviour in pigs using deep learning

Change in the frequency of contact between pigs within a group may be indicative of a change in the physiological or health status of one or more pigs within a group, or indicative of the occurrence of abnormal behaviour, e.g. tail-biting. Here, we developed a novel framework that detects and quantifies the frequency of interaction, i.e., a pig head to another pig rear, between pigs in groups. The method does not require individual pig tracking/identification and uses only inexpensive camera-based data capturing infrastructure. We modified the architecture of well-established deep learning models and further developed a lightweight processing stage that scans over pigs to score said interactions. This included the addition of a detection subnetwork to a selected layer of the base residual network. We first validated the automated system to score the interactions between individual pigs within a group, and determined an average accuracy of 92.65% ± 3.74%, under a variety of settings, e.g., management set-ups and data capturing. We then applied the method to a significant welfare challenge in pigs, that of the detection of tail-biting outbreaks in pigs and quantified the changes that happen in contact behaviour during such an outbreak. Our study shows that the system is able to accurately monitor pig interactions under challenging farming conditions, without the need for additional sensors or a pig tracking stage. The method has a number of potential applications to the field of precision livestock farming of pigs that may transform the industry

Feasibility of a standardized ultrasound examination in patients with rheumatoid arthritis: A quality improvement among rheumatologists cohort.

BACKGROUND: Quality improvement is important to facilitate valid patient outcomes. Standardized examination procedures may improve the validity of US. The aim of this study was to investigate the learning progress for rheumatologists during training of US examination of the hand in patients with rheumatoid arthritis (RA). METHODS: Rheumatologists with varying degrees of experience in US were instructed by skilled tutors. The program consisted of two days with hands-on training followed by personal US examinations performed in their individual clinics. Examinations were sent to the tutors for quality control. The US examinations were evaluated according to a scoring sheet containing 144 items. An acceptable examination was defined as > 80% correct scores. RESULTS: Thirteen rheumatologists participated in the study. They included a total of 104 patients with RA. Only few of the initial examinations were scored below 80%, and as experience increased, the scores improved (p = 0.0004). A few participants displayed decreasing scores. The mean time spent performing the standardized examination procedure decreased from 34 min to less than 10 minutes (p = 0.0001). CONCLUSION: With systematic hands-on training, a rheumatologist can achieve a high level of proficiency in the conduction of US examinations of the joints of the hand in patients with RA. With experience, examination time decreases, while the level of correctness is maintained. The results indicate that US may be applied as a valid measurement tool suitable for clinical practice and in both single- and multi-centre trials

Carbon bioavailability in a high Arctic fjord influenced by glacial meltwater, NE Greenland

The land-to-ocean flux of organic carbon is increasing in glacierized regions in response to increasing temperatures in the Arctic (Hood et al., 2015). In order to understand the response of the coastal ecosystem metabolism to the organic carbon input it is essential to determine the bioavailability of the different carbon sources in the system.We quantified the bacterial turnover of organic carbon in a high Arctic fjord system (Young Sound, NE Greenland) during the ice-free period (July-October 2014) and assessed the quality and quantity of the 3 major organic carbon sources; (1) local phytoplankton production (2) runoff from land-terminating glaciers and a lowland river and (3) inflow from the ocean shelf. We found that despite relatively low concentrations of DOC in the rivers, the bioavailability of the river–DOC was significantly higher than in the fjord, and characterized by high cell-specific bacterial production and low C:N ratios. In contrast, the DOC source entering via inflow of coastal shelf waters had high DOC concentrations with high C:N and low specific bacterial production. The phytoplankton production in the fjord could not sustain the bacterial carbon demand, but was still the major source of organic carbon for bacterial growth. We assessed the bacterial community composition and found that communities were specific for the different water types i.e., the bacterial community of the coastal inflow water could be traced mainly in the subsurface water, while the glacial river community strongly dominated the surface water in the fjord

Investigating the effects of two weaning methods and two genetic hybrids on play behavior in weaner pigs (Sus scrofa)

In conventional pig production, suckling pigs are typically weaned between 3 and 5 weeks of age. This involves separation from the sow, dietary change, and regrouping in a novel environment, which challenge the welfare of pigs. We investigated the effects of two weaning methods [conventional weaning: two litters mixed in a weaner pen of different size and design (MOVE) vs. litter staying in the farrowing pen after removing the sow (STAY)] and two genetic hybrids [DanBred Yorkshire × Landrace (approximately 21 total pigs born/litter; DB) vs. Topigs Norsvin TN70 Yorkshire × Landrace (approximately 16 total pigs born/litter with higher individual birth weight and weaning weight than DB; TN)] on play behavior across weaning. Both genetic hybrids were inseminated with semen of DanBred Duroc boars. Litters were reduced to the number of functional teats at birth. The durations of locomotor-rotational play (LOC) and social play (SOC) of 24 indoor-housed litters [pigs/litter: (average ± SD) 13 ± 2; age at the weaning day: 26 ± 2 days] were video-recorded continuously between 14:00 h and 22:00 h on days −1, 1, and 2 relative to weaning and statistically analyzed with mixed-effects modeling at the individual level. Before weaning, TN pigs performed LOC longer than DB pigs. On day 2 post-weaning, STAY pigs engaged in more SOC than MOVE pigs. Moreover, TN pigs and STAY pigs displayed a steeper increase in LOC from days 1 to 2 than DB pigs and MOVE pigs, respectively. We demonstrated that pigs belonging to the genetic hybrid with higher weight at birth and weaning spent more time playing on the day before weaning. Additionally, weaning pigs in the farrowing pen and, hence, avoiding social mixing and relocation to an unfamiliar environment had a positive effect on social play after weaning. Our study illustrates that weaning stress in pigs may be reduced by using a genetic hybrid featuring higher birth and weaning weight and by keeping litters intact in a familiar environment after weaning. This study also supports the use of play behavior as an animal welfare indicator

CAF01 Potentiates Immune Responses and Efficacy of an Inactivated Influenza Vaccine in Ferrets

Trivalent inactivated vaccines (TIV) against influenza are given to 350 million people every year. Most of these are non-adjuvanted vaccines whose immunogenicity and protective efficacy are considered suboptimal. Commercially available non-adjuvanted TIV are known to elicit mainly a humoral immune response, whereas the induction of cell-mediated immune responses is negligible. Recently, a cationic liposomal adjuvant (dimethyldioctadecylammonium/trehalose 6,6′-dibehenate, CAF01) was developed. CAF01 has proven to enhance both humoral and cell-mediated immune responses to a number of different experimental vaccine candidates. In this study, we compared the immune responses in ferrets to a commercially available TIV with the responses to the same vaccine mixed with the CAF01 adjuvant. Two recently circulating H1N1 viruses were used as challenge to test the vaccine efficacy. CAF01 improved the immunogenicity of the vaccine, with increased influenza-specific IgA and IgG levels. Additionally, CAF01 promoted cellular-mediated immunity as indicated by interferon-gamma expressing lymphocytes, measured by flow cytometry. CAF01 also enhanced the protection conferred by the vaccine by reducing the viral load measured in nasal washes by RT-PCR. Finally, CAF01 allowed for dose-reduction and led to higher levels of protection compared to TIV adjuvanted with a squalene emulsion. The data obtained in this human-relevant challenge model supports the potential of CAF01 in future influenza vaccines